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A Comparative Analysis of the Distribution of Different Photosensitizers in Paramecium caudatum and Amoeba proteus

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Abstract

The accumulation of different photosensitizers (PSs) (Radachlorin, Rose Bengal, Coproporphyrin) in ciliates Paramecium caudatum and amoebae Amoeba proteus were evaluated both by observations on living cells and on the fixed material, using fluorescence and confocal laser scanning microscopy. The above single-celled eukaryotic organisms shared similar intracellular distribution patterns of different PSs with mammalian cells and thus can be used as model objects at the initial stages of preclinical studies of various substances, potential PSs. Some methodological recommendations for the identification of intracellular compartments, where PSs selectively accumulate, were proposed.

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ACKNOWLEDGMENTS

The ciliates Paramecium caudatum (clone 39-9) were obtained from the “Centre for Culture Collection of Microorganisms” of the St. Petersburg State University Research Park.

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Authors and Affiliations

  1. Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia

    L. V. Chistyakova

  2. Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russia

    D. R. Faizullina & N. N. Petrishchev

  3. Alferov Federal State Budgetary Institution of Higher Education and Science, St. Petersburg National Research Academic University of the Russian Academy of Sciences, St. Petersburg, Russia

    S. V. Shmakov

Authors
  1. L. V. Chistyakova
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  2. D. R. Faizullina
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  3. S. V. Shmakov
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  4. N. N. Petrishchev
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Contributions

N.N.P.—conceptualization, research consulting; L.V.Ch.—experimental design, data collection and processing, protist culturing; D.R.F.—data collection; S.V.Sh.—CLSM studies, data processing; all co-authors—writing and editing the manuscript.

Corresponding author

Correspondence to N. N. Petrishchev.

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ETHICAL STANDARDS

All applicable international, national and/or institutional guidelines for the care and use of animals have been observed.

CONFLICT OF INTEREST

The authors declare that they have no conflicts of interest.

Additional information

Translated by A. Polyanovsky

Russian Text © The Author(s), 2022, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2022, Vol. 58, No. 6, pp. 498–506https://doi.org/10.31857/S004445292206002X.

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Chistyakova, L.V., Faizullina, D.R., Shmakov, S.V. et al. A Comparative Analysis of the Distribution of Different Photosensitizers in Paramecium caudatum and Amoeba proteus. J Evol Biochem Phys 58, 1720–1729 (2022). https://doi.org/10.1134/S0022093022060059

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