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Effect of a peptide modeling the nicotinic receptor binding site on the spectral and luminescent properties of dye complexes with cucurbit[8]uril

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Abstract

The paper presents the results of analysis of the effect of a high-affinity peptide (HAP) homologous to a fragment of the nicotinic acetylcholine receptor (nAChR) on the absorption and fluorescence spectra of thiazole orange and thioflavin T complexes with cucurbit[8]uril in aqueous solution. In the presence of HAP, a change in the absorption spectra of the dye complexes and a drop in the fluorescence intensity occur; for thiazole orange, the fluorescence intensity is restored to the initial level in the presence of α-bungarotoxin capable of high-affinity binding to nAChR. The proposed method make it possible to detect the presence of α-bungarotoxin.

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References

  1. Harel, M., Kasher, R., Nicolas, A., Guss, J.M., Balass, M., Fridkin, M., Smit, A.B., Brejc, K., Sixma, T.K., Katchalski-Katzir, E., Sussman, J.L., and Fuchs, S., Neuron, 2001, vol. 32, no. 2, p. 265.

    Article  CAS  Google Scholar 

  2. Tsetlin, V., Utkin, Y., and Kasheverov, I., Biochem. Pharmacol., 2009, no. 78, p. 720.

    Article  CAS  Google Scholar 

  3. Armitage, B.A., Top. Heterocycl. Chem., 2008, no. 14, p. 11.

    Article  CAS  Google Scholar 

  4. Shank, N.I., Zanotti, K.J., Lanni, F., Berget, P.B., and Armitage, B.A., J. Am. Chem. Soc., 2009, vol. 131, no. 36, p. 12960.

    Article  CAS  Google Scholar 

  5. Murudkar, S., Mora, A.K., Singh, P.K., and Nath, S., Chem. Commun., 2012, vol. 48, no. 43, p. 5301.

    Article  CAS  Google Scholar 

  6. Park, J.W., Kim, Y., Lee, K.-J., and Kim, D.J., Bioconjugate Chem., 2012, vol. 23, no. 2, p. 350.

    Article  CAS  Google Scholar 

  7. Okamoto, A., Chem. Soc. Rev., 2011, vol. 40, no. 12, p. 5815.

    Article  CAS  Google Scholar 

  8. Netzel, T.L., Nafisi, K., Zhao, M., Lenhard, J.R., and Johnson, I., J. Phys. Chem., 1995, vol. 99, no. 51, p. 17936.

    Article  CAS  Google Scholar 

  9. Karunakaran, V., Lustres, Pe J.L.F., Zhao, L., Ernsting, N.P., and Seitz, O., J. Am. Chem. Soc., 2006, vol. 128, no. 9, p. 2954.

    Article  CAS  Google Scholar 

  10. Furstenberg, A., Julliard, M.D., Deligeorgiev, T.G., Gadjev, N.I., Vasilev, A.A., and Vauthey, E., J. Am. Chem. Soc., 2006, vol. 128, no. 23, p. 7661.

    Article  Google Scholar 

  11. Nygren, J., Svanvik, N., and Kubista, M., Biopolymers, 1998, vol. 46, no. 1, p. 39.

    Article  CAS  Google Scholar 

  12. Berezin, M.Y., Guo, K., Akers, W., Livingston, J., Solomon, M., Lee, H., Liang, K., Agee, A., and Achilefu, S., Biochemistry, 2011, vol. 50, no. 13, p. 2691.

    Article  CAS  Google Scholar 

  13. Eriksson, M., Hardelin, M., Larsson, A., Bergenholtz, J., and Akerman, B., J. Phys. Chem. B, 2007, vol. 111, no. 5, p. 1139.

    Article  CAS  Google Scholar 

  14. Tatikolov, A.S., J. Photochem. Photobiol. C: Photochem. Rev., 2012, no. 13, p. 55.

    Article  CAS  Google Scholar 

  15. Biver, T., Boggioni, A., Secco, F., Turriani, E., Venturini, M., and Yarmoluk, S., Arch. Biochem. Biophys., 2007, vol. 465, no. 1, p. 90.

    Article  CAS  Google Scholar 

  16. Lubitz, I., Zikich, D., and Kotlyar, A., Biochemistry, 2010, vol. 49, no. 17, p. 3567.

    Article  CAS  Google Scholar 

  17. Biancardi, A., Biver, T., Marini, A., Mennucci, B., and Secco, F., Phys. Chem. Chem. Phys., 2011, vol. 13, no. 27, p. 12595.

    Article  CAS  Google Scholar 

  18. Choudhury, S.D., Bhasikuttan, A.S., Pal, H.L, and Mohanty. J., Langmuir, 2011, vol. 27, no. 20, p. 12312.

    Article  Google Scholar 

  19. Cao, J., Wu, T., Hu, C., Liu, T., Sun, W., Fan, J., and Peng, X., Phys. Chem. Chem. Phys., 2012, vol. 14, no. 39, p. 13702.

    Article  CAS  Google Scholar 

  20. Lau, V. and Heyne, B., Chem. Commun., 2010, vol. 46, no. 20, p. 3595.

    Article  CAS  Google Scholar 

  21. Mohanty, J., Thakur, N., Choudhury, S.D., Barooah, N., Pal, H., and Bhasikuttan, A.C., J. Phys. Chem. B, 2012, vol. 116, no. 1, p. 130.

    Article  CAS  Google Scholar 

  22. Sulatskaya, A.I. and Kuznetsova, I.M., Tsitologiya, 2010, vol. 52, no. 11, p. 955.

    CAS  Google Scholar 

  23. Antokhin, A.M., Andreev, O.I., Gainullina, E.T., Kaurov, N.E., Ryzhikov, S.B., and Taranchenko, V.F., RU Patent No. 2 386 120 (2010).

    Google Scholar 

  24. Bhasikuttan, A.C., Choudhury, S.D., Pal, H., and Mohanty, J., Isr. J. Chem., 2011, vol. 51, no. 5/6, p. 634.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Photochemistry Center, Russian Academy of Sciences, ul. Novatorov 7a, bldg. 1, Moscow, 119421, Russia

    G. V. Zakharova, R. R. Konstantinov, A. V. Odinokov, A. K. Chibisov & M. V. Alfimov

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    I. E. Kasheverov, Yu. N. Utkin, M. N. Zhmak & V. I. Tsetlin

Authors
  1. G. V. Zakharova
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  2. R. R. Konstantinov
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  3. A. V. Odinokov
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  4. A. K. Chibisov
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  5. M. V. Alfimov
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  6. I. E. Kasheverov
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  7. Yu. N. Utkin
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  8. M. N. Zhmak
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  9. V. I. Tsetlin
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Corresponding author

Correspondence to G. V. Zakharova.

Additional information

Original Russian Text © G.V. Zakharova, R.R. Konstantinov, A.V. Odinokov, A.K. Chibisov, M.V. Alfimov, I.E. Kasheverov, Yu.N. Utkin, M.N. Zhmak, V.I. Tsetlin, 2016, published in Khimiya Vysokikh Energii, 2016, Vol. 50, No. 2, pp. 127–131.

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Zakharova, G.V., Konstantinov, R.R., Odinokov, A.V. et al. Effect of a peptide modeling the nicotinic receptor binding site on the spectral and luminescent properties of dye complexes with cucurbit[8]uril. High Energy Chem 50, 121–126 (2016). https://doi.org/10.1134/S0018143916020090

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  • DOI: https://doi.org/10.1134/S0018143916020090

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