Abstract
We established that chemical analogues of alkylhydroxybenzenes (AHB), belonging to alkylresorcinols and functioning as microbial autoregulatory d1 factors, enhance the UV resistance of various DNA molecules of different origin and conformation. These include the linear DNA of the λ phage, bovine spleen DNA, and the DNA of the pUC19 plasmid that is composed of a number of annular (supercoiled and relaxed) and linearized molecules. Irradiating DNA with UV light (λ = 254 nm) in the presence of methylresorcinol (MR) or hexylresorcinol (HR) results in comparatively insignificant DNA destruction as evidenced by our data on the electrophoretic mobility pattern in agarose gel. Using the linear HindIII restricts of the λ phage DNA, we revealed that the protective effect of AHB varies depending on their chemical structure (it is more manifest with HR than MR) and the concentration. Importantly, the effect of HR on bovine spleen DNA was based on its protective activity and manifested itself after a long incubation period. Studies using the pUC19 plasmid demonstrated that AHB, apart from increasing the resistance of linearized DNA molecules to UV irradiation, prevented both the supercoiled annular-supercoiled relaxed and the supercoiled relaxed-linearized transitions. The possible mechanisms of the UV-protective effect of AHB on DNA and their contributions to the resistance of dormant microbial forms to environmental factors are discussed.
Similar content being viewed by others
References
Gromov, B.V. and Pavlenko, G.V., Ekologiya Bakterii (Ecology of Bacteria), Leningrad: Leningr. Univ., 1989.
Rubin, A.B., Biofizika: Biofizika kletochnykh protsessov (Biophysics: 2. Biophysics of Cell Processes), Moscow: Vysshaya Shkola, 1987.
Fraikin, G.Ya., Strakhovskaya, M.G., and Rubin, A.B., Light-Induced Processes of Cell Protection from Photodamage, Biokhimiya, 2000, vol. 65, no. 6, pp. 865–875 [Biochemistry (Engl. Transl., vol. 65, no. 6, pp. 737–746].
Pospisilova, S. and Kypr, J., UV Light-Induced Duplex-To-Duplex Crosslinking of DNA Molecules in Aqueous Ethanol Solutions, Photochem. Photobiol., 1998, vol. 67, no. 4, pp. 386–390.
Demkina, E.V., Soina, V.S., El’-Registan, G.I., and Zvyagintsev, D.G., Reproductive Resting Forms of Arthrobacter globiformis, Mikrobiologiya, 2000, vol. 69, no. 3, pp. 377–382, [Microbiology (Engl. Transl.), vol. 69, no. 3, pp. 309–313].
Mulyukin, A.L, Soina, V.S, Demkina, E.V, Kozlova, A.N., Suzina, N.E, Dmitriev, V.V, Duda, V.I, and El-Registan, G.I, Formation of Resting Cells by Non-Spore-Froming Microorganisms as a Strategy of Long-Term Survival in the Environment, Hoover, R.B., Rozanov, A.Yu., and Lipps, J.H., Eds., Proceedings of SPIE. V. 4939. Instruments, Methods, and Missions for Astrobiology VI, Bellingham: SPIE, 2003, pp. 208–218.
Davydova, O.K., Deryabin, D.G., Nikiyan, A.N., and El’-Registan, G.I., Mechanisms of Interaction between DNA and Chemical Analogues of Microbial Anabiosis Autoinducers, Mikrobiologiya, 2005, vol. 74, no. 5, pp. 614–625 [Microbiology (Engl. Transl.), vol. 74, no. 5, pp. 533–541].
Kolpakov, A.I., Il’inskaya, O.N., Bespalov, M.M., Kupriyanova-Ashina, F.G., Gal’chenko, V.F., Kurganov, B.I., and El’-Registan, G.I., Stabilization of Enzymes by Dormancy Autoinducers as a Possible Mechanism of Resistance of Resting Microbial Forms, Mikrobiologiya, 2000, vol. 69, no. 2, pp. 224–230 [Microbiology (Engl. Transl.), vol. 69, no. 2, pp.180–185].
