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In vitro Effects of Cold Shock on the Size and Activity of Nucleated Erythrocytes in Scorpaena porcus (Linnaeus, 1758)

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Abstract

The in vitro effect of cold shock on nucleated erythrocytes of the benthic scorpionfish Scorpaena porcus L. was studied. Blood samples were exposed to 22°C (control group) and 4°C (experimental group) for 3 h. Cold shock had no significant impact on vital characteristics of scorpionfish erythrocytes. The cells retained the integrity of cytoplasmic membranes, mitochondrial potential, and levels of oxidative processes, as evidenced by constant values of propidium iodide, rhodamine 123, and 2',7'-dichlorodihydrofluorescein diacetate fluorescence intensity. Main changes were concerned with erythrocyte shape and nucleus functional activity. While the red blood cells became more rounded, the nucleus size increased, leading to a higher nuclear–cytoplasmic ratio and a decrease in the fluorescence intensity of cells labeled with a nuclear dye SYBR Green I. Supposedly, this was due to cytoskeletal alterations and activation of transcriptional processes.

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ACKNOWLEDGMENT

The authors are grateful to E.S. Kladchenko for her methodological assistance.

Funding

This work was implemented under the governmental program 121041400077-1.

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Authors and Affiliations

  1. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Sevastopol, Russia

    N. E. Shalagina, A. A. Soldatov & Yu. V. Bogdanovich

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  1. N. E. Shalagina
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  2. A. A. Soldatov
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  3. Yu. V. Bogdanovich
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Contributions

Conceptualization and experimental design (A.A.S., N.E.Sh.), experimenting and data collection (N.E.Sh., Yu.V.B.), data processing (N.E.Sh., Yu.V.B.), writing and editing the manuscript (A.A.S., N.E.Sh.).

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Correspondence to N. E. Shalagina.

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COMPLIANCE WITH ETHICAL STANDARDS

All applicable international, national and/or institutional guidelines for the care and use of laboratory animals were observed.

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The authors declare that they have no conflict of interest.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2023, Vol. 59, No. 3, pp. 167–176https://doi.org/10.31857/S0044452923030087.

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Shalagina, N.E., Soldatov, A.A. & Bogdanovich, Y.V. In vitro Effects of Cold Shock on the Size and Activity of Nucleated Erythrocytes in Scorpaena porcus (Linnaeus, 1758). J Evol Biochem Phys 59, 676–686 (2023). https://doi.org/10.1134/S002209302303002X

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