Skip to main content
SpringerLink
Log in

Composition, structure, and biological properties of lipopolysaccharides from different strains of Pseudomonas syringae pv. atrofaciens

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

The composition, structure, and certain biological properties of lipopolysaccharides (LPS) isolated from six strains of bacteria Pseudomonas syringae pv. atrofaciens pathogenic for grain-crops (wheat, rye) are presented. The LPS-protein complexes were isolated by a sparing procedure (extraction from microbial cells with a weak salt solution). They reacted with the homologous O sera and contained one to three antigenic determinants. Against the cells of warm-blooded animals (mice, humans) they exhibited the biological activity typical of endotoxins (stimulation of cytokine production, mitogenetic activity, etc.). The LCD of the biovar type strain was highly toxic to mice sensitized with D-galactosamine. The structural components of LPS macromolecules obtained by mild acidic degradation were characterized: lipid A, core oligosaccharide, and O-specific polysaccharide (OPS). Fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1 were identified in lipid A of all the strains, as well as the components of the hydrophilic part: glucosamine (GlcN), ethanolamine (EtN), phosphate, and phosphoethanolamine (EtN-P). In the core LPS, glucose (Glc), rhamnose (Rha), L-glycero-D-manno-heptose (Hep), GlcN, galactosamine (GalN), 2-keto-3-deoxy-D-mannooctonoic acid (KDO), alanine (Ala), and phosphate were present. The O chain of all the strains consisted of repeated elements containing a linear chain of three to four L-(two strains) or D-Rha (four strains) residues supplemented with a single residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3Nac), N-acetyl-D-glucosamine (D-GlcpNAc), D-fucose (D-Fucf), or D-Rhap (strain-dependent) as a side substituent. In different strains the substitution position for Rha residues in the repeated components of the major rhamnan chain was also different. One strain exhibited a unique type of O-chain heterogeneity. Immunochemical investigation of the LPS antigenic properties revealed the absence of close serological relations between the strains of one pathovar; this finding correlates with the differences in their OPS structure. Resemblance between the investigated strains and other P. syringae strains with similar LPS structures was revealed. The results of LPS analysis indicate the absence of correlation between the OPS structure and the pathovar affiliation of the strains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Palleroni, N.J., Pseudomonadaceae, Bergey’s Manual of Systematic Bacteriology, Holt, J.G., Ed., Baltimore: Williams and Wilkins, 1984, vol. 1, pp. 141–219.

    Google Scholar 

  2. Koroleva, I.B. and Pasichnik, L.A., Susceptibility of Different Varieties of Rye and Other Grain Crops to Pseudomonas syringae pv. atrofaciens, Mikrobiol. Zh., 1989, vol. 51, no. 1, pp. 98–100.

    Google Scholar 

  3. Gardan, L., Shafik, H., Belouin, S., Broch, R., Grimont, F., and Grimont, P.A.D., DNA Relatedness Among the Pathovars of Pseudomonas syringae and Description of Pseudomonas tremae sp. nov. and Pseudomonas connabina sp. nov. (Ex Sutic Dowson 1959), Int. Syst. Bacteriol., 1999, vol. 49, pp. 469–478.

    Article  CAS  Google Scholar 

  4. Otta, J.D. and English, H., Serology and Pathology of Pseudomonas syringae, Phytopathology, vol. 61, no. 5, pp. 443–453.

  5. Pastushenko, L.T. and Simonovich, L.D., Serological Groups of the Pseudomonas Phytopathogenic Bacteria, Mikrobiol. Zh., 1979, vol. 41, no. 4, pp. 330–339.

    PubMed  CAS  Google Scholar 

  6. Ovod, V., Ashorn, P., Yakovleva, L., and Krohn, K., Classification of Pseudomonas syringae with Monoclonal Antibodies Against the Core and O-Side Chains of the Lipopolysaccharide, Phytopathology, 1995, vol. 85, pp. 226–232.

    Article  CAS  Google Scholar 

  7. Saunier, M., Malandrin, L., and Samson, R., Distribution of Pseudomonas syringae Pathovars into Twenty-Three O Serogroups, Appl. Environ. Microbiol., 1996, vol. 62, pp. 2360–2374.

