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Localized donor-acceptor pairs of fluorophores: Determination of the energy transfer rate by nonlinear fluorimetry

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Abstract

A laser fluorescence method has been developed for determining the energy transfer rate. It is based on the model of collective states of localized donor-acceptor pairs of fluorophores proposed in our previous work and involves nonlinear fluorimetry (other photophysical parameters of the donor and acceptor are simultaneously determined). The proposed approach has been tested on mRFP1 fluorescent (red) protein.

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Authors and Affiliations

  1. Faculty of Physics, Moscow State University, Moscow, 119991, Russia

    E. A. Shirshin, A. A. Banishev & V. V. Fadeev

Authors
  1. E. A. Shirshin
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  2. A. A. Banishev
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  3. V. V. Fadeev
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Correspondence to E. A. Shirshin.

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Original Russian Text © E.A. Shirshin, A.A. Banishev, V.V. Fadeev, 2009, published in Pis’ma v Zhurnal Éksperimental’noĭ i Teoreticheskoĭ Fiziki, 2009, Vol. 89, No. 10, pp. 567–570.

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Shirshin, E.A., Banishev, A.A. & Fadeev, V.V. Localized donor-acceptor pairs of fluorophores: Determination of the energy transfer rate by nonlinear fluorimetry. Jetp Lett. 89, 475–478 (2009). https://doi.org/10.1134/S0021364009100014

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  • DOI: https://doi.org/10.1134/S0021364009100014

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