Abstract
The electrochemical activity of betulin (the ability to undergo electrochemical oxidation) in aqueous solutions is determined by voltammetry on carbon-containing (glassy-carbon) electrodes. The suggested identification method is based on measuring the values of currents corresponding to the oxidation peaks of active functional groups of betulin, their heights being linearly dependent on the betulin concentration within a range of 1 × 10–6–8 × 10–5 mol/L. The developed voltammetric procedure makes it possible to determine betulin in plant extracts. Voltammetric curves for dry betulin extracts prepared using different purification procedures are obtained to demonstrate the possibility of betulin determination. The results are confirmed in spiking tests.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Tolstikov, G.A., Flekhter, O.B., Shultz, E.E., et al., Betulin and its derivatives. Chemistry and Biological activity, Chem. Sustainable Dev., 2005, no. 3, pp. 1–29.
Mikhailenko, M.A., Shakhtshneider, T.P., Brezgunova, M.E., et al., Preparation and research of physical and chemical properties of betulin solvates, Khim. Rastit. Syr’ya, 2010, no. 2, pp. 63–70.
Levdanskii, V.A., Polezhaeva, N.I., Levdanskii, A.V., and Kuznetsov, B.N., Study of birch bark extraction with hexane, ethyl acetate, isopropyl alcohol and water, Khim. Rastit. Syr’ya, 2004, no. 2, pp. 17–20.
Ashavina, O.Yu., Kabal’nova, N.N., Flekhter, O.B., et al., Oxidation of betulin and its acetates with dimethyldioxirane, Mendeleev Comm., 2004, vol. 14, no. 5, pp. 221–223.
Kuznetsova, S.A., Vasil’eva, N.Yu., Kalacheva, G.S., et al., Preparation of betulin diacetate from birch bark birch bark and study of its antioxidant activity, Zh. Sib. Fed. Univ., Khim., 2008, vol. 1, no. 2, pp. 151–165.
Koptelova, E.N., Kutakova, N.A., and Tret’yakov, S.I., Determination of composition of the ethanol extract from birch bark, Izv. Vyssh. Uchebn. Zaved., Lesn. Zh., 2011, no. 6, pp. 107–111.
Zhao, G., Simultaneous determination of betulin and betulinic acid in white birch bark using PR-HPLC, J. Pharm. Biomed. Anal., 2007, vol. 43, no. 3, pp. 959–962.
Li, W., Li, X., Li, Z.-L., et al., Chemical components of carpophorus Xanthoceras sorbifolia Bunge, J. Shenyang Pharm. Univ., 2005, vol. 22, no. 5, pp. 345–347.
Zuman, P., Past, present, and future of applications of electroanalytical techniques in analytical and physical organic chemistry, J. Solid State Electrochem., 2011, vol. 15, pp. 1753–1759.
Tyszczuk-Rotko, K., Wojciak-Kosior, M., and Sowa, I., Voltammetric determination of betulinic acid at lead film electrode after chromatographic separation in plant material, Anal. Biochem., 2013, vol. 436, no. 2, pp. 121–126.
Kuznetsova, S.A., Skvortsova, G.P., Malyar, Yu.N., et al., Isolation of betulin from birch bark and study of it’s physicochemical and pharmacological properties, Khim. Rastit. Syr’ya, 2013, no. 2, pp. 93–100.
Flekhter, O.B., Ashavina, O.Yu., Smirnova, I.E., et al., Selective oxidation of triterpene alcohols by sodium hypochlorite, Chem. Nat. Compd., 2004, vol. 40, no. 2, pp. 141–143.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated by Yu. Modestova
Rights and permissions
About this article
Cite this article
Kaliyeva, S.S., Slepchenko, G.B., Akeneev, Y.A. et al. Voltammetric Determination of Betulin in the Extracts of Plant Origin. Inorg Mater 54, 1407–1411 (2018). https://doi.org/10.1134/S0020168518140108
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0020168518140108