Abstract
The effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in developing countries according to the World Health Organization. Various technologies have recently been proposed to restore the damaged surface of the cornea. Use of biodegradable silk-based materials, including recombinant analogs of the spider silk protein spidroin, is an important avenue of research in the field of wound healing and corneal regeneration. Spidroins are well known for their optimal balance of strength and elasticity. Given their biological compatibility, lack of immunogenicity, and biodegradability, spidroins provide a biomaterial for tissue engineering and regenerative medicine. HGs based on RS rS2/12-RGDS were therefore tested for cytotoxicity toward isolated corneal epithelial cells and keratocytes with regard to possible changes in cell phenotype and migratory activity. A promising outlook and therapeutic potential were demonstrated for RS-based HGs.
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Producer strain culturing, RS isolation and purification, and HG preparation and sterilization were carried out within the state assignment of NRC “Kurchatov Institute.”
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All studies were conducted in accordance with the principles of biomedical ethics as outlined in the 1964 Declaration of Helsinki and Its later amendments. Donor material (cadaver cornea and their derivative isolated stromal cells) were used as biomaterial for experimental research. The studies were carried out in vitro in accordance with authorized procedures and a special legal approval from the Novosibirsk Oblast Division of the Federal Service for Surveillance in Healthcare (license no. L041-00110-47/00574034 dated April 7, 2020, which allows the use of tissues isolated from cadaver human eyes for transplantation and scientific research purposes).
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The authors of this work declare that they have no conflicts of interest.
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Translated by T. Tkacheva
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Agapova, O.I., Ostrovsky, D.S., Khubetsova, M.K. et al. Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells. Dokl Biol Sci 513 (Suppl 1), S41–S44 (2023). https://doi.org/10.1134/S0012496623600173
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DOI: https://doi.org/10.1134/S0012496623600173