Skip to main content
SpringerLink
Log in

Fractions of barley spent grain as media for growth of probiotic bacteria

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

The application of the protein and polysaccharide fractions of barley spent grain as a basis of growth media for probiotic bacteria was studied. High values of biomass yield, cell viability, and organic acid production were observed in the variants of media containing the barley spent grain supplemented with lactose, ascorbic acid, yeast extract, and mineral salts. Cells of lactic acid bacteria and bifidobacteria had the typical rod-shaped morphology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Reid, G., Jass, J., Sebulsky, M.T., and McCormick, J.K., Potential Uses of Probiotics in Clinical Practice, Clin. Microbiol. Rev., 2003, vol. 16, pp. 658–672.

    Article  PubMed  Google Scholar 

  2. Novik, G.I., Astapovich, N.I., Bogdanovskaya, Zh.N., and Ryabaya, N.E., Production of the Medicinal and Prophylactic Preparation Enterobifidin Using Bifidobacterium adolescentis MS-42, Prikl. Biokhim. Mikrobiol., 2000, vol. 36, no. 1, pp. 104–110 [App. Biochem. Microbiol. (Engl. Transl.), vol. 36, no. 1, pp. 90–95].

    PubMed  CAS  Google Scholar 

  3. Novik, G.I., Structural and Functional Organization of Bifidobacteria, Mikrobiologiya, 1998, vol. 67, no. 3, pp. 376–383 [Microbiology (Engl. Transl.), vol. 67, no. 3, pp. 310–316].

    CAS  Google Scholar 

  4. Ventura, M., Sinderen, D., Fitzgerald, G.F., and Zink, R., Insights into the Taxonomy, Genetics and Physiology of Bifidobacteria, Antonie van Leeuwenhoek, 2004, vol. 86, pp. 205–223.

    Article  PubMed  CAS  Google Scholar 

  5. Charalampopoulos, D., Pandiella, S.S., and Webb, C., Growth Studies of Potentially Probiotic Lactic Acid Bacteria in Cereal-Based Substrates, J. Appl. Microbiol., 2002, vol. 92, pp. 851–859.

    Article  PubMed  CAS  Google Scholar 

  6. Bamba, T., Kanauchi, O., Andoh, A., and Fujiyama, Y., A New Prebiotic from Germinated Barley for Nutraceutical Treatment of Ulcerative Colitis, J. Gastroenterol. Hepatol., 2002, vol. 17, pp. 818–824.

    Article  PubMed  CAS  Google Scholar 

  7. Kanauchi, O., Serizawa, I., Araki, Y., Suzuki, A., Andon, A., Fujiyama, Y., Mitsuyama, K., Takaki, K., Toyonaga, A., Sata, M., and Bamba, T., Germinated Barley Foodstuff, a Prebiotic Product, Ameliorates Inflammation of Colitis through Modulation of the Enteric Environment, J. Gastroenterol., 2003, vol. 38, pp. 134–141.

    Article  PubMed  CAS  Google Scholar 

  8. Charalampopoulos, D., Wang, R., Pandiella, S.S., and Webb, C., Application of Cereals and Cereal Components in Functional Foods: a Review, Int. J. Food Microbiol., 2002, vol. 15, pp. 131–141.

    Article  Google Scholar 

  9. Lasztity, R., Barley Proteins, The Chemistry of Cereal Proteins, CRC Press, 1984, pp. 117–126.

  10. Ishiwaki, N., Muruyama, H., Awayama, H., Kanauchi, O., and Sato, T., Development of High Value Uses of Spent Grain by Fractionation Technology, Tech. Quart, 2000, vol. 37, pp. 261–265.

    CAS  Google Scholar 

  11. Dongowski, G., Huth, M., Gebhardt, E., and Flamme, W., Dietary Fiber-Rich Barley Products Beneficially Affect the Intestinal Tract of Rats, J. Nutr., 2002, vol. 132, pp. 3704–3714.

    PubMed  CAS  Google Scholar 

  12. Schrezenmeir, J. and de Vrese, M., Probiotics, Prebiotics, and Synbiotics — Approaching a Definition, Am. J. Clin. Nutr., 2001, vol. 73, pp. 361–364.

    Google Scholar 

  13. Szponar, B., Pawlik, K.J., Gamian, A., and Szwajcer Dey, E., Protein Fraction of Barley Spent Grain as a New Simple Medium for Growth and Sporulation of Soil Actinobacteria, Biotechnol. Lett., 2003, vol. 25, pp. 1717–1721.

    Article  PubMed  CAS  Google Scholar 

  14. Novik, G.I., Astapovich, N.I., and Samartsev, A.A., Investigation of the Physiological and Biochemical Characteristics of Bifidobacteria at the Late Stages of Their Development, Mikrobiologiya, 2001, vol. 70, no. 4, pp. 495–502 [Microbiology (Engl. Transl.), vol. 70, no. 4, pp. 429–435].

