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A new phenotypic manifestation of deletion of the BGL2 gene encoding the cell-wall glucanotransferase Bgl2p in the yeast Saccharomyces cerevisiae

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Abstract

Deletion of the gene encoding the cell-wall glucanotransferase Bgl2p in Saccharomyces cerevisiae decreases the number of dead cells in the yeast culture incubated in a liquid nutrient medium for more than two days. After storage for three months, only 32% of the wild-type cells were found to be able to produce colonies, whereas all cells with the inactivated BGL2 gene retained this ability. It is suggested that the glucanotransferase Bgl2p plays an important role in the limitation of the reproductive life span of aging yeast cells.

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References

  1. Knorre, D.A., Smirnova, E.A., and Severin, F.F., Natural Conditions for the Programmed Death of Saccharomyces cerevisiae Cells, Biokhimiya, 2005, vol. 70, no. 2, pp. 323–326.

    Google Scholar 

  2. Skulachev, V.P., The Programmed Death Phenomena, Aging, and the Samurai Law of Biology, Exp. Gerontol., 2001, vol. 36, pp. 995–1024.

    Article  PubMed  CAS  Google Scholar 

  3. Mrsa, V., Klebl, F., and Tanner, W., Purification and Characterization of the Saccharomyces cerevisiae BGL2 Gene Product, a Cell Wall Endo-beta-1.3-glucanase, J. Bacteriol., 1993, vol. 175, no. 7, pp. 2102–2106.

    PubMed  CAS  Google Scholar 

  4. Klebl, F. and Tanner, W., Molecular Cloning of a Cell Wall Exo-beta-1,3-glucanase from Saccharomyces cerevisiae, J. Bacteriol., 1989, vol. 171, no. 11, pp. 6259–6264.

    PubMed  CAS  Google Scholar 

  5. Kalebina, T.S., Farkas, V., Laurinavichiute, D.K., Gorlovoy, P.M., Fominov, G.V., Bartek, P., and Kulaev, I.S., Deletion of BGL2 Results in an Increased Chitin Level in the Cell Wall of Saccharomyces cerevisiae, Antonie van Leeuwenhoek, 2003, vol. 84, no. 3, pp. 179–184.

    Article  PubMed  CAS  Google Scholar 

  6. Laurinavichyute, D.K., Bovin, N.V., Nasonov, V.V., Morenkov, O.S., Kalebina, T.S., and Kulaev, I.S., The Effect of the BGL2 Gene Mutation on Structural Changes in the Cell Wall of the Yeast Saccharomyces cerevisiae, Dokl. Akad. Nauk, 2000, vol. 372, no. 3, pp. 407–409.

    Google Scholar 

  7. Cappellaro, C., Mrsa, V., and Tanner, W., New Potential Cell Wall Glucanases of Saccharomyces cerevisiae and Their Involvement in Mating, J. Bacteriol., 1998, vol. 180, no. 19, pp. 5030–5037.

    PubMed  CAS  Google Scholar 

  8. Sestak, S., Hagen, I., Tanner, W., and Strahl, S., Scw10p, a Cell Wall Glucanase/Transglucosidase Important for Cell Wall Stability in Saccharomyces cerevisiae, Microbiology (UK), 2004, vol. 150, no. 10, pp. 3197–2308.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Biology, Moscow State University, Vorob’evy gory, Moscow, 119992, Russia

    T. S. Kalebina, T. A. Plotnikova, E. V. Karpova & I. S. Kulaev

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  1. T. S. Kalebina
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  2. T. A. Plotnikova
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  3. E. V. Karpova
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  4. I. S. Kulaev
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Correspondence to T. S. Kalebina.

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Original Russian Text © T.S. Kalebina, T.A. Plotnikova, E.V. Karpova, I.S. Kulaev, 2006, published in Mikrobiologiya, 2006, Vol. 75, No. 5, pp. 717–719.

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Kalebina, T.S., Plotnikova, T.A., Karpova, E.V. et al. A new phenotypic manifestation of deletion of the BGL2 gene encoding the cell-wall glucanotransferase Bgl2p in the yeast Saccharomyces cerevisiae . Microbiology 75, 625–626 (2006). https://doi.org/10.1134/S0026261706050146

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  • DOI: https://doi.org/10.1134/S0026261706050146

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