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Features of Reactions to Stress in Rats with Passive-Defense Behavior after Light Desynchronosis and Physical Activity

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Abstract

An assessment was made of hematological parameters of peripheral blood and structural and functional changes in the adrenal glands in stress-unresistant rats (passive-defensive type of behavior in the open field test) after exposure to light deprivation and physical activity during the spring equinox. The experiment showed that light deprivation for 10 days reduces the total number of leukocytes, the absolute content of monocytes, granulocytes, lymphocytes and the level of corticosterone in the peripheral blood compared to the intact group, that was on a natural lighting regime. Histological analysis of the adrenal glands of this group showed, that in the zona fasciculata under such conditions there was nuclear hypertrophy, an increase in the nuclear-cytoplasmic ratio of adrenocorticocytes and a decrease in the size of the zona glomerulosa of the adrenal cortex of rats in relation to the intact group. Physical activity in the form of forced swimming until complete fatigue for 5 days in a row in natural light in stress-unresistant rats did not change the parameters of the peripheral blood of animals, however it led to an increase in the area of the cytoplasm, the nuclear-cytoplasmic ratio, as well as the formation of nuclear hypertrophy of adrenocorticocytes in the fascicle adrenal zones, which indicated the preparation of cells for increased synthetic activity. Keeping rats for 10 days in complete darkness before forced swimming every day for 5 days, on the one hand, formed a hypoxic state and exhaustion of the adrenal glands, on the other hand, stabilized the leukocyte pool of peripheral blood compared to similar indicators in intact rats.

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REFERENCES

  1. Umryukhin AE, Sudakov KV (2013) Behavior in the open field and the state of the adrenal glands and thymus in rats after stress loads with increasing levels of autoantibodies to dopamine and glutamate in the blood. Bull Exp Biol Med 155: 532–535. (In Russ).

    Google Scholar 

  2. Kotelnikova SV, Kotelnikov AV, Temply DL (2014) Influence of the illumination mode on the intensity of lipid peroxidation in normal and cadmium intoxication. Natl Sci 3: 55–62.https://doi.org/10.17116/jnevro201811811182

    Article  Google Scholar 

  3. Mamylina NV, Pavlova VI (2014) Physiological aspects of behavioral activity of animals under emotional stress. Pica, Chelyabinsk. (In Russ).

    Google Scholar 

  4. Ismailova HY, Agaev TM, Semenova TP (2007) Individual features of behavior. Nurlan, Baku. (In Russ).

    Google Scholar 

  5. Benus RF, Bohus B, Koolhaas JM, van Oortmerssen JM (1991) Behavioural differences between artificially selected aggressive and nonaggressive mice: response to apomorphine. Behav Brain Res 43: 203–208.https://doi.org/10.1016/s0166-4328(05)80072-5

    Article  CAS  PubMed  Google Scholar 

  6. Driscoll P, Demek M, Angio D, Claustre Y, Scatton B (eds) (1990) Genetically-based model for divergent stress responses: behavioral, neurochemical and hormonal aspects. Verlag Paul Parey, Hamburg, 97–107.https://doi.org/10.1080/1025389031000111320

    Book  Google Scholar 

  7. Simonov PV (1987) Motivated brain. Science, M. (In Russ).

    Google Scholar 

  8. Sel’e G (1960) Essays on the Adaptation Syndrome. Medgiz, M. (In Russ).

    Google Scholar 

  9. Kolesnikova LA, Os’kina IN (2003) Blood corticosterone concentration and differential leukocyte count in stressed Norway rats with different modes of behavior. Izv Akad Nauk Ser Biol 1: 88–92. (In Russ).

    Google Scholar 

  10. Tomova TA, Zamoschina TA, Svetlik MV, Sedokova ML, Gostyukhina AA, Fatyushina AM (2020) Gly-Pro and adaptive reactions under multicomponent stress. Russ J Physiol 106: 231–242.https://doi.org/10.31857/S0869813920020107

    Article  Google Scholar 

  11. Kirillov OI, Khasina EI, Durkin VB (2003) Effect of stress on postnatal growth of body weight and adrenal glands of rats. Ontogenesis 34(5): 371–376. (In Russ).

    CAS  Google Scholar 

  12. Buresh Y (1991) Methods and basic experiments on the study of the brain and behavior. Higher School, M. (In Russ).

    Google Scholar 

  13. Gostyukhina AA, Zamoshchina TA, Svetlik MV, Zhukova OB, Zaitsev KV, Abdulkina NG (2016) Behavioral activity of rats in the “open field” after light or dark deprivation and physical fatigue. Bull Siber Med 15(3): 16–23.https://doi.org/10.20538/1682-0363-2016-3-16-23

    Article  Google Scholar 

  14. Gostyukhina AA, Zamoshchina TA, Prokopova AV, Zaitsev KV (2022) Individual typological features of laboratory rats’ response to multicomponent stress Modern Issues Biomed 6: 47–55.https://doi.org/10.51871/2588-0500_2022_06_02_5

  15. Zamoshchina T (2000) Lithium oxybutyrate and the rhythmic structure of active search behavior and body temperature of rats under constant lighting conditions. Exp Clin Pharmacol 63(2): 12–15. (In Russ).

