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Thiobacillus sajanensis sp. nov., a new obligately autotrophic sulfur-oxidizing bacterium isolated from Khito-Gol hydrogen-sulfide springs, Buryatia

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Abstract

Four strains of rod-shaped gram-negative sulfur-oxidizing bacteria were isolated from Khoito-Gol hydrogen-sulfide springs in the eastern Sayan Mountains (Buryatia). The cells of the new isolates were motile by means of a single polar flagellum. The strains were obligately chemolithoautotrophic aerobes that oxidized thiosulfate (with the production of sulfur and sulfates) and hydrogen sulfide. They grew in a pH range of 6.8–9.5, with an optimum at pH 9.3 and in a temperature range of 5–39°C, with an optimum at 28–32°C. The cells contained ubiquinone Q-8. The DNA G+C content of the new strains was 62.3–64.2 mol %. According to the results of analysis of their 16S rRNA genes, the isolates belong to the genus Thiobacillus within the subclass Betaproteobacteria. However, the similarity level of nucleotide sequences of the 16S rRNA genes was insufficient to assign the isolates to known species of this genus. The affiliation to the genus Thiobacillus was confirmed by DNA-DNA hybridization of the isolates with the type strain of the type species of the genus Thiobacillus, T. thioparus DSM 505T (= ATCC 8158T). Despite the phenotypic similarity, the hybridization level was as low as 21–29%. In addition, considerable differences were revealed in the structure of the genes encoding RuBPC, the key enzyme of autotrophic CO2 assimilation, between the known Thiobacillus species and the new isolates. Based on molecular-biological features and certain phenotypic distinctions, the new isolates were assigned to a new Thiobacillus species, T. sajanensis sp. nov., with the type strain 4HGT (= VKM B-2365T).

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References

  1. Kelly, D.P. and Wood, A.P., Reclassification of Some Species of Thiobacillus to the Newly Designated Genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov., and Thermithiobacillus gen. nov., Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 511–516.

    PubMed  Google Scholar 

  2. Kelly, D.P, Wood, A.P, and Stackebrandt, E., Genus Thiobacillus Beijerinck 1904, 597AL, Bergey’s Manual of Systematics Bacteriology, 2nd ed., Brenner, D.J. et al., Eds., vol. 2, part C, Baltimore: Williams & Wilkins, 2005, pp. 764–769.

    Google Scholar 

  3. Hernandez, J.M., Baker, S.H., Lorbach, S.C., Shively, J.M., and Tabita, F.R., Deduced Amino Acid Sequence, Functional Expression, and Unique Enzymatic Properties of the Form I and Form II Ribulose Bisphosphate Carboxylase/Oxygenase from the Chemoautotrophic Bacterium Thiobacillus denitrificans, J. Bacteriol., 1996, vol. 178, no. 2, pp. 347–356.

    PubMed  CAS  Google Scholar 

  4. Danilova, E.V., Structural-Functional Organization of the Microbial Community of the Mineral Springs of Eastern Sayan Mountains, Cand. Sci. (Biol.) Dissertation, Ulan-Ude: Inst. Gen. Exp. Biol., Siberian Division, Russ. Acad. Sci., 2001.

    Google Scholar 

  5. Reznikov, A.A., Mulikovskaya, E.P., and Sokolov, I.Yu., Metody analiza prirodnykh vod (Methods for Analysis of Natural Waters), Moscow: Gosgeoltekhizdat, 1970.

    Google Scholar 

  6. Pfennig, N. and Lippert, R.D., Uber das Vitamin B12 Bedurfnis phototropher Schwefelbakterien, Arch. Microbiol., 1966, vol. 55, no. 1, pp. 245–259.

    CAS  Google Scholar 

  7. Ryter, A. and Kellenberger, E., Etude u microscope electronique de plasmas contennant de l’acide desoxyribonucleique, Z. Naturforschung, 1958, vol. 13, no. 9, pp. 597–605.

    Google Scholar 

  8. Reynolds, E.S., The Use of Lead Citrate at High pH as an Electron-Opaque Stain in Electron Microscopy, J. Cell Biol., 1963, vol. 17, no. 1, pp. 208–213.

    Article  PubMed  CAS  Google Scholar 

  9. Bradford, M.H., A Rapid and Sensitive Method for Quantitation of Micrograms Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  PubMed  CAS  Google Scholar 

  10. Carret, R.H. and Nason, A., Further Purification and Properties of Neurospora Nitrate Reductase, J. Biol. Chem., 1969, vol. 244, pp. 2870–2882.

    Google Scholar 

  11. Karavaiko, G.I., Bogdanova, T.I., Tourova, T.P., Kondrat’eva, T.F., Tsaplina, I.A., Egorova, M.A., Krasilnikova, E.N., and Zakharchuk, L.M., Reclassification of “Sulfobacillus thermosulfidooxidans subsp. thermotolerans” Strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and Emended Description of the Genus Alicyclobacillus, Int. J. Syst. Evol. Microbiol, 2005, vol. 55, pp. 941–947.

