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Electrostatic and Structural Effects at the Adsorption of Polylysine on the Surface of the DMPS Monolayer

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The effect of the adsorption of a polypeptide on the lateral interaction of dimyristoylphosphatidylserine molecules in different phase states on the surface of a 10 mM KCl aqueous solution has been studied. Changes in the surface pressure and Volta potential induced by the adsorption of large poly-D-lysine molecules (about 200 links in a chain) have been determined at different areas per lipid molecule in a monolayer. The adsorption of macromolecules noticeably increases the elasticity of the monolayer under lateral compression in the liquid expanded state of lipid and reduces the effective dipole moment from 0.48 to 0.38 D. These properties are in qualitative agreement with X-ray reflectometry data for the lipid monolayer obtained with synchrotron radiation with a photon energy of ≈70 keV. The electron density profiles perpendicular to the surface of the aqueous subphase have been reconstructed from reflectometry data within a model approach to the structure of an interface with two and three layers. These profiles indicate the existence of a wide diffuse polymer layer (150 ± 40) Å in width at the interface of the monolayer in both the liquid expanded and liquid condensed states. A decrease in the area per molecule in the monolayer by a factor of 2 results in the doubling of the surface density of the macromolecule film. The adsorption of the polymer also affects the integral density of the layer of polar phospholipid groups, which decreases by a factor of ≈2 in the liquid expanded phase and by ∼30% in the liquid condensed phase.

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  1. H. Mohwald, in Handbook of Biological Physics, Ed. by R. Lipowsky and E. Sackmann (Elsevier Science B.V., Netherlands, Amsterdam, 1995), p. 161.

  2. C. Stefaniu, G. Brezesinski, and H. Mohwald, Adv. Colloid Interface Sci. 208, 197 (2014).

    Article  Google Scholar 

  3. G. Cevc and D. Marsh, in Cell Biology: A Series of Monographs, Ed. by E. E. Bittar (Wiley-Interscience, New York, 1987), vol. 5, p. 442.

  4. M. Loesche, E. Sackmann, and H. Mohwald, Ber. Bunsenges. Phys. Chem. 87, 848 (1983).

    Article  Google Scholar 

  5. V. M. Kaganer, H. Mohwald, and P. Dutta, Rev. Mod. Phys. 71, 779 (1999).

    Article  ADS  Google Scholar 

  6. M. C. Shih, T. M. Bohanon, J. M. Mikrut, P. Zschack, and P. Dutta, Phys. Rev. A 45, 5734 (1992).

    Article  ADS  Google Scholar 

  7. I. R. Peterson, V. Brzezinski, R. M. Kenn, and R. Steitz, Langmuir 8, 2995 (1992).

    Article  Google Scholar 

  8. C. A. Helm, P. Tippmann-Krayer, H. Mohwald, J. Als-Nielsen, and K. Kjaer, Biophys. J. 60, 1457 (1991).

    Article  Google Scholar 

  9. R. M. Kenn, K. Kjaer, and H. Mohwald, Colloids Surf. A 117, 171 (1996).

    Article  Google Scholar 

  10. K. de Meijere, G. Brezesinski, and H. Mohwald, Macromolecules 30, 2337 (1997).

    Article  ADS  Google Scholar 

  11. Y. A. Ermakov, K. Kamaraju, K. Sengupta, and S. Sukharev, Biophys. J. 98, 1018 (2010).

    Article  ADS  Google Scholar 

  12. Yu. Ermakov, K. Kamaraju, A. Dunina-Barkovskaya, K. Vishnyakova, Y. Egorov, A. Anishkin, and S. Sukharev, Biochemistry 56, 5457 (2017).

    Article  Google Scholar 

  13. N. Marukovich, M. McMurray, O. Finogenova, A. Nesterenko, O. Batishchev, and Yu. Ermakov, in Advances in Planar Lipid Bilayers and Liposomes. A Tribute to Marin D. Mitov, Ed. by A. Iglic and J. Genova (Elsevier, Academic, Amsterdam, London, San Diego, 2013), p. 139.

