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Genome Instability of Hippocampal and Bone Marrow Cells in Male Mice Exposed to Immobilization and Female Pheromone Stressor

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Abstract

Different stressors, while affecting the cells of target organs, can lead to genome instability and even disintegration, which can matter in the formation of post-stress disorders. We studied the effect of psycho-emotional stressors (immobilization and the house mouse female pheromone 2,5-dimethylpyrazine) on DNA integrity of hippocampal and bone marrow cells in CD-1, CBA and C3H male mice, using the cytogenetic and immunocytochemical methods (alkaline comet assay, ana-telophase test for mitotic disturbances, γH2AX focus assay). It was shown that both immobilization and 2,5-dimethylpyrazine cause genome damage in the cells of both organs studied. Cell genome destabilization in various organs is considered as an essential stage of stress response development in an attempt of the organism to adapt to extreme environmental exposures.

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ACKNOWLEDGMENTS

The authors are grateful to A.A. Bondarenko and other students and associates of the Genetics and Biotechnology Chair of St. Petersburg State University for their assistance in conducting experiments and involvement is the discussion of the results obtained.

Funding

The work was supported by the Russian Foundation for Basic Research (RFBR) grant no. 16-04-00678.

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Authors and Affiliations

  1. St. Petersburg State University, St. Petersburg, Russia

    V. D. Shcherbinina, B. V. Bakulevskiy, T. S. Glinin & E. V. Daev

  2. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    V. D. Shcherbinina & E. V. Daev

Authors
  1. V. D. Shcherbinina
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  2. B. V. Bakulevskiy
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  3. T. S. Glinin
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  4. E. V. Daev
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Contributions

Conceptualization and experimental design (E.V.D.), data collection (V.D.Sh., B.V.B., T.S.G.), data processing and analysis (V.D.Sh., B.V.B.), writing and editing the manuscript (E.V.D., V.D.Sh.).

Corresponding author

Correspondence to E. V. Daev.

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COMPLIANCE WITH ETHICAL STANDARDS

All applicable international, national and/or institutional guidelines for the care and use of animals have been followed. All experimental procedures with the involvement of animals complied with the ethical standards approved by the legal acts of the Russian Federation, the principles of the Basel Declaration and the recommendations of the St. Petersburg University Ethics Committee for working with laboratory animals (conclusion No. 131-03-1 dated 06/01/2017).

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest related to the publication of this article.

Additional information

Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 7, pp. 844–861https://doi.org/10.31857/S0869813923070129.

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Shcherbinina, V.D., Bakulevskiy, B.V., Glinin, T.S. et al. Genome Instability of Hippocampal and Bone Marrow Cells in Male Mice Exposed to Immobilization and Female Pheromone Stressor. J Evol Biochem Phys 59, 1215–1228 (2023). https://doi.org/10.1134/S0022093023040154

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