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Reaction of Rat Renal Medullary Laminin to Prolonged Dehydration

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Abstract

Laminins are the major water-soluble glycoproteins in the basement membrane of epithelial tissue. The laminin content of the renal parenchyma was studied in rats that were fed a 60-h alternative water diet with either excessive water intake or a complete lack of drinking water. It was shown that osmotic concentration of the urine, which develops due to water deprivation, is accompanied by a quantitative change in the laminin composition. It was found that the level of the laminin β chain increases in the renal medulla, but not in the renal cortex, of dehydrated rats. This effect occurs only in WAG rats with a normally expressed vasopressin gene and is absent in mutant Brattleboro rats unable to synthesize this hormone. Based on the initial and new data, it is assumed that laminin, as a key regulatory element in the basement membrane of the tubular epithelium, is involved in the adaptive response of the renal urine concentrating system to prolonged dehydration. Hyperhydration does not affect laminin levels in the renal parenchyma.

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Funding

The work was implemented within the framework of the budget project No. FWNR-2022-0021 (Gene funds of the population of Siberia, genetic markers of human diseases and the molecular basis for the formation of pathological processes).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia

    I. I. Khegay

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  1. I. I. Khegay
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Correspondence to I. I. Khegay.

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COMPLIANCE WITH ETHICAL STANDARDS

All applicable international and national guidelines for the care and use of animals were observed. All experimental procedures involving animals complied with the ethical standards approved by the legal acts of the Russian Federation, the principles of the Basel Declaration and the recommendations of the Bioethics Committee at Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences (meeting minutes No. 98/1 of 05.11.2021).

CONFLICT OF INTEREST

The author declares that he has no conflict of interest.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 7, pp. 946–953https://doi.org/10.31857/S0869813923070051.

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Khegay, I.I. Reaction of Rat Renal Medullary Laminin to Prolonged Dehydration. J Evol Biochem Phys 59, 1297–1302 (2023). https://doi.org/10.1134/S0022093023040221

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