  14. Y. A. Ermakov, A. Z. Averbakh, A. I. Yusipovich, and S. Sukharev, Biophys. J. 80, 1851 (2001).

    Article  Google Scholar 

  15. V. E. Asadchikov, A. M. Tikhonov, Yu. O. Volkov, B. S. Roshchin, Yu. A. Ermakov, E. B. Rudakova, I. G. D’yachkova, and A. D. Nuzhdin, JETP Lett. 106, 534 (2017).

    Article  ADS  Google Scholar 

  16. A. M. Tikhonov, V. E. Asadchikov, Yu. O. Volkov, B. S. Roshchin, and Yu. A. Ermakov, J. Exp. Theor. Phys. 125, 1051 (2017).

    Article  ADS  Google Scholar 

  17. V. Honkimaki, H. Reichert, J. Okasinski, and H. Dosch, J. Synchrotr. Rad. 13, 426 (2006).

    Article  Google Scholar 

  18. E. Bitto, M. Li, A. M. Tikhonov, M. L. Schlossman, and W. Cho, Biochemistry 39, 13469 (2000).

    Article  Google Scholar 

  19. S. Malkova, F. Long, R. V. Stahelin, S. V. Pingali, D. Murray, W. H. Cho, and M. L. Schlossman, Biophys. J. 89, 1861 (2005).

    Article  Google Scholar 

  20. J. Koo, S. Park, S. Satija, A. M. Tikhonov, J. C. Sokolov, M. H. Rafailovich, and T. Koga, J. Colloid Interface Sci. 318, 103 (2008).

    Article  ADS  Google Scholar 

  21. A. M. Tikhonov, J. Phys. Chem. B 110, 2746 (2006).

    Article  Google Scholar 

  22. F. P. Buff, R. A. Lovett, and F. H. Stillinger, Phys. Rev. Lett. 15, 621 (1965).

    Article  ADS  Google Scholar 

  23. E. S. Wu and W. W. Webb, Phys. Rev. A 8, 2065 (1973).

    Article  ADS  Google Scholar 

  24. J. D. Weeks, J. Chem. Phys. 67, 3106 (1977).

    Article  ADS  Google Scholar 

  25. S. K. Sinha, E. B. Sirota, S. Garoff, and H. B. Stanley, Phys. Rev. B 38, 2297 (1988).

    Article  ADS  Google Scholar 

  26. A. Braslau, P. S. Pershan, G. Swislow, B. M. Ocko, and J. Als-Nielsen, Phys. Rev. A 38, 2457 (1988).

    Article  ADS  Google Scholar 

  27. S. V. Pingali, T. Takiue, G. Guangming, A. M. Tikhonov, N. Ikeda, M. Aratono, and M. L. Schlossman, J. Dispers. Sci. Technol. 27, 715 (2006).

    Article  Google Scholar 

  28. A. M. Tikhonov and M. L. Schlossman, J. Phys.: Condens. Matter 19, 375101 (2007).

    Google Scholar 

  29. H. Brockman, Chem. Phys. Lipids 73, 57 (1994).

    Article  Google Scholar 

  30. N. I. Marukovich, A. M. Nesterenko, and Yu. A. Ermakov, Biochemistry (Moscow) Suppl. Ser. A: Membr. Cell Biol. 9, 40 (2015).

    Article  Google Scholar 

  31. R. Yu. Molotkovskii, T. R. Galimzyanov, and Yu. A. Ermakov, Colloid. J. 81, 125 (2019).

    Article  Google Scholar 

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Correspondence to Yu. A. Ermakov or A. M. Tikhonov.

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Russian Text © The Author(s), 2019, published in Pis’ma v Zhurnal Eksperimental’noi i Teoreticheskoi Fiziki, 2019, Vol. 109, No. 5, pp. 340–346.

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Ermakov, Y.A., Asadchikov, V.E., Volkov, Y.O. et al. Electrostatic and Structural Effects at the Adsorption of Polylysine on the Surface of the DMPS Monolayer. Jetp Lett. 109, 334–339 (2019).

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