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Participation of receptors of the NMDA type in regulation by glutamate of alimentary motor program of the freshwater mollusc Lymnaea stagnalis

  • Comparative and Ontogenic Physiology
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Abstract

A possible participation of receptors of the NMDA type in regulation by glutamate of the Lymnaea stagnalis alimentary program was studied in electrophysiological experiments. The specific antagonist of receptors of the N-methyl-D-aspartate (NMDA) type MK-801 has been shown to turn off the endogenous generation of the standard three-phase rhythm or the two-phase rhythm. Stimulation of receptors of this type by their specific agonist, NMDA, on the contrary, increased frequency of the alimentary rhythm and transformed it to the two-phase one. All NMDA effects are eliminated by MK-801. Apart from action on generation of central alimentary rhythms, ligands of receptors of the NMDA type change the tonical level of depolarization and activity of the alimentary circuit motoneurons. MK-801 decreased the initial level of the motoneuron B4 activity and inhibited the excitatory effect both of NMDA and of glutamate itself. There are also obtained data in favor of that earlier reported effect of transformation of the inhibitory response of neurons B4 to glutamate to the excitatory one at action of nitric oxide (NO) donors can be mediated by the specific NO effect on the activity of receptors of the NMDA type. The blocker of NMDA receptors MK-801 has been shown to inhibit the effect of transformation of the response to glutamate. The NO donor nitroprusside enhanced essentially the NMDA excitatory action, while the NO acceptor PTlO decreased it. The results obtained with use of ODQ, the blocker of NO-sensitive guanylyl cyclase (GC), allow thinking that effect of NO on activity of the NMDA receptors of the pond snail alimentary program can be realized through the metabolic pathway GC-cGMP. On the whole, the obtained results show the pond snail receptors of the NMDA type to participate in generation and rearrangements of rhythmical alimentary programs in the tonical excitatory effect on the alimentary program motoneurons in the NO-dependent transformation of the glutamate response.

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  1. Kol’tsov Institute of Development Biology, Russian Academy of Sciences, Moscow, Russia

    T. L. Dyakonova & V. E. Dyakonova

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  1. T. L. Dyakonova
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  2. V. E. Dyakonova
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Original Russian Text © T. L. Dyakonova, V. E. Dyakonova, 2010, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2010, Vol. 46, No. 1, pp. 45–51.

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Dyakonova, T.L., Dyakonova, V.E. Participation of receptors of the NMDA type in regulation by glutamate of alimentary motor program of the freshwater mollusc Lymnaea stagnalis . J Evol Biochem Phys 46, 53–60 (2010). https://doi.org/10.1134/S0022093010010068

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  • DOI: https://doi.org/10.1134/S0022093010010068

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