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Impact of Ozone on the Oxygen Affinity Blood Properties and Prooxidant-Antioxidant Balance under Effect of H2S-Generating System

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Abstract

Hydrogen sulfide belongs to the group of signaling agents called gaseous transmitters and plays an important role in many physiological processes, in particular, in the realization of oxygen-dependent mechanisms. The aim of this study was to evaluate the significance of hydrogen sulfide in the effect of ozone on the oxygen affinity blood properties and the prooxidant-antioxidant balance in vitro experiment. Ozone (6 mg/L concentration) and drugs that affect the synthesis of hydrogen sulfide (propargylglycine, sodium hydrosulfide and its combination with nitroglycerin) were used. The use of propargylglycine, an inhibitor of the synthesis of hydrogen sulfide, leads to a decrease in the effect of ozone on the blood oxygen transport function (decrease in PO2, SO2, P 50real). When sodium hydrosulfide is added, the effect of this gas on these parameters does not increase, but in its combination with nitroglycerin, the effect of ozone on the blood oxygen transport function increases. Propargylglycine does not affect the prooxidant-antioxidant balance under the conditions of the experiment, and donors of hydrogen sulfide and nitrogen monoxide increase the activity of catalase. Propargylglycine under the action of ozone leads to a decrease in the level of nitrate/nitrite, and sodium hydrosulfide increases their concentration. The combination of sodium hydrosulfide and nitroglycerin leads to the accumulation of hydrogen sulfide in the blood plasma.

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Funding

The work was carried out within the framework of the State Scientific Research Program of the Republic of Belarus, Contract no. 30-24/549-21.

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Authors and Affiliations

  1. Grodna State Medical University, Grodna, Belarus

    V. V. Zinchuk & E. S. Biletskaya

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  1. V. V. Zinchuk
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All authors contributed substantially to the conception, research, and preparation of the article materials, and read and approved the final version of the article before publication.

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Correspondence to V. V. Zinchuk.

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COMPLIANCE WITH ETHICAL STANDARDS

All procedures performed in studies involving animals complied with ethical standards approved by legal acts of the Republic of Belarus, the principles of the Basel Declaration, and the recommendations of the Committee on Bioethics and Deontology of the educational institution Grodno State Medical University, Minutes no. 1 of January 14, 2019.

CONFLICT OF INTEREST

The authors declare that they have no conflicts of interest.

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Translated by A. Dyomina

Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 6, pp. 760–770https://doi.org/10.31857/S0869813923060080.

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Zinchuk, V.V., Biletskaya, E.S. Impact of Ozone on the Oxygen Affinity Blood Properties and Prooxidant-Antioxidant Balance under Effect of H2S-Generating System. J Evol Biochem Phys 59, 960–968 (2023). https://doi.org/10.1134/S0022093023030274

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