Skip to main content
SpringerLink
Log in

The effect of hormonal stimulation on steroid levels in tissue incubates of the sterlet (Acipenser ruthenus L.)

  • Comparative and Ontogenic Biochemistry
  • Published:
Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

Sex steroid and cortisol levels were compared in Leibovitz’s L-15 cultural medium after incubation of tissue samples from the intact female and male sterlet (Acipenser rhutenus L.) and from the fishes exposed to hormonal stimulation of sexual maturation by a superactive analog of mammalian gonadotropin-releasing hormone (LH-RH-A). A higher level of 17,20β,21-trihydroxy-4-pregnen-3-one (20βS) was revealed in the cultural medium following incubation of ovarian follicles isolated from females 5 h after LH-RH-A stimulation, as compared to the data obtained for intact females. The addition of 1 μM progesterone (P4) to the cultural medium before incubation of ovarian follicles also led to an increase in the 20βS level in the incubates by the end of the experiment. Cortisol and testosterone levels in the incubates exhibited the same tendency. The blood cortisol level significantly increased in females 5 h after their hormonal stimulation. In males, showing high levels of androgens (testosterone and 11-ketotestosterone) in the blood serum before hormonal stimulation, high levels of these hormones were also detected in the cultural medium after incubation of testicular and liver tissue samples. The addition of P4 to the medium before incubation stimulated the production of sex steroids and cortisol by the gonadal fragments. Upon addition of P4 to the incubation mixture, containing liver tissue samples, the 20βS level increased.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nagahama, Y. and Yamashita, M., Regulation of oocyte maturation in fish, Dev. Growth Differ., 2008, vol. 50, pp. 195–219.

    Article  Google Scholar 

  2. Schulz, R.W., Frana, L.R., Lareyre, J.J., LeGac, F., Chiarini-Garcia, H., Nbrega, R.H., and Miura, T., Spermatogenesis in fish, Gen. Comp. Endocrinol., 2010, vol. 165, pp. 390–411.

    Article  CAS  PubMed  Google Scholar 

  3. Yaron, Z. and Levavi-Sivan, B., Endocrine regulation of fish reproduction, Encyclopedia of Fish Physiology: From Genome to Environment. Vol. 2, Farrell, A.P., Ed., Academic Press, San Diego, 2011, pp. 1500–1508.

    Chapter  Google Scholar 

  4. Amiri, B.M., Maebayashi, M., Adachi, S., Moberg, G.P., Doroshov, S.I., and Yamauchi, K., In vitro steroidogenesis by testicular fragments and ovarian follicles in a hybrid sturgeon, Bester, Fish Physiol. Biochem., 1999, vol. 21, pp. 1–14.

    Article  CAS  Google Scholar 

  5. Bukovskaya, O.S., Lambert, J.G.D., and Kime, D.E., In vitro steroidogenesis by gonads of the Russian sturgeon, Acipenser gueldenstaedti Brandt, Fish Physiol. Biochem., 1997, vol. 16, pp. 345–353.

    Article  CAS  Google Scholar 

  6. Webb, M.A.H., Feist, G.W., Trant, J.M., Van Eeneennam, J.P., Fitzpatrik, M.S., Schreck, C.B., and Doroshov, S.I., Ovarian steroidogenesis in white sturgeon (Acipenser transmontanus) during oocyte maturation and induced ovulation, Gen. Comp. Endocrinol., 2002, vol. 129, pp. 27–38.

    Article  CAS  PubMed  Google Scholar 

  7. Kime, D.E., “Classical” and “non-classical” reproductive steroids in fish, Rev. Fish Biol. Fisheries, 1993, vol. 3, pp. 160–180.

    Article  Google Scholar 

  8. Lokman, P.M., George, K.A.N., Divers, S.L., Algie, M., and Young, G., 11-ketotestosterone and IGF-1 increase the size of previtellogenic oocyte from shortfinned eel, Anguilla australis, in vitro, Reproduction, 2007, vol. 133, pp. 955–967.

    Article  CAS  PubMed  Google Scholar 

  9. Miura, T. and Miura, C., Molecular control mechanisms of fish spermatogenesis, Fish Physiol. Biochem., 2003, vol. 28, pp. 181–186.

    Article  CAS  Google Scholar 

  10. Pankhurst, N.W. and Van Der Kraak, G., Effects of stress on reproduction and growth of fish, Fish Stress and Health in Aquaculture, Iwama, G.K., Pickering, A.D., Sumpter, J.P., and Schreck, C.B., Eds., Cambridge University Press, Cambridge, 1997, pp. 73–93.

  11. Vizziano, D., Fostier, A., Loir, M., and Le Gac, F., Testis development, its hormonal regulation and spermiation induction in teleost fish, Fish Spermatology, Alavi, S.M.H., Cosson, J., Coward, K., and Rafiee, G., Eds., Alpha Science Intl., Oxford (UK), 2008, pp. 103–139.

    Google Scholar 

  12. Mayer, I., Borg, B., and Schulz, R., Conversion of 11-ketoandrostendione to 11-ketotestosterone by blood cells of six fish species, Gen. Comp. Endocr., 1990, vol. 77, pp.70–74.

    Article  CAS  PubMed  Google Scholar 

  13. Schulz, R., In vitro metabolism of steroid hormones in the liver and in blood cells of male rainbow trout (Salmo gairdneri Richardson), Gen. Comp. Endocrinol., 1986, vol. 64, pp. 312–319.

    Article  CAS  PubMed  Google Scholar 

  14. Webb, M.A.H. and Doroshov, S.I., Importance of environmental endocrinology in fisheries management and aquaculture of sturgeons, Gen. Comp. Endocrinol., 2011, vol. 170, pp. 313–321.