Stepanenko, I.Yu., Mulyukin, A.L., Kozlova, A.N., Nikolaev, Yu.A., and El’-Registan, G.I., The Role of Alkylhydroxybenzenes in the Adaptation of Micrococcus luteus to Heat Shock, 2005, vol. 74, no. 1, pp. 26–33 [Microbiology (Engl. Transl.), vol. 74, no. 1, pp. 20–26].
Stepanenko, I.Yu., Strakhovskaya, M.G., Belenikina, N.S., Nikolaev, Yu.A., Mulyukin, A.L., Kozlova, A.N., Revina, A.A., and El’-Registan, G.I., Protection of Saccharomyces cerevisiae against Oxidative and Radiation-Caused Damage by Alkylhydroxybenzenes, Mikrobiologiya, 2004, vol. 73, no. 2, pp. 204–210 [Microbiology (Engl. Transl.), vol. 73, no. 2, pp.163–169].
El-Registan, G.I., Mulyukin, A.L., Nikolaev, Yu.A., Stepanenko, I.Yu., Kozlova, A.N., Martirosova, E.I., Shanenko, E.F., Strakhovskaya, M.G., and Revina, A.A., The Role of Microbial Low-Molecular-Weight Autoregulatory Factors (Alkylhydroxybenzenes) in Resistance of Microorganisms To Radiation and Heat Shock, J. Adv. Space Res, 2005, vol. 36, no. 9, pp. 1718–1728.
Scannell, R.T., Barr, J.R., Murty, V.S., Reddy, K.S., and Hecht, S.M., DNA Strand Scission by Naturally Occurring 5-Alkylresorcinols, J. Am. Chem. Soc., 1998, vol. 110, pp. 3650–3651.
Martirosova, E.I., Karpekina, T.A., and El’-Registan, G.I., Enzyme Modification by Natural Chemical Chaperons of Microorganisms, Mikrobiologiya, 2004, vol. 73, no. 5, pp. 708–715 [Microbiology (Engl. Transl.), vol. 73, no. 5, pp. 609–615].
Bespalov, M.M., Kolpakov, A.I., Loiko, N.G., Doroshenko, E.V., Mulyukin, A.L., Kozlova, A.N., Varlamova, E.A., Kurganov, B.I., and El’-Registan, G.I., The Role of Microbial Dormancy Autoinducers in Metabolism Blockade, Mikrobiologiya, 2000, vol. 69, no. 2, pp. 217–223 [Microbiology (Engl. Transl.), vol. 69, no. 2, pp.174–179].
Kozubek, A. and Tyman, J.H.P., Resorcinolic Lipids, the Natural Non-Isoprenoid Phenolic Amphiphiles and Their Biological Activity, Chem. Rev., 1999, vol. 99, no. 1, pp. 1–31.
Revina, A.A., Larionov, O.G., Kochetova, M.V., Lutsik, T.K., and El’-Registan, G.I., Spectrophotometric and Chromatographic Study of the Products of Radiolysis of Aerated Alkylresorcinol Aqueous Solutions, Izvestiya AN, Seriya Khimicheskaya, 2003, vol. 11, pp. 2257–2263.
Davydova, O.K., Nikiyan, A.N., and Deryabin, D.G., Formation of Ordered Supramolecular DNA Structures in Aqueous Solutions in the Presence of alkylresorcinols, Vestnik OGU, 2005, no. 1, pp. 174–177.
Ivanov, V.I. and Minchenkova, L.E., A Form of DNA: in Search of the Biological Role, Mol. Biol., 1994, vol. 28, pp. 1258–1271.
Vagel, A. and Roo, E., Alkylresorcinols-Rare Chemicals Available in Bulk, Innov. Pharm. Tech, 2004, pp. 94–95.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.K. Davydova, D.G. Deryabin, G.I. El’-Registan, 2006, published in Mikrobiologiya, 2006, Vol. 75, No. 5, pp. 654–621.
Rights and permissions
About this article
Cite this article
Davydova, O.K., Deryabin, D.G. & El’-Registan, G.I. Influence of chemical analogues of microbial autoregulators on the sensitivity of DNA to UV radiation. Microbiology 75, 568–574 (2006). https://doi.org/10.1134/S0026261706050067
Received:
Issue Date:
DOI: https://doi.org/10.1134/S0026261706050067