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A defensin fragment regulates CHO-K1 cell spreading and induces renewal of fatty acid composition in cell membrane phospholipids

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Abstract

A tetrapeptide defensin fragment has been shown to stimulate the spreading of CHOK1 cells. The tetrapeptide investigated had virtually no effect on the composition of cell membrane phospholipids but participated in the regulation of the renewal of fatty acid composition of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol. Incubation of cells with the peptide resulted in a change in the composition of the unsaturated fatty acid residues in the phospholipids investigated: specifically, the content of monoenoic and/or dienoic acids increased and that of polyenoic acids decreased. The possible role of the peptide investigated (1) in the regulation of the functional activity of integrin receptors, and (2) in changes in the packing density of the phospholipid acyl chains in cell membrane microdomains, which affects the rates of integrin clustering and adhesion complex formation, is discussed.

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Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    V. P. Ivanova & A. I. Krivchenko

  2. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    Z. V. Kovaleva

Authors
  1. V. P. Ivanova
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  2. Z. V. Kovaleva
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  3. A. I. Krivchenko
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Correspondence to V. P. Ivanova.

Additional information

Original Russian Text © V.P. Ivanova, Z.V. Kovaleva, A.I. Krivchenko, 2015, published in Doklady Akademii Nauk, 2015, Vol. 462, No. 6, pp. 719–722.

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Ivanova, V.P., Kovaleva, Z.V. & Krivchenko, A.I. A defensin fragment regulates CHO-K1 cell spreading and induces renewal of fatty acid composition in cell membrane phospholipids. Dokl Biol Sci 462, 167–170 (2015). https://doi.org/10.1134/S0012496615030126

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  • DOI: https://doi.org/10.1134/S0012496615030126

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