    CAS  Google Scholar 

  15. Lauer, E. and Kandler, O., Mechanismus der Variation des Verhalthisses Acetat/Lactat bei der Vergarung von Glucose durch Bifidobacterien, Arch. Microbiol., 1976, vol. 110, pp. 271–277.

    Article  PubMed  CAS  Google Scholar 

  16. Novik, G.I., Astapovich, N.I., Kubler, I., and Gamian, A., Characterization of the Cell-Bound Polysaccharides of Bifidobacterium adolescentis 94 BIM, Mikrobiologiya, 2002, vol. 71, no. 2, pp. 205–210 [Microbiology (Engl. Transl.), vol. 71, no. 2, pp. 173–177].

    CAS  Google Scholar 

  17. Szwajcer, DeyE., Rasmussen, J., and Breddam, K., Proline Specific Endopeptidases from Microbial Sources: Isolation of an Enzyme from Xanthomonas sp., J. Bacteriol., 1992, vol. 174, pp. 2454–2459.

    Google Scholar 

  18. Novik, G., Gamian, A., Cruz, Francisco J., and Szwajcer Dey, E., A Novel Procedure for the Isolation of Glycolipids from Bifidobacterium adolescentis 94 BIM Using Supercritical Carbon Dioxide, J. Biotechnol., 2006, vol. 121, pp. 555–562.

    Article  PubMed  CAS  Google Scholar 

  19. Kleyn, J. and Hough, J., The Microbiology of Brewing, Annu. Rev. Microbiol., 1971, vol. 25, pp. 583–608.

    Article  PubMed  CAS  Google Scholar 

  20. Das, H. and Singh, S.K., Useful Byproducts from Cellulosic Wastes of Agriculture and Food Industry—a Critical Appraisal, Crit. Rev. Food Scie Nut., 2004, vol. 44, pp. 77–89.

    Article  Google Scholar 

  21. Hosono, A., Lee, J., Ametani, A., Natsume, M., Hirayama, M., Adachi, T., and Kaminogawa, S., Characterization of a Water-Soluble Polysaccharide Fraction with Immunopotentiating Activity from Bifidobacterium adolescentis M101-4, Biosci. Biotechnol. Biochem., 1997, vol. 61, pp. 312–316.

    Article  PubMed  CAS  Google Scholar 

  22. Crittenden, R., Laitila, A., Forssell, P., Mättö, J., Saarela, M., Mattila-Sandholm, T., and Myllarinen, P., Adhesion of Bifidobacteria to Granular Starch and Its Implications in Probiotic Technologies, Appl. Environ. Microbiol., 2001, vol. 67, pp. 3469–3475.

    Article  PubMed  CAS  Google Scholar 

  23. Shukla, T.P., Nutraceutical Novelties: Trends in Proprietary Technology, Cereal Foods World, 1998, vol. 43, pp. 388–389.

    Google Scholar 

  24. Kanauchi, O., Fujiyama, Y., Mitsuyama, K., Araki, Y., Ishii, T., Nakamura, T., Hitomi, Y., Agata, K., Saiki, T., Andoh, A., Toyonaga, A., and Bamba, T., Increased Growth of Bifidobacterium and Eubacterium by Germinated Barley Foodstuff, Accompanied by Enhanced Butyrate Production in Health Volunteers, Jnt. J. Mol. Med., 1999, vol. 3, pp. 175–179.

    CAS  Google Scholar 

  25. Janer, C., Arigoni, F., Lee, B.H., Pelaez, C., and Requena, T., Enzymatic Ability of Bifidobacterium animalis subsp. lactis to Hydrolyze Milk Proteins: Identification and Characterization of Endopeptidase O, Appl. Environ. Microbiol., 2005, vol. 71, pp. 8460–8465.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, National Academy of Sciences of Belarus, ul. Kuprevicha 2, Minsk, 220141, Belarus

    G. I. Novik

  2. Department of Pure and Applied Biochemistry, Lund University, Lund, Sweden

    J. Wawrzynczyk, O. Norrlow & E. Szwajcer-Dey

Authors
  1. G. I. Novik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Wawrzynczyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Norrlow
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Szwajcer-Dey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. I. Novik.

Additional information

Original Russian Text © G.I. Novik, J. Wawrzynczyk, O. Norrlow, E. Szwajcer-Dey, 2007, published in Mikrobiologiya, 2007, Vol. 76, No. 6, pp. 902–907.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Novik, G.I., Wawrzynczyk, J., Norrlow, O. et al. Fractions of barley spent grain as media for growth of probiotic bacteria. Microbiology 76, 804–808 (2007). https://doi.org/10.1134/S0026261707060227

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261707060227

Key words

Search

Navigation