Abstract
The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles (“superquenching”), characterized by a high Stern-Volmer constant of K SV ≥ 3.3 × 107 L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).
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Original Russian Text © E.S. Lisitsyna, O.N. Lygo, N.A. Durandin, O.V. Dement’eva, V.M. Rudoi, V.A. Kuzmin, 2012, published in Khimiya Vysokikh Energii, 2012, Vol. 46, No. 6, pp. 458–463.
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Lisitsyna, E.S., Lygo, O.N., Durandin, N.A. et al. Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles. High Energy Chem 46, 363–367 (2012). https://doi.org/10.1134/S0018143912060057
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DOI: https://doi.org/10.1134/S0018143912060057