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Age-Related Effect of Cholecystokinin on Impulse Activity of Neurons in the Rat Hypothalamic Dorsomedial and Ventromedial Nuclei

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Abstract

The aim of this work was to analyze background impulse activity, as well as the firing evoked by the intravenous administration of a sulfated cholecystokinin (CCK) octapeptide and its antagonist proglumide, in neurons of the hypothalamic dorsomedial (DMN) and ventromedial (VMN) nuclei in urethane-anesthetized young (2–3 months), adult (12 months), and aged (24 months) male Wistar rats. The mean background firing rate in young rats significantly decreased after CCK administration from 1.5 ± 0.4 to 0.2 ± 0.1 spikes/s in the DMN and from 2.0 ± 0.4 to 0.9 ± 0.2 spikes/s in the VMN. CCK and proglumide co-administration evoked no changes in firing patterns in this age group. In adult and aged rats, the mean background firing rate in DMN and VMN neurons was lower compared to young rats, and did not change significantly in response to CCK and proglumide exposure. In the DMN and VMN, most neurons were inhibited by CCK, while in the DMN, the percentage of CCK-inhibited neurons was higher compared to the VMN. In young animals, the DMN lacked CCK-activated neurons that were observed in adult and aged animals.

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Funding

This work was supported by the Russian Science Foundation (grant no. 19-15-00039).

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Authors and Affiliations

  1. Yaroslavl State Medical University, Yaroslavl, Russia

    A. A. Spirichev, K. Yu. Moiseev, P. A. Anfimova, G. A. Tarasova & P. M. Masliukov

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  1. A. A. Spirichev
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  2. K. Yu. Moiseev
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  3. P. A. Anfimova
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  4. G. A. Tarasova
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  5. P. M. Masliukov
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Contributions

Conceptualization and experimental design—P.M.M., data collection—A.A.S., K.Yu.M., P.A.A., data processing—A.A.S., K.Yu.M., P.A.A., G.A.T., P.M.M., writing and editing the manuscript—P.M.M.

Corresponding author

Correspondence to P. M. Masliukov.

Ethics declarations

COMPLIANCE WITH ETHICAL STANDARDS

All applicable international, national and/or institutional principles of animal care and use were observed. All experimental procedures performed with the involvement of animals complied with the ethical standards approved by the legal acts of the Russian Federation, the principles of the Basel Declaration, and the recommendations of the Ethics Committee at Yaroslavl State Medical University (Meeting minutes No. 60 of 16.02.2023).

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

Additional information

Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 8, pp. 1056–1067https://doi.org/10.31857/S0869813923080101.

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Spirichev, A.A., Moiseev, K.Y., Anfimova, P.A. et al. Age-Related Effect of Cholecystokinin on Impulse Activity of Neurons in the Rat Hypothalamic Dorsomedial and Ventromedial Nuclei. J Evol Biochem Phys 59, 1382–1391 (2023). https://doi.org/10.1134/S0022093023040294

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