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Assessment of Blood Clot Formation in the Rabbit Artery upon Implantation of Polyurethane Vascular Prosthesis Treated with Nitrogen Plasma

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Abstract

Vascular prosthesis samples were synthesized from polyurethane based on polypropylene glycol terminated with 2,4-toluylenediisocyanate. The outer and inner surfaces of the implants were treated with high-energy nitrogen ions of 20 and 10 keV, respectively. As a result of ion-plasma treatment, an active hydrophilic carbon nanolayer was formed on the implant surfaces. The tropoelastin protein was applied to the inner and outer surfaces of the samples treated by the ion-plasma method. Plasma-treated and untreated prosthetic specimens were implanted into the carotid artery of rabbits. After 1 week of exposure, a morphological assessment of the tissues surrounding the walls of intravascular prostheses was carried out. It was shown that blood clots had formed inside all the prostheses. However, the morphological picture of the tissues around the walls of plasma-treated implants was characterized by accelerated channeling of clots and endothelial proliferation from the intima of the vessel compared to untreated implants.

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ACKNOWLEDGMENTS

We are grateful to the researchers A.V. Kondyurin and I.V. Kondyurina from Evingar Scientific and Prof. M. Bilek, Prof. D. Mackenzie, and Prof. A. Weiss of the University of Sydney for permission to work in their laboratories and provision of the protein tropoelastin.

Funding

The work was carried out with the support of the Russian Foundation for Basic Research and Perm krai, project no. 20-48-596014, as well as the State Assignment of the IMSS of the Ural Branch of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Institute of Continuous Media Mechanics, Ural Branch, Russian Academy of Sciences, 614013, Perm, Russia

    V. S. Chudinov & I. N. Shardakov

  2. Perm State Medical University, 614990, Perm, Russia

    V. V. Litvinov & G. G. Friend

  3. Perm National Research Polytechnic University, 614990, Perm, Russia

    S. Yu. Solodnikov

  4. St. Petersburg Research Institute of Vaccines and Serums, Federal Medical-Biological Agency of Russia, 198320, St. Petersburg, Krasnoe Selo, Russia

    V. V. Maslova

Authors
  1. V. S. Chudinov
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  2. I. N. Shardakov
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  3. V. V. Litvinov
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  4. G. G. Friend
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  5. S. Yu. Solodnikov
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  6. V. V. Maslova
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Corresponding author

Correspondence to V. S. Chudinov.

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Statement of the welfare of animals. Surgical interventions, maintenance, nutrition, and euthanasia of animals met the requirements of the European Convention for the Protection of Vertebrates Used for Experiments or Other Scientific Purposes.

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Translated by E. Puchkov

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Chudinov, V.S., Shardakov, I.N., Litvinov, V.V. et al. Assessment of Blood Clot Formation in the Rabbit Artery upon Implantation of Polyurethane Vascular Prosthesis Treated with Nitrogen Plasma. BIOPHYSICS 67, 1011–1016 (2022). https://doi.org/10.1134/S0006350922060069

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