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Bilateral Synchronization of Hippocampal Theta Oscillations in vitro

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Abstract

Hippocampal theta oscillations are pivotal for hippocampal functions of spatial navigation, learning and memory. In freely behaving animals, hippocampal theta oscillations display bilateral synchronization. Internally generated oscillations in the theta frequency range have also been described in the intact hippocampal preparation in vitro. However, it remains elusive how theta oscillations are synchronized between the left and right hippocampi. We used a preparation of the intact hippocampi interconnected by the ventral hippocampal commissure, prepared from juvenile and adult mice and rats in vitro. Local field potentials and multiunit activity were recorded using extracellular electrodes from the pyramidal cell layer and stratum radiatum of the left and right hippocampi. Neuronal network activity in the left and right hippocampi was found to be organized in theta oscillations, which strongly modulated the firing of CA1 neurons. Both neuronal activity and field potential theta oscillations demonstrated high levels of bilateral synchronization. Theta oscillations persisted on both sides, but their bilateral synchronization was abolished after surgical transection of the ventral hippocampal commissure. Thus, theta oscillations are synchronized in the left and right hippocampi in vitro, and their bilateral synchronization is provided by the ventral commissural connections.

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Funding

This work was supported by the National Research Agency (Project No. ANR-21-CE16-0005-01 “DevHippo”), and as part of the Priority 2030 research program at Kazan Federal University.

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Authors and Affiliations

  1. Aix-Marseille University, INMED, INSERM, Marseille, France

    I. Khalilov, A. Gainutdinov & R. Khazipov

  2. Laboratory of Neurobiology, Kazan Federal University, Kazan, Russia

    I. Khalilov & R. Khazipov

Authors
  1. I. Khalilov
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  2. A. Gainutdinov
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  3. R. Khazipov
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Contributions

Conceptualization and experimental design (R.Kh.), data collection (I.Kh.), data processing (A.G.), writing and editing the manuscript (R.Kh., A.G., I.Kh.).

Corresponding author

Correspondence to R. Khazipov.

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COMPLIANCE WITH ETHICAL STANDARDS

All procedures performed with the involvement of animals complied with ethical standards approved by legal acts of the Russian Federation, the principles of the Basel Declaration, and recommendations of the Directive 2010/63/EU for animal experiments. All animal use protocols were approved by the French National Institute of Health and Medical Research (APAFIS #16992- 2020070612319346 v2) and Local Ethics Committee of Kazan Federal University (#24/ 22.09.2020).

CONFLICT OF INTEREST

The authors declare that they have neither evident nor potential conflict of interest related to the publication of this article.

Additional information

Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2023, Vol. 59, No. 3, pp. 207–214https://doi.org/10.31857/S004445292303004X.

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Khalilov, I., Gainutdinov, A. & Khazipov, R. Bilateral Synchronization of Hippocampal Theta Oscillations in vitro. J Evol Biochem Phys 59, 719–726 (2023). https://doi.org/10.1134/S0022093023030067

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  • DOI: https://doi.org/10.1134/S0022093023030067

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