    PubMed  CAS  Google Scholar 

  8. Pasichnik, L.A., Epiphytic Phase of Existence of Pseudomonas syringae pv. coronafaciens, a Pathogen Causing Oats Aureole Bacteriosis, Mikrobiol. Zh., 1999, vol. 41, no. 6, pp. 9–14.

    Google Scholar 

  9. Zdorovenko, G., Gubanova, N., Solyanik, L., Knirel, Y., Yakovleva, L., and Zakharova, I., Composition and Structure of Lipopolysaccharides from the Strains of Different Pathovars of Pseudomonas syringae, Proc. 4th Int. Working Group on Pseudomonas syringae Pathovars, Stamperia Granducale, Florence, 1991, pp. 391–401.

    Google Scholar 

  10. Knirel, Y.A. and Zdorovenko, G.M., Structures of O-Polysaccharide Chains of Lipopolysaccharides As the Basis for Classification of Pseudomonas syringae and Related Strains, Pseudomonas syringae Pathovars and Related Pathogens, Proc. 5th Intern. Working Group Pseudomonas syringae Pathovars and Related Pathogens, Berlin, September 3–8, 1995, Dodrecht: Kluwer, 1997, pp. 475–480.

    Google Scholar 

  11. Zdorovenko, G.M., Shashkov, A.S., Zdorovenko, E.L., Kocharova, N.A., Yakovleva, L.M., Knirel’, Yu.A., and Rudol’f, K., Characterization of the Lipopolysaccharide and Structure of the O-Specific Polysaccharide of the Bacterium Pseudomonas syringae pv. atrofaciens IMV 948, Biokhimiya, 2001, vol. 66, no. 4, pp. 369–377 [Biochemistry (Moscow (Engl. Transl.), vol. 66, no. 4, pp. 369–377].

    CAS  Google Scholar 

  12. Zdorovenko, G.M., Gvozdyak, R.I., Gubanova, N.Ya., Afonina, G.B., and Zdorovenko, E.L., Characterization of the Lipopolysaccharide from a Pseudomonas fluorescens. Biovar I Strain, Mikrobiologiya, 1999, vol. 68, no. 3, pp. 330–339 [Microbiology (Engl. Transl.), vol. 68, no. 3, pp. 281–289].

    CAS  Google Scholar 

  13. Zdorovenko, G.M., Solyanik, L.P., Yakovleva, L.M., and Paramonov, N.A., Characterization of the O Antigenes of Different Strains of Pseudomonas syringae pv. tabaci, Biokhimiya, 1997, vol. 62, pp. 28–37.

    CAS  Google Scholar 

  14. Veremeichenko, S.N., Vodyanik, M.A., and Zdorovenko, G.M., Structural Characteristics and Biological Properties of Pseudomonas fluorescens Lipopolysaccharides, Prikl. Biokhim. Mikrobiol., 2005, vol. 41, no. 4, pp. 414–421 [Appl. Biochem. Microbiol. (Engl. Transl.), vol. 41, no. 4, pp. 365–371].

    Google Scholar 

  15. Zdorovenko, G.M., Veremeichenko, S.N., and Solyanik, L.P., Fatty Acid Composition and Components of the Hydrophilic Part of Lipid A in the Pseudomonas syringae Lipopolysaccharide, Biokhimiya, 1995, vol. 60, pp. 787–793

    Google Scholar 

  16. Zdorovenko, E.L., Zatonsky, G.V., Zdorovenko, G.M., Pasichnik, L.A., Shashkov, A.S., and Knirel, Y.A., Structural Heterogeneity in the Lipopolysaccharides of Pseudomonas syringae with O-Polysaccharide Chains Having Different Repeating Units, Carbohydr. Res., 2001, vol. 336, pp. 329–336.

    Article  PubMed  CAS  Google Scholar 

  17. Yakovleva, L.M., Antigenic Composition of the Pseudomonas phytopathogenic Bacteria of the IV Serogroup, Mikrobiol. Zh., 1986, vol. 48, no. 5, pp. 35–40.

    Google Scholar 

  18. Yakovleva, L.M., Gubanova, N.Ya., Zdorovenko, G.M., Solyanik, L.P., and Gvozdyak, R.I., Immunochemical Research on the Lipopolysaccharides of Pseudomonas syringae pv. atrofaciens, VIII All-Union Conf. “Khimiya i biokhimiya uglevodov” (Carbohydrate Chemistry and Biochemistry), Tbilisi, November 17–19, 1987, Pushchino, 1987, pp. 157–158.