    CAS  Google Scholar 

  16. Patent for invention No. 2617206/ 21.04.2017. Byul No 12. Gostyukhina AA, Zaitsev KV, Zamoshchina TA, Svetlik MV, Zhukova OB, Abdulkina NG, Zaitsev AA, Vorobyev VA A method for modeling physical fatigue in rats under conditions of desynchronosis. (In Russ).

  17. Exbrayat JM (2013) Classical methods of visualization. Histochemical and cytochemical methods of visualization. CRC Press Taylor and Francis Group, Boca Raton; London; New York, 3–58.https://doi.org/10.1201/B14967

    Book  Google Scholar 

  18. RF GOST R-53434-2009 Principles of good laboratory practice. M. Standartinform. 2010. (In Russ).

  19. Medic VA, Simonov CA (2000) Statistics in medicine and biology. Medicine, M. (In Russ).

    Google Scholar 

  20. Glazachev OS, Kryzhanovskaya SYu (2021) In search of a structural and functional trace of the effects of cross-adaptation: problems of translational physiology. Human Physiol 47(6): 125–133.https://doi.org/10.31857/s0131164621050040

    Article  Google Scholar 

  21. Meerson FZ, Pshennikova MG (1998) Adaptation to stressful situations and physical activity. Medicine, M. (In Russ).

    Google Scholar 

  22. Rumyantseva ER, Gorulev PS (2005) Sports training of weightlifters. Mechanisms of adaptation. Theory and Practice of Physical Culture, M. (In Russ).

    Google Scholar 

  23. Meerson FZ (1993) Adaptive medicine: the concept of long–term adaptation. Delo, M. (In Russ).

    Google Scholar 

  24. Gostyukhina AA, Zamoshchina TA, Zaitsev KV, Gutor SS, Zhukova OB, Svetlik MV, Abdulkina NG, Zaitsev AA (2018) Adaptive reactions of rats after light desynchronosis and physical. Bull Siber Med 17 (3): 22–34.https://doi.org/10.51871/2588-0500_2022_06_02_5

    Article  Google Scholar 

  25. Tomova TA, Zamoshchina TA, Fedorutseva EYu, Svetlik MV (2017) Seasonal effects of gly-pro peptide on the secretory function of the stomach in rats with different reactivity of the central nervous system. Exp Clin Gastroenterol 6(142): 66–71.https://doi.org/10.51871/2588-0500_2022_06_02_5

    Article  Google Scholar 

  26. Voltarelli FF, Gobatto CA, R de Mello MA (2002) Determination of anaerobic threshold in rats using the lactate minimum test. Braz J Boil Res 35(11): 1389–1394.https://doi.org/10.1016/j.ynstr.2014.10.001

    Article  CAS  Google Scholar 

  27. Gostyukhina AA, Zaitsev KV, Zamoshchina TA, Zhukova OB, Gutor SS, Svetlik MV, Abdulkina NG (2016) Seasonal features of corticosterone content in blood serum of rats after physical overwork in conditions of desynchronosis. Russ J Physiol 102(1): 50–55. (In Russ).

    CAS  Google Scholar 

  28. Gostyukhina AA, Zamoshchina TA, Prokopova AV, Zajtsev KV (2022) Individual and typological features of the response of laboratory rats to multi-component stress. Modern Issues Biomed 6(2): 47–55.https://doi.org/10.51871/2588-0500_2022_06_02_5

    Article  Google Scholar 

  29. Kondashevskaya MV (2019) Comparative analysis of hormonal and behavioral changes in models of post-traumatic stress disorders and acute stress. Russ J Physiol 105(7): 879–887.https://doi.org/10.1134/S0869813919070045

    Article  Google Scholar 

  30. Yeltokova M, Ulyanova O, Askarov M, Chernyshova A, Kozina L (2019) Integral hematologic indices in the evaluation of the immunologic reactivity of the organism in a patient with complication of type 1 diabetes mellitus: a case of diabetic retinopathy after autologous mesenchymal stem cell transplant. Exp Clin Transplant 17(1): 234–235.https://doi.org/10.6002/ect.MESOT2018.P9

