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Increased Expression of the Multimerin-1 Gene in α-Synuclein Knokout Mice

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Abstract

Multimerin-1 (Mmrn-1) is a soluble protein, also known as elastin microfibril interfacer 4 (EMILIN-4), found in platelets and in the endothelium of blood vessels. Its function and role in pathology are still not fully understood. Genetic modifications in alpha-synuclein gene (Snca) locus that mapped 160 Kb apart from Mmrn-1 in mouse genome, could weigh with regulatory elements of Mmrn-1 gene. We have studied the Mmrn-1 expression in brain cortex of three mouse lines with Snca knock-out: B6(Cg)-Sncatm1.2Vlb/J, B6;129-Sncatm1Sud/J, and B6;129X1-Sncatm1Rosl/J. The 35-fold increase for Mmrn-1 mRNA level have been found in B6;129X1-Sncatm1Rosl/J mice that carry in their genome foreign sequences including bacterial gene neo and a strong promoter of a mouse phosphoglycerate kinase (Pgk1) oriented towards Mmrn-1 gene. This effect on regulatory elements of Mmrn-1 gene as a result of modifications in Snca locus should be taken into consideration when using B6;129X1-Sncatm1Rosl/J line, that is widely applied for study of neurodegeneration mechanisms.

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Funding

This study was supported by the Russian Science Foundation (project no. 19-14-00064). Bioinformatic analysis was supported by the Russian Foundation for Basic Research (project no. 18-04-00515). The animal facilities and equipment of the Centre for “Collective Use of IPAC RAS” were used.

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Authors and Affiliations

  1. Institute of Physiologically Active Compounds, Russian Academy of Sciences, 142432, Chernogolovka, Moscow oblast, Russia

    K. D. Chaprov, T. V. Tarasova, E. V. Teterina & R. K. Ovchinnikov

  2. School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom

    K. D. Chaprov, V. V. Goloborshcheva & T. V. Tarasova

  3. Institute of General Pathology and Pathophysiology, 125315, Moscow, Russia

    V. V. Goloborshcheva, V. G. Kucheryanu &  S. G. Morozov

  4. Research Institute of Living Systems Pharmacology, Belgorod National Research University, 308007, Belgorod, Russia

    M. V. Korokin, V. O. Soldatov & M. V. Pokrovskiy

Authors
  1. K. D. Chaprov
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  2. V. V. Goloborshcheva
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  3. T. V. Tarasova
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  4. E. V. Teterina
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  5. M. V. Korokin
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  6. V. O. Soldatov
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  7. M. V. Pokrovskiy
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  8. V. G. Kucheryanu
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  9. S. G. Morozov
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  10. R. K. Ovchinnikov
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Correspondence to K. D. Chaprov.

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Conflict of interest. The authors declare no conflict of interest.

Statement of the welfare of animals. Experiments with animals were performed according to the order no. 199n of the Ministry of Healthcare of the Russian federation from April 1, 2016 “On approval of the rules of good laboratory practice.”

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Translated by M. Stepanichev

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Chaprov, K.D., Goloborshcheva, V.V., Tarasova, T.V. et al. Increased Expression of the Multimerin-1 Gene in α-Synuclein Knokout Mice . Dokl Biol Sci 494, 260–263 (2020). https://doi.org/10.1134/S0012496620050014

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  • DOI: https://doi.org/10.1134/S0012496620050014

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