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Effect of temperature on proteinase activites of enteral microbiota and intestinal mucosa of fish of different ecological groups

  • Comparative and Ontogenic Biochemistry
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Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

Effect of temperature on proteinases activities of enteral microbiota and of intestinal mucosa was studied in five fish species (roach Rutilus rutilus, crucian carp Carassius carassius, common perch Perca fluviatilis, pike-perch Zander lucioperca, and pike Esox lucius) belonging by the nutrition type to different ecological groups. Essential differences of temperature characteristics of proteinases of intestinal mucosa and of enteral microbiota are revealed in fish belonging to different ecological groups. The character of the t-function of proteinases of intestinal mucosa and enteral microbiota for casein and hemoglobin as a rule is different. The values of the apparent E act proteinases of intestinal mucosa for casein in most cases are higher than those of enteral microbiota, while those for hemoglobin, on the contrary, are lower. The lowest values of relative proteinase activities for casein in the zone of low temperatures (38 and 45.3% of the maximal activity) and the Eact value (less than 2.0 kcal/mol) are found at study of proteinases of enteral microbiota in common perch and crucian carp. The latter indicates a significant adaptability of the enteral microbiota proteinases of common perch and crucian carp to functioning at low temperatures.

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Authors and Affiliations

  1. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, Borok, Yaroslavl oblast, Russia

    V. V. Kuz’mina & M. V. Shalygin

  2. Yaroslavl State Agricultural Academy, Yaroslavl, Russia

    V. V. Kuz’mina, M. V. Shalygin & E. G. Skvortsova

Authors
  1. V. V. Kuz’mina
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  2. M. V. Shalygin
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  3. E. G. Skvortsova
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Correspondence to V. V. Kuz’mina.

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Original Russian Text © V.V. Kuz’mina, M.V. Shalygin, E.G. Skvortsova, 2012, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2012, Vol. 48, No. 2, pp. 120–125.

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Kuz’mina, V.V., Shalygin, M.V. & Skvortsova, E.G. Effect of temperature on proteinase activites of enteral microbiota and intestinal mucosa of fish of different ecological groups. J Evol Biochem Phys 48, 140–146 (2012). https://doi.org/10.1134/S0022093012020028

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  • DOI: https://doi.org/10.1134/S0022093012020028

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