    Article  CAS  PubMed  Google Scholar 

  15. Bayunova, L.V., Canario, A.V.M., Semenkova, T.B., Dyubin, V.P., Sverdlova, O.A., Trenkler, I.V., and Barannikova, I.A., Sex steroids and cortisol levels in the blood of stellate sturgeon (Acipenser stellatus Pallas) during final maturation induced by LH-RH-analogue, J. Appl. Ichthyol., 2006, vol. 22, suppl. 1, pp. 334–339.

    Article  Google Scholar 

  16. Barannikova, I.A., Bayunova, L.V., Semenkova, T.B., and Gruslova, A.B., Steroid hormones in the regulation of fish migration (on example of Russian sturgeon), Ross. Fiziol. Zh. im. I.M. Sechenova, 2003, vol. 89, no. 11, pp. 1380–1387.

    CAS  PubMed  Google Scholar 

  17. Semenkova, T.B., Barannikova, I.A., Kime, D. E., McAllister, B.G., Bayunova, L.V., Dyubin, V.P., and Kolmakov, N.N., Sex steroid profiles in female and male Stellate sturgeon during final maturation induced by hormonal treatment, J. Appl. Ichthyol., 2002, vol. 18, pp. 375–381.

    Article  CAS  Google Scholar 

  18. Nazari, R.M., Modanloo, M., Ghomi, M.R., and Ovissipor, M.R., Application of synthetic hormone LHRH-A2 on the artificial propagation of Persian sturgeon Acipenser persicus, Aquacult. Int., 2010, vol. 18, pp. 837–841.

    Article  CAS  Google Scholar 

  19. Aramli, M.S., Kalbassi, M.R., and Nazari, R.M., Comparative study of sex steroid levels of Persian sturgeon, Acipenser persicus males in responding negative and positive to LHRH-A2 hormone, J. Aquac. Res. Development., 2013, vol. 4, pp. 177–179.

    Google Scholar 

  20. Semenkova, T.B., Canario, A.V.M., Bayunova, L.V., Couto, E., Kolmakov, N.N., and Barannikova, I.A., Sex steroids and oocyte maturation in the sterlet (Acipenser ruthenus L.), J. Appl. Ichthyol., 2006, vol. 22, suppl. 1, pp. 340–345.

    Article  Google Scholar 

  21. Barannikova, I.A., Bayunova, L.V., Kolmakov, N.N., and Semenkova, T.B., Dynamics of steroid hormones in blood under hormonal stimulation of maturation in Severodvinsk sterlet (Acipenser ruthenus L.), Vopr. Ikhtiol., 2005, vol. 45, no. 1, pp. 131–139.

    CAS  Google Scholar 

  22. Idler, D.R. and Truscott, B., Phylogeny of vertebrate adrenal corticosteroids, Evolution of Vertebrate Endocrine Systems, Pang, P.K.T. and Epple, A., Eds., Texas Tech University, 1980, pp. 357–372.

    Google Scholar 

  23. Bayunova, L.V., Barannikova, I.A., and Semenkova, T.B., Sturgeon stress reactions in aquaculture, J. Appl. Ichthyol., 2002, vol. 18, pp. 397–404.

    Article  CAS  Google Scholar 

  24. Barton, B.A., Stress in Fishes: A Diversity of responses with particular reference to changes in circulating corticosteroids, Integ. Comp. Biol., 2002, vol. 42, pp. 517–525.

    Article  CAS  Google Scholar 

  25. Webb, M.A.H., Allert, J.A., Kappenman, K.M., Marcos, J., Feist, G.W., Schreck, C.B., and Shackleton, C.H., Identification of plasma glucocorticoids in pallid sturgeon in response to stress, Gen. Comp. Endocrinol., 2007, vol. 154, pp. 98–104.

    Article  CAS  PubMed  Google Scholar 

  26. Chebanov, M.S., Galich, E.V., and Chmyr’, Yu.N., Rukovodstvo po razvedeniyu i vyrashchivaniyu osetrovykh ryb (Handbook on Breeding of Sturgeon Fishes), Moscow, 2004.

    Google Scholar 

  27. Artyukhin, A.N., Semenkova, T.B., Bayunova, L.V., Lunev, G.E., and Barannikova, I.A., Histological assessment of the testes coupled with determinations of sex steroid levels in Acipenser gueldenstaedtii males responding negatively to pituitary treatment, J. Appl. Ichthyol., 2006, vol. 22, suppl. 1, pp. 361–363.

    Article  Google Scholar 

  28. Alberro, A., Williot, P., and Vizziano, D., Steroid synthesis during oocyte maturation in the Siberian sturgeon Acipenser baerii, Cybium, 2008, vol. 32, pp. 255–255.

    Google Scholar 

  29. Cuisset, B., Fostier, A., Williot, P., Bennetau-Pelissero, C., and Le Menn, F., Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon Acipenser baeri B. maturing females, Fish Physiol. Biochem., 1995, vol. 14, pp. 313–322.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    L. V. Bayunova

Authors
  1. L. V. Bayunova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. V. Bayunova.

Additional information

Original Russian Text © L.V. Bayunova, 2016, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2016, Vol. 52, No. 1, pp. 17—25.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bayunova, L.V. The effect of hormonal stimulation on steroid levels in tissue incubates of the sterlet (Acipenser ruthenus L.). J Evol Biochem Phys 52, 17–27 (2016). https://doi.org/10.1134/S0022093016010026

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0022093016010026

Keywords

Search

Navigation