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Spectral and kinetic investigation of interaction of 3,3′-diethyl-9-chlorothiacarbocyanine perchlorate with DNA

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Abstract

Interaction of the meso-substituted carbocyanine dye 3,3′-diethyl-9-chlorothiacarbocyanine (DCTC) perchlorate with DNA in phosphate buffer solutions (pH 7) was studied by spectral and kinetic methods. It was shown that the dye mainly exists in the cis-isomer form in water and aqueous-alcoholic solutions. The interaction with DNA leads to the formation of a trans-DCTC complex, which gradually (over a few tens of minutes) converts into the complex of the dye cis-isomer. It was revealed that the reactant concentration and ionic strength of solution have a considerable effect on the kinetic characteristics of trans-cis-reorganization of DCTC complexes, thus indicating a complex character of the process.

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Authors and Affiliations

  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia

    P. G. Pronkin, A. S. Tatikolov & V. A. Kuzmin

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  1. P. G. Pronkin
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  2. A. S. Tatikolov
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  3. V. A. Kuzmin
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Original Russian Text © P.G. Pronkin, A.S. Tatikolov, V.A. Kuzmin, 2007, published in Khimiya Vysokikh Energii, 2007, Vol. 41, No. 2, pp. 129–134.

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Pronkin, P.G., Tatikolov, A.S. & Kuzmin, V.A. Spectral and kinetic investigation of interaction of 3,3′-diethyl-9-chlorothiacarbocyanine perchlorate with DNA. High Energy Chem 41, 97–102 (2007). https://doi.org/10.1134/S0018143907020087

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  • DOI: https://doi.org/10.1134/S0018143907020087

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