Abstract
It has been shown that the fungus Lentinus edodes grown on a solid wort agar substrate produces intracellular enzymes, including Mn-dependent peroxidase, laccase, and tyrosinase as a family of isoforms. The composition of the complex (containing one to four forms of each enzyme) varied during the basidiomycete life cycle. The activity of oxidases was maximal at the stage of nonpigmented mycelium and at the stages of a brown mycelial mat and a fruit body. The activity of tyrosinase increased in the course of mycelium pigmentation and had two maxima: at the stage of a brown mycelial mat and at the stage of a fruit body. Laccase and tyrosinase activities were shown to increase sharply upon addition of oak sawdust extract to the culture medium as compared with the enzyme activities of mycelium grown on wort agar alone. It was established that the effect of phenol oxidase substrates on the growing mycelium consists in a twofold acceleration of the process of morphogenesis in the fungus L. edodes.
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References
Bennett, J.W., Secondary metabolism and differentiation in fungi, Bennett, J.W. and Ciegler, A., Eds., New York:-Basel: Marsel Decker Inc., 1983, pp. 1–32.
Sokolovskii, V.Yu. and Belozerskaya, T.A., Effect of Stressors on the Differential Gene Expression in the Course of Neurospora crassa Development, Usp. Biol. Khim., 2000, vol. 40, pp. 85–152.
Reshetnikova, I.A., Destruktsiya lignina ksilotrofnymi makromitsetami. Nakoplenie selena i fraktsionirovanie ego izotopov mikroorganizmami (Lignin Destruction by Xylotrophic Macromycetes. Microbial Accumulation of Selenium and Fractionation of Its Isotopes), Moscow: Novintekh-Press, 1997.
Thurston, C., The Structure and Function of Fungal Laccases, Microbiology (UK), 1994, vol. 140, pp. 19–26.
Baldrian, P., Fungal Laccases-Occurrence and Properties, FEMS Microbiol. Rev., 2006, vol. 20, pp. 1–28.
Van Gelder, C.W.G., Flurkey, W.H., and Wichers, H.J., Sequence and Structural Features of Plant and Fungal Tyrosinases, Phytochemistry, 1997, vol. 45, pp. 1309–1323.
Evans, C., Laccase Activity in Lignin Degradation by Coriolus versicolor. In vivo and in vitro Studies, FEMS Microbiol. Letts., 1985, vol. 27, pp. 339–343.
Maltseva, O.V., Niku-Paavola, M.-L., Leontievsky, A.A., Myasoedova, N.M., and Golovleva, L.A., Ligninolytic Enzymes of the White Rot Fungus Panus tigrinus, Biot. Appl. Biochem., 1991, vol. 13, pp. 291–302.
Vu, T., Blagopriyatnye lechebnye effekty ekzoticheskikh s"edobnykh gribov, in (Favorable Medical Effects of Exotic Edible Mushrooms), Moscow: Byull. MAG, 1996, pp. 12–17.
Konno, K., Biologically Active Components of Poisonous Mushrooms, Food Rev. Intern., 1995, vol. 11, pp. 83–107.
Nizovskaya, O.P., Antitumor Properties of Higher Basidiomycetes, Mikol. Fitopatol., 1983, vol. 17, no. 3, pp. 243–247.
Garibova, L.V., Zav’yalova, L.A., Aleksandrova, E.A., and Nikitina, V.E., Biology of Lentinus edodes. I. Morphologo-Cultural and Physiologo-Biochemical Characteristics, Mikol. Fitopatol., 1999, vol. 33, no. 2, pp. 107–110.
Bilai, V.I., Metody eksperimental’noi mikologii (Methods in Experimental Mycology), Kiev: Naukova dumka, 1982.
Przybylowicz, P. and Donoghue, J., Shiitake Growers Handbook: the Art and Science of Mushroom Cultivation, Dubuque: Kendall, 1991.
Stamets, P., Growing Gourmet and Medicinal Mushrooms, Berkeley: Ten Speed Press, 1993.
Niku-Paavola, M.-L., Karhunen, E., Salola, P., and Paunio, V., Ligninolytic Enzymes of the White Rot Fungus Phlebia radiata, Biochem. J., 1988, vol. 254, pp. 877–302.
Slomczynski, D., Nakas, J.P., and Tanenbaum, S.W., Production and Characterization of Laccase from Botrytis cinerea 61–34, Appl. Environ. Microbiol., 1995, vol. 61, pp. 907–912.
Leonowicz, A. and Crzywnowicz, K., Quantitative Estimation of Laccase Forms in Some White-Rot Fungi Using Syringaldazine As a Substrate, Enz. Microbiol. Technol., 1981, vol. 3, pp. 55–58.
Glenn, J.K. and Gold, M.H., Purification and Characterization of an Extracellular Mn(II)-Dependent Peroxidase from the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium, Arch. Biochem. Biophys., 1985, vol. 242, pp. 329–341.
Pomerantz, S.M. and Murthy, V.V., Purification and Properties of Tyrosinases from Vibrio tyrosinaticus, Arch. Biochem. Biophys., 1974, vol. 160, pp. 73–82.
Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Qualities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.
Laemmli, U.K., Cleavage of Structural Proteins during the Assembly of the Head Bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680–685.
Gaal’, E., Med’eshi, G., and Veretski, L., Elektroforez v razdelenii biologicheskikh makromolekul (Electrophoresis in the Separation of Biological Macromolecules), Moscow: Mir, 1982.
Gauslaa, Y. and Solhaug, K.A., Fungal Melanins as a Sun Screen for Symbiotic Green Algae in the Lichen Lobaria pulmonaria, Oecologia, 2001, vol. 126, pp. 462–471.
Rikkinen, J., Wat’s Behind the Pretty Colours? A Study on the Photobiology of Lichens, Bryobrothera, 1995, vol. 4, pp. 1–239.
Jonsson, L., Karlsson, O., Lundquist, K., and Nyman, P.O., Trametes versicolor Ligninase: Isozyme Sequence Homology and Substrate Specificity, FEBS Lett., 1989, vol. 247, pp. 143–146.
Urzua, U., Larrondo, L.F., Lobos, S., Larrain, J., and Vicuna, R., Oxidation Reactions Catalyzed by Manganese Peroxidase Isoenzyme from Ceriporiopsis subvermispora, FEBS Lett., 1995, vol. 371, pp. 132–136.
Fukushima, Y. and Kirk, T.K., Laccase Component of the Ceriporiopsis subvermispora Lignin-Degrading System, App. Environ. Microbiol., 1995, vol. 61, pp. 872–876.
Nunolstein, C., Valenti, P., Visca, P., Antonini, G., Nicolini, L., and Orsi, N., Production of Laccases A and B by Mutant Strain of Trametes versicolor, J. Gen. Microbiol., 1986, vol. 32, pp. 185–191.
Leatham, G.F. and Stahmann, M.A., Studies on the Laccase of Lentinus edodes: Specificity, Localization and Association with the Development of Fruiting Bodies, J. Gen Microbiol., 1981, vol. 125, pp. 147–157.
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Original Russian Text © E.P. Vetchinkina, N.N. Pozdnyakova, V.E. Nikitina, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 2, pp. 171–177.
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Vetchinkina, E.P., Pozdnyakova, N.N. & Nikitina, V.E. Enzymes of the xylotrophic basidiomycete Lentinus edodes F-249 in the course of morphogenesis. Microbiology 77, 144–150 (2008). https://doi.org/10.1134/S0026261708020045
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DOI: https://doi.org/10.1134/S0026261708020045