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Amphetamine-produced convulsive (bursting) firings in the neuron of the giant African snail Achatina fulica: Effects of inhibitors of phosphodiesterases

  • Comparative and Ontogenic Physiology
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Abstract

Effects of inhibitors of phosphodiesterases (PDE) on bursting activity, produced by d-amphetamine (d-AM) was studied in PPa4 neurons of the giant African snail Achatina fulica F. Action of the following PDE inhibitors was analyzed: vinpocetine (selective to PDE I), erythro-9-(2-hydroxi-3-nonyl) adenine (EHNA; selective to PDE 2), milrinone (selective to PDE 3), rolipram (selective to PDE 4), sildenafil citrate (Viagra@; selective to PDE 5), and caffeine, a non-selective phosphodiesterase inhibitor. Amphetamine at a low concentration (67.5 × 10−5 M) did not produce the bursting firing in the neurons; however, the convulsive activity appeared on addition to this solution of any if the PDE inhibitors except for sildenafil. Forskolin (an adenylyl cyclase activator, 10−4 M) also decreased the neuronal threshold to the d-AM action. The bursting activity produced by d-AM did not develop after a previous administration of the protein kinase A inhibitor KT-5720. The phospholipase C blocker U73122 had no effect on the bursting activity produced by d-AM. It is concluded that the neuronal convulsive activity induced by d-AM is associated with the phosphodiesterase activity.

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Authors and Affiliations

  1. Department of Pharmacology, Medical Center, National University, Taiwan

    C. H. Lin, P. J. Lin, I. M. Chen, Y. H. Chen, P. L. Lin & M. C. Tsai

  2. Department of General Physiology, St. Petersburg State University, St. Petersburg, Russia

    V. L. Zhuravlev

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  1. C. H. Lin
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  2. P. J. Lin
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  3. I. M. Chen
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  4. Y. H. Chen
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  5. P. L. Lin
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  6. V. L. Zhuravlev
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  7. M. C. Tsai
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Additional information

Original Russian Text © C. H. Lin, P. J. Lin, I. M. Chen, Y. H. Chen, P. L. Lin, V. L. Zhuravlev, and M. C. Tsai, 2006, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2006, Vol. 42, No. 2, pp. 134–139.

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Lin, C.H., Lin, P.J., Chen, I.M. et al. Amphetamine-produced convulsive (bursting) firings in the neuron of the giant African snail Achatina fulica: Effects of inhibitors of phosphodiesterases. J Evol Biochem Phys 42, 166–173 (2006). https://doi.org/10.1134/S0022093006020086

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  • DOI: https://doi.org/10.1134/S0022093006020086

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