Abstract
We have previously shown that short-term moderate (30°C) hypothermia contributed to a significant increase in the intensity of free radical processes and changes in a number of bioenergetic parameters of mitochondria, while its prolongation for 3 h led to their normalization, which might be associated with the modulation of the activity and catalytic properties of succinate dehydrogenase. This study showed that moderate hypothermia, especially prolonged for 3 h, significantly increased the rate of succinate dehydrogenase catalysis and affected its dependence on concentration and temperature. The modulation of succinate dehydrogenase activity during hypothermia occurred due to changes in its kinetic and thermodynamic characteristics, of which the most significant contribution to the efficiency of enzyme catalysis was made by the parameter Vmax, maximum speed. These changes might play an important role in preserving the energy-producing function of mitochondria and reducing the intensity of free radical processes in them.
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The work was carried out within the framework of the State Task, project no. FZNZ-2020-0002.
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Statement of the welfare of humans or animals. During the study, all the norms and rules for performing experimental work using laboratory animals were observed (Directive 2010/63/EU of the Council of the European Community on the Protection of Animals Used for Experimental and Other Scientific Purposes).
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Translated by E. Puchkov
Abbreviations: ETC, electron transport chain; SDH, succinate dehydrogenase; FAD, flavin adenine dinucleotide; ROS, reactive oxygen species.
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Khalilov, R.A., Dzhafarova, A.M. & Abdullaev, V.R. Activity and Catalytic Characteristics of Rat Liver Mitochondrial Succinate Dehydrogenase under Moderate Hypothermia. BIOPHYSICS 67, 948–959 (2022). https://doi.org/10.1134/S0006350922060094
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DOI: https://doi.org/10.1134/S0006350922060094