Abstract
Fat body is the major site of peptide antibiotic synthesis in insects. The goal of this work was to search in Calliphora vicina maggot for humoral factors that induce synthesis of antimicrobial peptides by the fat body cells in trauma. The preliminary analysis has shown that the activation factor revealed in hemolymph is a thermostable hydrophilic compound with a molecular mass lower than 3 kDa. The integument epitheliocytes were also found to release the humoral factors directly stimulating synthesis of antimicrobial peptides by the fat body.
Similar content being viewed by others
References
Reichhart, J.M., Meister, M., Dimarcq, J.L., Zachary, D., Hoffmann, D., Ruiz, C., Richards, G., and Hoffmann, J.A., Insect Immunity: Developmental and Inducible Activity of the Drosophila Diptericin Promoter, EMBO J., 1992, vol. 11, pp. 1469–1477.
Price, P.W., Parasitoid Strategies and Community Organization, Environm. Entomol., 1973, vol. 2, pp. 623–626.
Chernysh, S.I., Simonenko, N.P., and Meister, M., Developmental Variability of the Antibacterial Response in Larvae and Pupae of Calliphora vicina (Diptera: Calliphoridae) and Drosophila melanogaster (Diptera: Drosophilidae), Eur. J. Entomol., 1995, vol. 92, pp. 203–209.
Hultmark, D., Immune Reactions in Drosophila and Other Insects: a Model for Innate Immunity, J. Tr. Genet., 1993, vol. 9, pp. 178–183.
Trenszec, T. and Faye, I., Synthesis of Immune Proteins in Primary Cultures of Fat Body from Hyalophora cecropia, J. Ins. Biochem., 1988, vol. 18, pp. 299–312.
Wang, L. and Ligoxygakis, P., Pathogen Recognition and Signalling in the Drosophila Innate Immune Response, Immunobiol., 2006, vol. 211, no. 4, pp. 251–261.
Brey, P.T., Lee, W.-J., Yamakawa, M., Koizumi, Y., Perrot, S., Francois, M., and Ashida, M., Role of Integument in Insect Immunity: Epicuticular Abrasion and Induction of Cecropin Synthesis in Cuticular Epithelial Cells, Proc. Natl. Acad. Sci. USA, 1993, vol. 90, pp. 6275–6279.
Royet, J., Infectious Non-Self Recognition in Invertebrates: Lessons from Drosophila and Other Insect Models, Mol. Immunol., 2004, vol. 41, no. 11, pp. 1063–1075.
Werner, T., Liu, G., Kang, D., Ekengren, S., Steiner, H., and Hultmark, D., A Family of Peptidoglycan Recognition Proteins in the Fruit Fly Drosophila melanogaster, Proc. Natl. Acad. Sci. USA, 2000, vol. 97. pp. 13 772–13 777.
Takehana, A., Yano, T., Mita, S., Kotani, A., Oshima, Y., and Kurata, S., Peptidoglycan Recognition Protein PGRP-LE and PGRP-LC Act Synergistically in Drosophila Immunity, EMBO J., 2004, vol. 23, pp. 4690–4700.
Lavine, M.D., Chen, G., and Strand, M.R., Immune Challenge Differentially Affects Transcript Abundance of Three Antimicrobial Peptides in Hemocytes from the Moth Pseudoplusia includens, Ins. Biochem. Mol. Biol., 2005, vol. 35, no. 12, pp. 1335–1346.
Brennan, C.A., Delaney, J.R., Schneider, D.S., and Anderson, K.V., Psidin Is Required in Drosophila Blood Cells for Both Phagocytic Degradation and Immune Activation of the Fat Body, Curr. Biol., 2007, vol. 17, no. 1, pp. 67–72.
Vinogradova, E.B., Blow Fly Calliphora vicina—a Model Object for Physiological and Ecological Studies, Trudy Zool. Inst. Akad. Nauk SSSR, 1984, vol. 118, Leningrad: Nauka, 272 p.
Hoffmann, A., Funkner, A., Neumann, P., Juhnke, S., Walther, M., Schierhorn, A., Weininger, U., Balbach, J., Reuter, J., and Stubbs, M.T., Biophysical Characterization of Refolded Drosophila Spätzle, a Cystine Knot Protein, Reveals Distinct Properties of Three Isoforms, J. Biol. Chem., 2008, vol. 283, no. 47, pp. 32 598–32 609.
Chernysh, S.I., Antimicrobial Substances from Insects, Proceedings of the First Korea/Russia Joint Symposium on Bioresources and Biotechnology, 1996, pp. 281–296.
Lambert, J., Keppi, E., and Dimarcq, J.-L., Insect Immunity: Isolation from Immune Blood of the Dipteran Phormia terranovae of Two Insect Antibacterial Peptides with Sequence Homology to Rabbit Lung Macrophage Bactericidal Peptides, Proc. Nat. Acad. Sci. USA, 1989, vol. 86, pp. 262–266.
Morishima, I., Yamada, K., and Ueno, T., Bacterial Peptidoglycan as Elicitor of Antibacterial Protein Synthesis in Larvae of the Silkworm, Bombyx mori, Ins. Biochem. Mol. Biol., 1992, vol. 22, no. 4, pp. 363–367.
Hoffmann, J.A. and Hoffmann, D., The Inducible Antibacterial Peptides of Dipteran Insects, 34th Forum in Immunology, 1990, pp. 910–918.
Glupov, V.V. and Bakhvalov, S.A., Insect Resistance Mechanisms in Pathogenesis, Uspekhi Sovr. Biologii, 1998, vol. 118, no. 4, pp. 466–482.
Tulin, D.V. and Chaga, O.Yu., Hemocytes of the Calliphora vicina Larva. I. Histological Analysis, Tsitologiya, 2003, vol. 45, no. 10, pp. 976–985.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A. Yu. Yakovlev, 2011, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2011, vol. 47, no. 6, pp. 461–468.
To the 100th Anniversary of A.S. Danilevsky
Rights and permissions
About this article
Cite this article
Yakovlev, A.Y. Induction of antimicrobial peptide synthesis by the fat body cells of maggots of Calliphora vicina R.-D. (Diptera, Calliphoridae). J Evol Biochem Phys 47, 543–551 (2011). https://doi.org/10.1134/S0022093011060056
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0022093011060056