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Halomonas mongoliensis sp. nov. and Halomonas kenyensis sp. nov., new haloalkaliphilic denitrifiers capable of N2O reduction, isolated from soda lakes

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Abstract

In the course of the search for N2O-utilizing microorganisms, two novel strains of haloalkaliphilic denitrifying bacteria, Z-7009 and AIR-2, were isolated from soda lakes of Mongolia and Kenya. These microorganisms are true alkaliphiles and grow in the pH ranges of 8.0–10.5 and 7.5–10.6, respectively. They are facultative anaerobes with an oxidative type of metabolism, able to utilize a wide range of organic substrates and reduce nitrate, nitrous oxide, and, to a lesser extent, nitrite to gaseous nitrogen. They can oxidize sulfide in the presence of acetate as the carbon source and nitrous oxide (strain Z-7009) or nitrate (strain AIR-2) as the electron acceptor. The strains require Na+ ions. They grow at 0.16–2.2 M Na+ (Z-7009) and 0.04–2.2 M Na+ (AIR-2) in the medium. The G+C contents of the DNA of strains Z-7009 and AIR-2 are 67.9 and 65.5 mol %, respectively. According to the results of 16S rRNA gene sequencing and DNA-DNA hybridization, as well as on the basis of physiological properties, the strains were classified as new species of the genus Halomonas: Halomonas mongoliensis, with the type strain Z-7009T (=DSM 17332, =VKM B2353), and Halomonas kenyensis, with the type strain AIR-2T (=DSM 17331, =VKM B2354).

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    Yu. V. Boltyanskaya, V. V. Kevbrin, A. M. Lysenko, T. P. Tourova & T. N. Zhilina

  2. Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 1, Moscow, 117312, Russia

    T. V. Kolganova

  3. Bakulev Scientific Center for Cardiovascular Surgery, Russian Academy of Medical Sciences, Rublevskoe Shosse 135, Moscow, 121552, Russia

    G. A. Osipov

Authors
  1. Yu. V. Boltyanskaya
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  2. V. V. Kevbrin
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  3. A. M. Lysenko
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  4. T. V. Kolganova
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  5. T. P. Tourova
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  6. G. A. Osipov
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  7. T. N. Zhilina
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Correspondence to Yu. V. Boltyanskaya.

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Original Russian Text © Yu.V. Boltyanskaya, V.V. Kevbrin, A.M. Lysenko, T.V. Kolganova, T.P. Tourova, G.A. Osipov, T.N. Zhilina, 2007, published in Mikrobiologiya, 2007, Vol. 76, No. 6, pp. 834–843.

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Boltyanskaya, Y.V., Kevbrin, V.V., Lysenko, A.M. et al. Halomonas mongoliensis sp. nov. and Halomonas kenyensis sp. nov., new haloalkaliphilic denitrifiers capable of N2O reduction, isolated from soda lakes. Microbiology 76, 739–747 (2007). https://doi.org/10.1134/S0026261707060148

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