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Preparation and characteristics of growth and marker properties of urinary bladder mesenchymal stem cells

  • Morphological Basics for Evolution of Functions
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Abstract

Mesenchymal stem cells (MSC) are able to transdifferentiate into cells with different functional phenotypes and considered as a promising resource for regenerative therapy. MSC derived from different tissues vary in their differentiation potential and in some cases express tissue specific markers indicating a kinship between mesenchymal and parenchymal phenotypes in the same tissue. It is possible that homorganic MSC can be more effectively induced to tissue specific differentiation and preferable for cell therapy of this organ as compared with bone marrow derived cells being commonly used for this purpose. Using bladder tissue explants, we prepared primary MSC cultures from the fetal (MSC-BF) and adult syngenic BALB/c mice and characterized their abilities during long-term passaging. In contrast to the cells from adult mice, the MSC-BF cells have the ability for a sustained growth in vitro, clonogenicity and differentiation into adipose and bone cells. Similar to the bone marrow MSC, MSC-BF express the mesenchymal markers CD29, CD44, CD49f, CD90, CD105 but not the leukocyte common antigen CD45. In normal conditions, MSC-BF produce such urothelial markers as CK14 and FOXA1 although their expression level is by far lower than in the bladder tissue. The hypomethylating agent, 5-azacytidine, induces in MSC-BF the expression of the urothelial differentiation activator PPARγ and the functional urothelium markers UP1a, UP1b, UP3a, UP3b. The data obtained suggest that MSC-BF can be epigenetically reprogrammed into urothelium by the 5-azacytidine treatment, and this may offer the novel strategy for cell therapy of bladder diseases.

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Abbreviations

MSC:

mesenchymal stem cells

SC:

stem cells

MSC-BF:

fetal bladder MSC

MSC-BA:

adult bladder MSC

PCR:

polymerase chain reaction

GM:

growth medium

FBS:

fetal bovine serum

Ck:

cytokeratins

FOXA1:

forkhead box A1

PBS:

phosphate buffered saline

UP:

uroplakins

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    O. V. Zhidkova, N. S. Petrov & B. V. Popov

  2. St. Petersburg State Polytechnical University, St. Petersburg, Russia

    N. S. Petrov

Authors
  1. O. V. Zhidkova
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  2. N. S. Petrov
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  3. B. V. Popov
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Correspondence to B. V. Popov.

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Original Russian Text © O.V. Zhidkova, N.S. Petrov, B.V. Popov, 2013, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2013, Vol. 49, No. 1, pp. 67–77.

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Zhidkova, O.V., Petrov, N.S. & Popov, B.V. Preparation and characteristics of growth and marker properties of urinary bladder mesenchymal stem cells. J Evol Biochem Phys 49, 105–116 (2013). https://doi.org/10.1134/S0022093013010131

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  • DOI: https://doi.org/10.1134/S0022093013010131

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