    Article  PubMed  CAS  Google Scholar 

  12. Collins, N.D., Analysis of Isoprenoid Quinones, Methods Microbiol., 1985, vol. 18, pp. 329–363.

    Article  CAS  Google Scholar 

  13. Surkov, A.V., Dubinina, G.A., Lysenko, A.M., Glocker, F.O., and Kuever, J., Dethiosulfovibrio russensis sp. nov., Dethiosulfovibrio marinus sp. nov. and Dethiosulfovibrio acidaminovorans sp. nov., Novel Anaerobic, Thiosulfate-and Sulfur-Reducing Bacteria Isolated from “Thiodendron” Sulfur Mats in Different Saline Environments, Int. J. Syst. Evol. Microbiol., 2001, vol. 51, pp. 327–337.

    PubMed  CAS  Google Scholar 

  14. Ushakova, N.A., Belov, L.P., Varshavski, A.A., Kozlova, A.A., Kolganova, T.V., Boulygina, E.S., and Tourova, T.P., Cellulose Decomposition under Nitrogen Deficiency by Bacteria Isolated from the Intestines of Phytophagous Vertebrates, Mikrobiologiya, 2003, vol. 72, no. 3, pp. 400–406 [Microbiology (Engl. Transl.), vol. 72, no. 3, pp. 356–362].

    CAS  Google Scholar 

  15. Pivovarova, T.A. and Karavaiko, G.I., Submicroscopic Organization and Functions of Certain Structures of Thiobacillus neapolitanus, Mikrobiologiya, 1973, vol. 42, no. 4, pp. 672–676.

    CAS  Google Scholar 

  16. Kelly, D.P. and Wood, A.P., Confirmation of Thiobacillus denitrificans as a Species of the Genus Thiobacillus, in the β-Sublcass of the Proteobacteria, with Strain NCIMB 9548 as the Type Strain, Int. J. Syst Evol. Microbiol., 2000, vol. 50, pp. 547–550.

    PubMed  Google Scholar 

  17. Spiridonova, E.M., Berg, I.A., Kolganova, T.V., Ivanovsky, R.N., Kuznetsov, B.B., and Tourova, T.P., An Oligonucleotide Primer System for Amplification of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Genes of Bacteria of Various Taxonomic Groups, Mikrobiologiya, 2004, vol. 73, no. 3, pp. 377–387 [Microbiology (Engl. Transl.), vol. 73, no. 3, pp. 316–325].

    CAS  Google Scholar 

  18. Watson, G.M. and Tabita, F.R., Microbial Ribulose-1.5-Bisphosphate Carboxylase/Oxygenase: A Molecule for Phylogenetic and Enzymological Investigation, FEMS Microbiol. Lett., 1997, vol. 146, pp. 13–22.

    Article  PubMed  CAS  Google Scholar 

  19. Wood, A.P. and Kelly, D.P., Isolation and Physiological Characterization of Thiobacillus aquaesulis sp. nov., a Novel Facultatively Autotrophic Moderate Thermophile, Arch. Microbiol., 1988, vol. 149, no. 4, pp. 339–343.

    Article  CAS  Google Scholar 

  20. Dubinina, G.A., Mechanisms of Colorless Sulfur-Oxidizing Bacteria Adaptation to Their Habitat, Tr. Inst. Mikrobiol., Moscow: Nauka, 2004, vol.12, pp. 126–148.

    Google Scholar 

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    N. M. Dul’tseva, T. P. Tourova & V. M. Gorlenko

  2. Moscow State University, Vorob’evy gory, Moscow, 119899, Russia

    E. M. Spiridonova

  3. Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 1, Moscow, 117312, Russia

    T. V. Kolganova

  4. Bakulev Research Center of Cardiovascular Surgery, Russian Academy of Medical Sciences, Moscow, Russia

    G. A. Osipov

Authors
  1. N. M. Dul’tseva
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  2. T. P. Tourova
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  3. E. M. Spiridonova
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  4. T. V. Kolganova
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  5. G. A. Osipov
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  6. V. M. Gorlenko
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Correspondence to N. M. Dul’tseva.

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Original Russian Text © N.M. Dul’tseva, T.P. Tourova, E.M. Spiridonova, T.V. Kolganova, G.A. Osipov, V.M. Gorlenko, 2006, published in Mikrobiologiya, 2006, Vol. 75, No. 5, pp. 670–681.

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Dul’tseva, N.M., Tourova, T.P., Spiridonova, E.M. et al. Thiobacillus sajanensis sp. nov., a new obligately autotrophic sulfur-oxidizing bacterium isolated from Khito-Gol hydrogen-sulfide springs, Buryatia. Microbiology 75, 582–592 (2006). https://doi.org/10.1134/S0026261706050080

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  • DOI: https://doi.org/10.1134/S0026261706050080

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