  19. Pasichnik, L.A., Koroleva, I.B., and Gvozdyak, R.I., Serological Properties of Pseudomonas syringae pv. atrofaciens, a Rye Bacteriosis Bathogen, Mikrobiol. Zh., 1991, vol. 53, no. 6, pp. 82–87.

    Google Scholar 

  20. Pasichnik, L.A., Antigenic Properties of the P. syringae Pathovars, Pathogens of Grain Crops, Mikrobiol. Zh., 2000, vol. 62, no. 5, pp. 18–22.

    Google Scholar 

  21. Zdorovenko, G.M. and Veremeichenko, S.N., Comparative Characterization of the Lipopolysaccharides of Different Pseudomonas fluorescens Biovar I Strains, Mikrobiologiya, 2001, vol. 70, no. 4, pp. 509–518 [Microbiology (Engl. Transl., vol. 70, no. 4, pp. 441–450].

    CAS  Google Scholar 

  22. Gross, M., Mayer, H., and Rudolph, K., Comparative Analysis of the Lipopolysaccharides of a Rough and a Smooth Strain of Pseudomonas syringae pv. phaseolicola, Arch. Microbiol., 1988, vol. 149, pp. 372–376.

    Article  CAS  Google Scholar 

  23. Knirel, Y.A., Helbig, J.H., and Zahringer, U., Structure of a Disaccharide Isolated by Mild Acid Degradation and Dephosphorylation of the Lipopolysaccharide of Pseudomonas fluorescens Strain ATCC 4927, Carbohydr. Res., 1996, vol. 283, pp. 129–139.

    Article  PubMed  CAS  Google Scholar 

  24. Knirel’, Yu.A., Zdorovenko, G.M., Dashunin, V.M., Yakovleva, L.M., Shashkov, A.S., Zakharova, I.Ya., Gvozdyak, R.I., and Kochetkov, N.K., Bacterial Antigenic Polysaccharides. 15. Structure of the Repetitive Component of the O-Specific Polysaccharide Chain of Pseudomonas wieringae and Some Pseudomonas syringae Pathovars, Bioorg. Khim., 1986, no. 9, pp. 1253–1262.

  25. Knirel’, Yu.A., Zdorovenko, G.M., Shashkov, A.S., Gubanova, N.Ya., Yakovleva, L.M., and Gvozdyak, R.I., Bacterial Antigenic Polysaccharides. 27. Structure of the LPS O-Specific Polysaccharide Chain of Pseudomonas syringae pv. atrofaciens 2399, pv. phaseolicola 120a, P. holci 8299, Belonging to the VI Serogroup, Bioorg. Khim., 1988, vol. 14, no. 1, pp. 92–99.

    PubMed  CAS  Google Scholar 

  26. Knirel’, Yu.A., Zdorovenko, G.M., Yakovleva, L.M., Shashkov, A.S., Solyanik, L.P., and Zakharova, I.Ya., Bacterial Antigenic Polysaccharides. 28. Structure of the LPS O-Specific Polysaccharide Chain of Pseudomonas syringae pv. atrofaciens K1025 and Pseudomonas holci 90a (Serogroup II), Bioorg. Khim., 1988, vol. 14, no. 2, pp. 166–171.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Russia

    G. M. Zdorovenko & L. D. Varbanets

  2. Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

    E. L. Zdorovenko

Authors
  1. G. M. Zdorovenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. L. Zdorovenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. D. Varbanets
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. M. Zdorovenko.

Additional information

Original Russian Text © G.M. Zdorovenko, E.L. Zdorovenko, L.D. Varbanets, 2007, published in Mikrobiologiya, 2007, Vol. 76, No. 6, pp. 774–789.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zdorovenko, G.M., Zdorovenko, E.L. & Varbanets, L.D. Composition, structure, and biological properties of lipopolysaccharides from different strains of Pseudomonas syringae pv. atrofaciens . Microbiology 76, 683–697 (2007). https://doi.org/10.1134/S0026261707060069

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261707060069

Key words

Search

Navigation