Abstract
Proteins with clusters of C2H2 zinc finger domains (C2H2-proteins) constitute the most abundant class of transcription factors in higher eukaryotes. N-terminal ZAD (zinc finger-associated domain) dimerization domain has been identified in a large group of C2H2-proteins mostly in insects. The piragua gene encodes one of these proteins, Fu2. We have generated CRISPR/Cas9-mediated deletion of the piragua gene that has no phenotype. We have used φC31-mediated attP/attB recombination to generate a transgenic line expressing Fu2 protein fused with HA epitope. This line will be useful for analysis of DNA binding profile and functions of Fu2 protein.
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Funding
This work was supported by the Russian Science Foundation, project no. 19-74-30026.
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Translated by M. Bibov
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Sokolov, V.V., Georgiev, P.G. & Kyrchanova, O.V. The Piragua Gene Is not Essential for Drosophila Development. Dokl Biol Sci 501, 197–200 (2021). https://doi.org/10.1134/S0012496621060089
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DOI: https://doi.org/10.1134/S0012496621060089