Abstract
Effect of temperature on activities of proteinases in intestinal chyme and mucosa was studied in three fish species (pike-perch, zope, roach) belonging to different ecological groups by their nutrition type. There was revealed a significant difference of dependence of enzyme activities in chyme on temperature in the benthophage, roach (a higher level of relative activity in the range of lower temperatures and a wider zone of temperature optimum) as well as of values of apparent energy of activation of the protein hydrolysis process as compared with that in planktoand ichtyophages, zope and pike-perch, which indicates a significant effect of the enteral microbiota proteinases and of nutrition objects on characteristics of hydrolases functioning in fish intestine.
Similar content being viewed by others
References
Ugolev, A.M. and Kuz’mina, V.V., Pishchevaritel’nye protsessy i adaptatsii u ryb (Digestive Processes and Adaptations in Fish), St. Petersburg, 1993.
Kuz’mina, V.V., Fiziologo-biokhimicheskie osnovy ekzotrofii ryb (Physiological-Biochemical Grounds of Exotrophy in Fish), Moscow, 2005.
Egorova, V.V., Iezuitova, N.N., Timofeeva, N.M., Tulyaganova, E.Kh., Gurman, E.G., Shcherbakov, G.N., and Ugolev, A.M., Some Temperature Characteristics and Temperature Adaptations of Enzymes Providing Membranous Digestion in Poikilothermal and Homoiothermal Animals, Zh. Evol. Biokhim. Fiziol., 1974, vol. 10, pp. 223–231.
Kuz’mina, V.V., Effect of Temperature on Digestive Hydrolases of Invertebrate Animals, Zh. Evol. Biokhim. Fiziol., 1999, vol. 35, pp. 15–19.
Kuz’mina, V.V. and Pervushina, K.A., Effect of Temperature and pH on Activity of Proteinases of Intestinal Mucosa and Enteral Microbiota of Fish, Zh. Evol. Biokhim. Fiziol., 2004, vol. 40, pp. 214–219.
Ugolev, A.M., Evolyutsiyja pishchevareniya i printsipy evolyutsii funktsii (Evolution of Digestion and Principles of Evolution of Functions), Leningrad, 1985.
Lyubanskene, V., Verbitskas, Yu., Yankyavichus, K., Lyasauskene, L., Gribauskene, V., Tryapshene, O., Yuzolenene, Yu., Yastyuginene, R., Babyanskas, M., and Yankauskene, R., Obligatnyi simbioz mikroflory pishchevaritel’nogo trakta i organizma (Obligate Symbiosis of Microflora of Digestive Tract and Organism), Vilnius, 1989.
Shivokene, Ya.S., Simbiontnoe pishchevarenie u gidrobiontov i nasekomykh (Symbiotic Digestion in Hydrobionts and Insects), Vilnius, 1989.
Clements, K.D., Fermentation and Gastrointestinal Microorganisms in Fishes, Gastrointestinal Ecosystems and Fermentations, Mackie, R.I. and White, B.A., Eds., New York: Chapman and Hall, 1997, Ch. 6, pp. 156–198.
Anson, M., The Estimation of Pepsin, Trypsin, Papain and Cathepsin with Hemoglobin, J. Gen. Phys., 1938, vol. 22, pp. 79–83.
Asgeirsson, B., Fox, J.W., and Bjarnason, J.B., Purification and Characterization of Trypsin from the Poikilotherm Gadus morhua, Eur. J. Biochem., 1989, vol. 180, pp. 85–94.
Pavlisko, A., Rial, A., and Coppes, Z., Purification and Characterization of a Protease from the Pyloric Caeca of Menhaden (Brevoortia spp) and Mullet (Mugil spp) from the Southwest Atlantic Region, J. Food Biochem., 1999, vol. 23, pp. 225–241.
Bezerra, R.S., Santos, J.F., Paiva, P.G., Correia, M.T.S., Coelho, L.S.B.B., Vieira, V.L.A., and Carvalho, L.B., Jr., Partial Purification and Characterization of a Thermostable Trypsin from Pyloric Caeca of Tambaqui (Colossoma macropomum), J. Food Biochem., 2001, vol. 25, pp. 199–210.
Kishimura, H., Tokuda, Y., Klomklao, S., Benjakul, S., and Ando, S., Enzymatic Characteristics of Trypsin from Pyloric Caeca of Spotted Mackerel (Scomber australasicus), J. Food Biochem., 2006, vol. 30, pp. 466–477.
Pavlisko, A., Rial, A., and Coppes, Z., Characterization of Trypsin Purified from the Pyloric Caeca of the Southwest Atlantic White Croaker Micropogohias furnieri (Sciaenidae), J. Food Biochem., 1997, vol. 21, pp. 383–400.
Pavlisko, A., Rial, A., Vecchi, S. de, and Coppes, Z., Properties of Pepsin and Trypsin Isolated from the Digestive Tract of Parona signata «Palometa», J. Food Biochem., 1997, vol. 21, pp. 289–308.
Korostelev, S.G., Nevalennyi, A.N., and Levchenko, O.E., Characteristics of Digestive Enzymes in Intestine of the Pacific Halibut Hippoglossus stenolepis (Schmidt, 1904) and the Great Flounder Platichthys stellatus (Pallas, 1788), Biol. Morya, 2005, vol. 31, pp 221–224.
El-Shemy, M.G., Characterization of Affinity-Purified Trypsin from Hybrid Tilapia (Tilapia nilotica/aurea), J. Food Biochem., 1997, vol. 21, pp. 163–175.
Zubkova, L.A., Bacterial Flora in Organs and Tissues of Carp (Cyprinus carpio L.), Trudy Kasp-NIRKh, 1965, vol. 20, pp. 117–121.
Zubkova, L.A., To the Problem on Normal Microflora in Volga Pike-Perch (Lucioperca lucioperca), Trudy KaspNIRKh, 1966, vol. 22, pp. 81–85.
Author information
Authors and Affiliations
Additional information
Original Russian Text © V. V. Kuz’mina, E. G. Skvortsova, M. V. Shalygin, 2008, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2008, Vol. 44, No. 5, pp. 482–487.
Rights and permissions
About this article
Cite this article
Kuz’mina, V.V., Skvortsova, E.G. & Shalygin, M.V. Effect of temperature on proteinase activities in intestinal chyme and mucosa of fish of different ecological groups. J Evol Biochem Phys 44, 569–575 (2008). https://doi.org/10.1134/S0022093008050058
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0022093008050058