    Article  PubMed  Google Scholar 

  31. Dhabhar FS, Malarkey WB, Neri E, McEwen BS (2012) Stress-induced redistribution of immune cells-from barracks to boulevards to battlefields: a tale of three hormones-curt richter award winner. Psychoneuroendocrinology 37(9): 1345–1368.https://doi.org/10.1016/j.psyneuen.2012.05.008

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Tomova TA, Zamoshchina TA, Prosekina EYu, Svetlik MV (2015) The effect of carbacholine and glycylproline (GLY-PRO) on the secretory function of the stomach depending on the reactivity of the central nervous system in rats. Exp Clin Pharmacol 78(3): 13–16.https://doi.org/10.30906/0869-2092-2015-78-3-13-16

    Article  CAS  Google Scholar 

  33. Reul JMH, Collins A, Saliba RS, Mifsud KR, Carter SD, Gutierrez-Mecinas M, Qian X, Linthorst ACE (2015) Glucocorticoids, epigenetic control and stress resilience. Neurobiol Stress 1: 44–59.https://doi.org/10.1016/j.neuchi.2014.06.003

    Article  PubMed  Google Scholar 

  34. Polina YuV, Naumova LI, Shishkina TA, Rodzaevskaya EB (2020) Morpho-functional changes the adrenal glands of rats under the conditions of an experimental model of immobilization stress. Forcipe 3(S2): 32–35. (In Russ).

    Google Scholar 

  35. Alyabyev FV, Parfiryeva AM, Logvinov SV (2007) Morphometric parameters of rat adrenals in the dynamics of general hypothermia. Morphology 162(6): 52–56. (In Russ).

    Google Scholar 

  36. Antonov EV, Markel’ AL, Yakobson GS (2011) Aldosterone and stress-dependent arterial hypertension. Bull Exp Biol Med 152(8): 148–151.https://doi.org/10.1007/s10517-011-1484-8

    Article  CAS  Google Scholar 

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Funding

This work was funded by the State Assignment of the Federal Medical-Biological Agency. No additional grants were received to conduct or supervise this particular study.

Author information

Authors and Affiliations

  1. Federal Scientific and Clinical Center of Medical Rehabilitation and Balneology of the Federal Medical and Biological Agency of Russia, Moscow oblast, Solnechnogorsk district, Goluboe village, Russia

    A. A. Gostyukhina & K. V. Zaitsev

  2. National Research Tomsk State University, Tomsk, Russia

    T. A. Tomova, A. A. Gostyukhina, T. A. Zamoshchina, M. V. Svetlik, N. D. Blazhko, E. V. Medvedeva, S. A. Ogorodnikov & V. V. Yartsev

  3. Siberian State Medical University SSMU, Tomsk, Russia

    T. A. Zamoshchina, M. V. Svetlik & V. V. Yartsev

  4. Tomsk State Pedagogical University, Tomsk, Russia

    T. A. Tomova

Authors
  1. T. A. Tomova
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  2. A. A. Gostyukhina
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  3. T. A. Zamoshchina
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  4. M. V. Svetlik
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  5. N. D. Blazhko
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  6. E. V. Medvedeva
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  7. S. A. Ogorodnikov
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  8. V. V. Yartsev
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  9. K. V. Zaitsev
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Contributions

T.A.T.—analyzing and interpreting data, writing the manuscript of the article. A.A.G.—conducting experimental and analytical part of the research, analyzing and interpreting data, writing the manuscript of the article. T.A.Z.—analyzing and interpreting data, writing the manuscript of the article. M.V.S.—statistical processing of the results. K.V.Z.—conceptualization and design. V.V.Ya.—analyzing and interpreting data on histological analysis. N.D.B.—carrying out the experimental part of the study, design of the article according to the rules of the journal. E.V.M.—conducting histologic analysis, statistical processing of the results obtained by morphometry and cytometry of the adrenal glands. S.A.O.—histologic analysis, statistical processing of the results obtained by morphometry and cytometry of the adrenal glands.

Corresponding author

Correspondence to A. A. Gostyukhina.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

Experiments with animals were conducted in accordance with international recommendations for biomedical research with laboratory animals and were approved by the Local Ethical Committee of the Tomsk Research Institute of Balneology and Physiotherapy, a branch of the Federal State Budgetary Institution Federal Scientific and Clinical Center for Medical Rehabilitation and Balneology of the Federal Medical-Biological Agency, Russia (Minutes no. 3 of March 22, 2012).

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The authors of this work declare that they have no conflicts of interest.

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Translated by A. Dyomina

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Tomova, T.A., Gostyukhina, A.A., Zamoshchina, T.A. et al. Features of Reactions to Stress in Rats with Passive-Defense Behavior after Light Desynchronosis and Physical Activity. J Evol Biochem Phys 60, 420–431 (2024). https://doi.org/10.1134/S0022093024010320

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