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Formation of the exopolysaccharide ethapolan by Acinetobacter sp. IMV B-7005 on a fumarate-glucose mixture

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Abstract

Our studies enabled us to intensify the synthesis of the microbial exopolysaccharide (EPS) ethapolan produced by Acinetobacter sp. IMV B-7005 grown on a mixture of fumarate (an energy-excessive substrate) and glucose (an energy-deficient substrate). Supplementing glucose-containing medium with sodium (potassium) fumarate at a molar ratio of 4: 1 resulted in a 1.3–2.2-fold increase of the EPS amount synthesized and in a 1.3–2-fold increase of the EPS yield relative to the biomass compared to cultivation on monosubstrates. The conversion of the carbon of both substrates to EPS was the highest if the carbon/nitrogen ratio in the cultivation medium was 70.5 and inoculum grown on glucose monosubstrate was used.

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References

  1. Pirog, T.P., Kovalenko, M.A., Kuz’minskaya, Yu.V., and Krishtab, T.P., Enhanced Synthesis of the Exopolysaccharide Ethapolan by Acinetobacter sp. 12S Grown on a Mixture of Substrates, Mikrobiologiya, 2003, vol. 72, no. 1, pp. 26–32 [Microbiology (Engl. Transl.), vol. 72, no. 1, pp. 305–312].

    CAS  Google Scholar 

  2. Pirog, T.P., Kovalenko, M.A, and Kuz’minskaya, Yu.V., Intensification of Exopolysaccharide Synthesis by Acinetobacter sp. on an Ethanol-Glucose Mixture: Aspects Related to Biochemistry and Bioenergetics, Mikrobiologiya, 2003, vol. 72, no. 3, pp. 348–355 [Microbiology (Engl. Transl.), vol. 72, no. 3, pp. 305–312].

    CAS  Google Scholar 

  3. Pirog, T.P., Kuz’minskaya, Yu.V., and Kovalenko, M.O., Metabolism of C2-C6 Substrates by Mixotrophically Grown Acinetobacter sp. Strains B-7005 and B-7005 (1NG), Ukr. Biokhim. Zh., 2004, vol. 76, no. 1, pp. 33–38.

    CAS  Google Scholar 

  4. Pirog, T.P., Kovalenko, M.A., Kuz’minskaya, Yu.V., and Votselko, S.K., Physicochemical Properties of the Microbial Exopolysaccharide Ethapolan Synthesized on a Mixture of Growth Substrates, Mikrobiologiya, 2004, vol. 73, no. 1, pp. 19–24 [Microbiology (Engl. Transl.), vol. 73, no. 1, pp. 14–18].

    CAS  Google Scholar 

  5. Pirog, T.P. and Kuz’minskaya, Yu.V., Some Characteristics of Central Metabolism in Acinetobacter sp. Grown on Ethanol, Mikrobiologiya, 2003, vol. 72, no. 4, pp. 459–465 [Microbiology (Engl. Transl.), vol. 72, no. 4, pp. 408–413].

    CAS  Google Scholar 

  6. Babel, W. and Muller, R.H., Mixed Substrate Utilization in Microorganisms: Biochemical Aspects and Energetics, J. Gen. Microbiol., 1985, vol. 131, no. 1, pp. 39–45.

    CAS  Google Scholar 

  7. Grinberg, T.A., Pirog, T.P., Malashenko, Yu.R., and Pinchuk, G.E., Mikrobnyi sintez ekzopolisakharidov na C 1 -C 2 -soedineniyakh (Microbial Synthesis of Exopolysaccharides on C1-C2 Compounds), Kiev: Nauk. dumka, 1992.

    Google Scholar 

  8. Lukomskaya, I.S. and Gorodetskii, V.K., Application of Microcide (Glucose Oxidase) for Determination of Glucose Levels in Normal and Diabetic Blood, Biokhimiya, 1961, vol. 26, no. 3, pp. 477–482.

    Google Scholar 

  9. Pyatnitskii, M.P. and Yur’eva, A.F., Fumarate Method for Determination of Malic Acid, Biokhimiya, 1949, vol. 14, no. 3, pp. 196–200.

    CAS  Google Scholar 

  10. Jarman, T.R. and Pace, G.W., Energy Requirements for Microbial Exopolysaccharide Synthesis, Arch. Microbiol., 1984, vol. 137, pp. 231–235.

    Article  CAS  Google Scholar 

  11. Pirog, T.P., Vysyatetskaya, N.V., and Korzh, Yu.V., Specific Features of the Synthesis of the Exopolysaccharide Ethapolan on a Mixture of Energy-Deficient Growth Substrates, Mikrobiologiya, 2007, vol. 76, no. 1, pp. 32–38 [Microbiology (Engl. Transl.), vol. 76, no. 1, pp. 25–30].

    CAS  Google Scholar 

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Correspondence to T. P. Pirog.

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Original Russian Text © T.G. Pirog, N.V. Vysyatetskaya, Yu.V. Korzh, 2007, published in Mikrobiologiya, 2007, Vol. 76, No. 6, pp. 790–796.

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Pirog, T.P., Vysyatetskaya, N.V. & Korzh, Y.V. Formation of the exopolysaccharide ethapolan by Acinetobacter sp. IMV B-7005 on a fumarate-glucose mixture. Microbiology 76, 698–703 (2007). https://doi.org/10.1134/S0026261707060070

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  • DOI: https://doi.org/10.1134/S0026261707060070

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