Abstract
The work was aimed to evaluate the immunophenotype of adipose tissue stem cells isolated from epicardial and perivascular fat depots in patients with coronary heart disease and acquired heart disease. In cell culture obtained from epicardial adipose tissue (EAT) and perivascular adipose tissue (PVAT) (second passage) of patients with both coronary heart disease and acquired heart disease, there was a high (over 90%) expression of membrane proteins characteristic of stem cells. In addition to a major cell population, the two minor were found both in EAT and in PVAT cell cultures: (1) CD90–, CD105+, CD34–/+, CD73+, CD45– (putative endothelial), and (2) CD90+, CD105–, CD34–, CD73–, CD45– (the smallest). Thus, at early stages of cultivation, mesenchymal cells of the stromal vascular fraction isolated from EAT and PVAT express surface markers characteristic of adipose tissue stem cells.
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Funding
The article was prepared within the basic research project No. 0419-2022-0002 entitled “Development of innovative models for management of cardiovascular disease risk factors and comorbidities based on the study of fundamental, clinical, and epidemiological mechanisms and healthcare management techniques in the industrial region of Siberia”.
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Conceptualization (E.G.U., O.V.G.), data collection and analysis (Yu.A.D., E.V.B., O.L.T.), experimental design (E.G.U., Yu.A.D., E.V.B., O.V.G., V.G.M.), taking adipose tissue biopsy samples during surgery (S.M.G.), writing and editing the manuscript (E.G.U.).
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All studies were carried out in accordance with the principles of biomedical ethics, formulated in the Declaration of Helsinki of 1964 and its subsequent updates, and approved by the Bioethics Committee of the Research Institute for Complex Issues of Cardiovascular Diseases (Kemerovo). Each human participant signed a voluntary informed consent to be involved in the study.
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Translated by A. Polyanovsky
Russian Text © The Author(s), 2023, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2023, Vol. 59, No. 3, pp. 215–222https://doi.org/10.31857/S0044452923030099.
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Uchasova, E.G., Dyleva, Y.A., Belik, E.V. et al. Immunophenotype of Mesenchymal Stem Cells Derived from Epicardial and Perivascular Adipose Tissue in Patients with Cardiovascular Diseases. J Evol Biochem Phys 59, 727–734 (2023). https://doi.org/10.1134/S0022093023030079
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DOI: https://doi.org/10.1134/S0022093023030079