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The Effect of M-Cholinoreceptor Blockade on Functional Activity of Somatomotor, Cardiovascular and Respiratory Systems in Newborn Rats upon Activation of Cholinoreactive Structures

  • Comparative and Ontogenic Physiology
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Abstract

Activities of the somatomotor, cardiovascular and respiratory systems were investigated in conditions of impaired cholinergic regulation in rat pups aged 1 and 16 days. Acetylcholinesterase (AChE) inhibition by physostigmine (eserine), which leads to activate cholinoreactive structures, is accompanied by a unidirectional impairment of the cardiac sinus rhythm in newborn and, to a lesser extent, 16-day-old rats. Activation of cholinoreactive structures decreases the rate and regularity of breathing and also alters the pattern and parameters of motor activity. Physostigmine injection potentiates motor activity in 1-day-old and depresses it in 16-day-old rats. The blockade of muscarinic cholinoreceptors in rats of both age groups largely affects functional activity of the cardiovascular system, preventing severe cardiac arrhythmias caused by injection of the AChE inhibitor. It was shown that the formation of the definitive level of cholinergic regulation, including the maturation of the mechanisms underlying reciprocal interactions within the cholinergic system itself, is completed in the early period of rat postnatal ontogenesis. Age-related differences in responses of the somatomotor, cardiovascular and respiratory systems to changes in the level of activation of cholinoreactive structures are associated with these processes.

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References

  1. Berger-Sweeney, J. and Hohmann, C.F., Behavioral consequences of abnormal cortical development: Insights into developmental disabilities, Behav. Brain Res., 1997, vol. 86, pp. 121–142.

    Article  CAS  Google Scholar 

  2. Rüdiger, T. and Bolz, J., Acetylcholine influences growth cone motility and morphology of developing thalamic axons, Cell Adhes. Migr., 2008, vol. 2(1), pp. 30–37.

    Article  Google Scholar 

  3. Coyle, J.T. and Vamamura, H.I., Neurochemical aspects of the ontogenesis of cholinergic neurons in the rat brain, Brain Res., 1976, vol. 118, pp. 429–440.

    Article  CAS  Google Scholar 

  4. Holler, T., Berse, B., Cermak, J.M., Diebler, M.F., and Blusztajn, J.K., Differences in the developmental expression of the vesicular acetylcholine transporter and choline acetyltrasferase in the rat brain, Neurosci. Lett., 1996, vol. 212, pp. 107–110.

    Article  CAS  Google Scholar 

  5. Kuznetsov, S.V., Dmitrieva, L.E., and Sizonov, V.A., Cardiac, respiratory and motor activity in the norm and after activation of catecholaminergic systems in newborn rats, J. Evol. Biochem. Physiol., 2012, vol. 48, no. 4, pp. 430–444.

    Article  CAS  Google Scholar 

  6. Kuznetsov, S.V., Sizonov, V.A., and Dmitrieva, L.E., Change of character of intersystemic interactions in newborn rat pups under conditions of decreased central influences (urethane anesthesia), J. Evol. Biochem. Physiol., 2014, vol. 50, no. 3, pp. 234–246.

    Article  CAS  Google Scholar 

  7. Bursian, A.V., Dmitrieva, L.E., Kulaev, B.S., Polyakova, L.A., Semenova, Yu.O., Sizonov, V.A., and Timofeeva, O.P., Secondary rhythms of cardiac activity in rat ontogeny, J. Evol. Biochem. Physiol., 2003, vol. 39, no. 2, pp. 211–218.

    Article  Google Scholar 

  8. Kuznetsov, S.V. and Kuznetsova, N.N., A change in hemodynamic indices during early postnatal ontogenesis of rats after injection of a cholinesterase inhibitor eserine and in premedication by M- and N-cholinolytics, Russ. J. Physiol., 2018, vol. 104, no. 9, pp. 1075–1085.

    Google Scholar 

  9. Timofeeva, O.P., Vdovichenko, N.D., and Kuznetsov, S.V., Dynamics of the formation of rhythmic activity of the heart in fetuses and newborn rats, Bull. Exp. Biol. Med., 2011, vol. 152, no. 4, pp. 397–401.

    Article  Google Scholar 

  10. Dmitrieva, L.E. and Sizonov, V.A., Heart rhythm disturbances caused by injection of cholinesterase inhibitor physostigmine to rats during the early ontogeny, Bull. Exp. Biol. Med., 2018, vol. 165, no. 1, pp. 44–47.

    Article  Google Scholar 

  11. Losev, N.A., Sapronov, N.S., Khnychenko, L.K., and Shabanov, P.D., Farmakologiya novykh kholinergicheskikh sredstv: farmakologiya—klinike (Pharmacology of Novel Cholinergic Preparations: Pharmacology for Clinical Practice), St. Petersburg, 2015.

  12. Kuznetsov, S.V., Goncharov, N.V., and Glashkina, L.M., A change in parameters of functioning of the cardiovascular and respiratory systems in rats of different ages under effects of low doses of a cholinesterase inhibitor phosphacol, J. Evol. Biochem. Physiol., 2005, vol. 41, no. 2, pp. 201–210.

    Article  CAS  Google Scholar 

  13. Shvalev, V.N., Sosunov, A.A., and Guski, G., Morfologicheskie osnovy innervatsii serdtsa (Morphological Grounds of Heart Innervation), Moscow, 1992.

  14. Marvin, W.J. Jr., Hermsmeyer, K., McDonald, R.I., Roskoski, L.M., and Roskoski, R. Jr., Ontogenesis of cholinergic innervation in the rat heart, Circ. Res., 1980, vol. 46, pp. 690–695.

    Article  CAS  Google Scholar 

  15. Nedoma, J., Slavíková, J., and Tuček, S., Muscarinic acetylcholine receptors in the heart of rats before and after birth, Pflügers Arch., 1986, vol. 406, no. 1, pp. 45–50.

    Article  Google Scholar 

  16. Tapilina, S.V. and Abramochkin, D.V., A decrease in myocardial sensitivity to stimulation of 3 type muscarinic acetylcholine receptors in postnatal ontogenesis, Acta Naturae, 2016, vol. 8, no. 2 (29), pp. 141–146.

    Google Scholar 

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Funding

The study was implemented within the frames of the FASO Russia’s assignment “Mechanisms of formation of physiological functions in phylo- and ontogenesis as affected by endogenous and exogenous factors” (AAAA-A18-118012290373-7).

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Correspondence to V. A. Sizonov.

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Russian Text © The Author(s), 2019, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2019, Vol. 55, No. 3, pp. 179–187.

Compliance with Ethical Standards

All applicable international, national and institutional principles of handling and using experimental animals for scientific purposes were observed.

This study did not involve human subjects as research objects.

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Sizonov, V.A., Dmitrieva, L.E. & Kuznetsov, S.V. The Effect of M-Cholinoreceptor Blockade on Functional Activity of Somatomotor, Cardiovascular and Respiratory Systems in Newborn Rats upon Activation of Cholinoreactive Structures. J Evol Biochem Phys 55, 198–207 (2019). https://doi.org/10.1134/S0022093019030050

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  • DOI: https://doi.org/10.1134/S0022093019030050

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