Skip to main content
Log in

Effect of temperature on proteinase activities in intestinal chyme and mucosa of fish of different ecological groups

  • Comparative and Ontogenic Biochemistry
  • Published:
Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

Effect of temperature on activities of proteinases in intestinal chyme and mucosa was studied in three fish species (pike-perch, zope, roach) belonging to different ecological groups by their nutrition type. There was revealed a significant difference of dependence of enzyme activities in chyme on temperature in the benthophage, roach (a higher level of relative activity in the range of lower temperatures and a wider zone of temperature optimum) as well as of values of apparent energy of activation of the protein hydrolysis process as compared with that in planktoand ichtyophages, zope and pike-perch, which indicates a significant effect of the enteral microbiota proteinases and of nutrition objects on characteristics of hydrolases functioning in fish intestine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ugolev, A.M. and Kuz’mina, V.V., Pishchevaritel’nye protsessy i adaptatsii u ryb (Digestive Processes and Adaptations in Fish), St. Petersburg, 1993.

  2. Kuz’mina, V.V., Fiziologo-biokhimicheskie osnovy ekzotrofii ryb (Physiological-Biochemical Grounds of Exotrophy in Fish), Moscow, 2005.

  3. Egorova, V.V., Iezuitova, N.N., Timofeeva, N.M., Tulyaganova, E.Kh., Gurman, E.G., Shcherbakov, G.N., and Ugolev, A.M., Some Temperature Characteristics and Temperature Adaptations of Enzymes Providing Membranous Digestion in Poikilothermal and Homoiothermal Animals, Zh. Evol. Biokhim. Fiziol., 1974, vol. 10, pp. 223–231.

    PubMed  CAS  Google Scholar 

  4. Kuz’mina, V.V., Effect of Temperature on Digestive Hydrolases of Invertebrate Animals, Zh. Evol. Biokhim. Fiziol., 1999, vol. 35, pp. 15–19.

    Google Scholar 

  5. Kuz’mina, V.V. and Pervushina, K.A., Effect of Temperature and pH on Activity of Proteinases of Intestinal Mucosa and Enteral Microbiota of Fish, Zh. Evol. Biokhim. Fiziol., 2004, vol. 40, pp. 214–219.

    PubMed  CAS  Google Scholar 

  6. Ugolev, A.M., Evolyutsiyja pishchevareniya i printsipy evolyutsii funktsii (Evolution of Digestion and Principles of Evolution of Functions), Leningrad, 1985.

  7. Lyubanskene, V., Verbitskas, Yu., Yankyavichus, K., Lyasauskene, L., Gribauskene, V., Tryapshene, O., Yuzolenene, Yu., Yastyuginene, R., Babyanskas, M., and Yankauskene, R., Obligatnyi simbioz mikroflory pishchevaritel’nogo trakta i organizma (Obligate Symbiosis of Microflora of Digestive Tract and Organism), Vilnius, 1989.

  8. Shivokene, Ya.S., Simbiontnoe pishchevarenie u gidrobiontov i nasekomykh (Symbiotic Digestion in Hydrobionts and Insects), Vilnius, 1989.

  9. Clements, K.D., Fermentation and Gastrointestinal Microorganisms in Fishes, Gastrointestinal Ecosystems and Fermentations, Mackie, R.I. and White, B.A., Eds., New York: Chapman and Hall, 1997, Ch. 6, pp. 156–198.

    Google Scholar 

  10. Anson, M., The Estimation of Pepsin, Trypsin, Papain and Cathepsin with Hemoglobin, J. Gen. Phys., 1938, vol. 22, pp. 79–83.

    Article  CAS  Google Scholar 

  11. Asgeirsson, B., Fox, J.W., and Bjarnason, J.B., Purification and Characterization of Trypsin from the Poikilotherm Gadus morhua, Eur. J. Biochem., 1989, vol. 180, pp. 85–94.

    Article  PubMed  CAS  Google Scholar 

  12. Pavlisko, A., Rial, A., and Coppes, Z., Purification and Characterization of a Protease from the Pyloric Caeca of Menhaden (Brevoortia spp) and Mullet (Mugil spp) from the Southwest Atlantic Region, J. Food Biochem., 1999, vol. 23, pp. 225–241.

    Article  CAS  Google Scholar 

  13. Bezerra, R.S., Santos, J.F., Paiva, P.G., Correia, M.T.S., Coelho, L.S.B.B., Vieira, V.L.A., and Carvalho, L.B., Jr., Partial Purification and Characterization of a Thermostable Trypsin from Pyloric Caeca of Tambaqui (Colossoma macropomum), J. Food Biochem., 2001, vol. 25, pp. 199–210.

    Article  CAS  Google Scholar 

  14. Kishimura, H., Tokuda, Y., Klomklao, S., Benjakul, S., and Ando, S., Enzymatic Characteristics of Trypsin from Pyloric Caeca of Spotted Mackerel (Scomber australasicus), J. Food Biochem., 2006, vol. 30, pp. 466–477.

    Article  CAS  Google Scholar 

  15. Pavlisko, A., Rial, A., and Coppes, Z., Characterization of Trypsin Purified from the Pyloric Caeca of the Southwest Atlantic White Croaker Micropogohias furnieri (Sciaenidae), J. Food Biochem., 1997, vol. 21, pp. 383–400.

    Article  Google Scholar 

  16. Pavlisko, A., Rial, A., Vecchi, S. de, and Coppes, Z., Properties of Pepsin and Trypsin Isolated from the Digestive Tract of Parona signata «Palometa», J. Food Biochem., 1997, vol. 21, pp. 289–308.

    Article  CAS  Google Scholar 

  17. Korostelev, S.G., Nevalennyi, A.N., and Levchenko, O.E., Characteristics of Digestive Enzymes in Intestine of the Pacific Halibut Hippoglossus stenolepis (Schmidt, 1904) and the Great Flounder Platichthys stellatus (Pallas, 1788), Biol. Morya, 2005, vol. 31, pp 221–224.

    Google Scholar 

  18. El-Shemy, M.G., Characterization of Affinity-Purified Trypsin from Hybrid Tilapia (Tilapia nilotica/aurea), J. Food Biochem., 1997, vol. 21, pp. 163–175.

    Article  CAS  Google Scholar 

  19. Zubkova, L.A., Bacterial Flora in Organs and Tissues of Carp (Cyprinus carpio L.), Trudy Kasp-NIRKh, 1965, vol. 20, pp. 117–121.

    Google Scholar 

  20. Zubkova, L.A., To the Problem on Normal Microflora in Volga Pike-Perch (Lucioperca lucioperca), Trudy KaspNIRKh, 1966, vol. 22, pp. 81–85.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Original Russian Text © V. V. Kuz’mina, E. G. Skvortsova, M. V. Shalygin, 2008, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2008, Vol. 44, No. 5, pp. 482–487.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kuz’mina, V.V., Skvortsova, E.G. & Shalygin, M.V. Effect of temperature on proteinase activities in intestinal chyme and mucosa of fish of different ecological groups. J Evol Biochem Phys 44, 569–575 (2008). https://doi.org/10.1134/S0022093008050058

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0022093008050058

Key words

Navigation