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A simple laser-based device for simultaneous microbial culture and absorbance measurement

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Abstract

In this work we present a device specifically designed to study microbial growth with several applications related to environmental microbiology and other areas of research as astrobiology. The Automated Measuring and Cultivation device (AMC-d) enables semi-continuous absorbance measurements directly during cultivation. It can measure simultaneously up to 16 samples. Growth curves using low and fast growing microorganism were plotted, including Escherichia coli and Haloferax volcanii, a halophilic archaeon.

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Authors and Affiliations

  1. Instituto de Astronomía y Física del Espacio (IAFE), UBA-CONICET, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, 1428, Argentina

    X. C. Abrevaya, O. Areso & P. J. D. Mauas

  2. Laboratorio de Biosensores y Bioanálisis, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, UBA and IQUIBICEN-CONICET, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, 1428, Argentina

    E. Cortón

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  1. X. C. Abrevaya
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  2. E. Cortón
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  3. O. Areso
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  4. P. J. D. Mauas
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Correspondence to X. C. Abrevaya.

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Abrevaya, X.C., Cortón, E., Areso, O. et al. A simple laser-based device for simultaneous microbial culture and absorbance measurement. Instrum Exp Tech 56, 472–476 (2013). https://doi.org/10.1134/S0020441213030147

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  • DOI: https://doi.org/10.1134/S0020441213030147

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