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Calcium-Accumulating Ability of Rat Liver Mitochondria in Hypothermia of Various Duration

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Abstract

Calcium is one of the most important intracellular messengers that regulate physiological and biochemical processes in the cell. Mitochondria are able to deposit calcium ions and involved in the regulation of calcium signaling. Hypothermic exposures in homeothermic animals can lead to a disruption of this important mitochondrial function and resulting pathological consequences. The aim of this study was to study the effects of moderate (30°C) hypothermia of varying duration on the calcium-accumulating ability of rat liver mitochondria. The experiments were carried out on male rats Wistar. Hypothermia was induced by external cooling of animals in a plexiglas chamber with a cold water jacket. Mitochondria were isolated from the liver of decapitated rats by differential centrifugation. The calcium-accumulating ability of mitochondria was assessed by the kinetics of calcium-induced mitochondrial swelling and their calcium retention capacity (CRC). It was found that during short-term (30-min) moderate (30°C) hypothermia, mitochondrial swelling rate decreases; hypothermia prolongation up to 1 h promotes a further decrease in the swelling rate, and up to 3 h causes its normalization. A positive correlation was found between the rate of mitochondrial calcium-induced swelling and CRC (r = 0.79). Thus, long-term cold exposure in rats activates a number of compensatory-adaptive responses. The decrease in the rate of mitochondrial calcium-induced swelling and CRC at the initial stages of hypothermia may be due to mitochondrial pore formation and is reversible.

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Abbreviations

ROS:

reactive oxygen species

LPO:

lipid peroxidation

P/O ratio:

phosphate/oxygen ratio

MPTP:

mitochondrial permeability transition pore

OPM:

oxidative protein modification

VDAC:

voltage-dependent anion channel

ANT:

adenine nucleotide translocase

CypD:

cyclophilin D

PiC:

mitochondrial phosphate carrier

NADPH:

reduced nicotinamide adenine dinucleotide phosphate

NAD:

reduced nicotinamide adenine dinucleotide

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Funding

This work was supported in part by the State assignment (FZNZ-2020-0002).

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Authors and Affiliations

  1. Dagestan State University, Makhachkala, Republic of Dagestan, Russia

    S. I. Khizrieva, R. A. Khalilov, A. M. Dzhafarova & V. R. Abdullaev

Authors
  1. S. I. Khizrieva
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  2. R. A. Khalilov
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  3. A. M. Dzhafarova
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  4. V. R. Abdullaev
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Contributions

S.I.Kh.—data collection and description; R.A.Kh.—conceptualization and experimental design; A.M.D.—statistical data processing and data interpretation; V.R.A.—critical literature analysis and conclusion generating.

Corresponding author

Correspondence to A. M. Dzhafarova.

Ethics declarations

COMPLIANCE WITH ETHICAL STANDARDS

All applicable international, national and/or institutional principles of care and use of laboratory animals were observed. All experimental procedures that involved animals complied with ethical standards approved by legal acts of the Russian Federation, the principles of the Basel Declaration, the Order of the Russian Federation Ministry of Health no. 199n of 01.04.2016 (Rules of Good Laboratory Practice).

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest related to the publication of this article.

Additional information

Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2023, Vol. 59, No. 4, pp. 310–318https://doi.org/10.31857/S0044452923040046,.

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Khizrieva, S.I., Khalilov, R.A., Dzhafarova, A.M. et al. Calcium-Accumulating Ability of Rat Liver Mitochondria in Hypothermia of Various Duration. J Evol Biochem Phys 59, 1077–1085 (2023). https://doi.org/10.1134/S0022093023040063

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