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Functional Magnetic Resonance Spectroscopy Study of Total Glutamate and Glutamine in the Human Visual Cortex Activated by a Short Stimulus

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Abstract—Glutamate+glutamine (Glx) dynamics with 2s time resolution in the human occipital cortex in response to short visual stimulation was studied using functional magnetic resonance spectroscopy (fMRS) at 3 T. The dynamic changes in the Glx level and the BOLD signal were acquired in response to a 3 s flickering checkerboard in the occipital cortex of 25 volunteers. Glx concentration increased at 1 s and 15 s after the stimulus presentation. No correlation between the amplitude of the BOLD response and the change in Glx was found. The time course of changes in the total glutamate+glutamine level after the stimulus does not correspond to the turnover rate of the glutamine-glutamate cycle, but is consistent with the temporal characteristics of the vesicular cycle: the release of glutamate from vesicles and its reuptake. Thus, it can be assumed that the observed dynamics of glutamate and glutamine is not due to metabolic transformations in the neurotransmitter cycle, but due to a change in the mobility of glutamate upon exit from the vesicles and its re-entering back.

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Funding

The work was carried out with financial support of Russian Foundation for Basic Research (Grant/Award no. 19-29-10040) and Russian Scientific Foundation (Grant/Award no. 18-1300030).

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Authors and Affiliations

  1. Clinical and Research Institute of Emergency Pediatric Surgery and Traumatology, 119180, Moscow, Russia

    A. Yakovlev, A. Manzhurtsev, M. Ublinskiy, I. Melnikov, T. Akhadov & N. Semenova

  2. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

    A. Yakovlev, P. Menshchikov & N. Semenova

  3. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334, Moscow, Russia

    A. Yakovlev, A. Manzhurtsev, M. Ublinskiy & N. Semenova

  4. Moscow State University, 119991, Moscow, Russia

    A. Manzhurtsev, T. Akhadov & N. Semenova

  5. LLC Philips Healthcare, 123022, Moscow, Russia

    P. Menshchikov & D. Kupriyanov

Authors
  1. A. Yakovlev
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  2. A. Manzhurtsev
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  3. P. Menshchikov
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  4. M. Ublinskiy
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  5. I. Melnikov
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  6. D. Kupriyanov
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  7. T. Akhadov
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  8. N. Semenova
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Corresponding author

Correspondence to A. Yakovlev.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. The study was approved by the Clinical and Research Institute of Emergency Pediatric Surgery and Trauma Ethics Committee. All participants were informed about the details of the study and provided written informed consent. All procedures were in accordance with the 1964 Helsinki Declaration and its later amendments.

Additional information

Abbreviations: fMRS, functional 1H magnetic resonance spectroscopy; Glu, glutamate; Gln, glutamine; Glx, sum of glutamate and glutamine; BOLD response, blood oxygenation level dependent response; fMRI, functional magnetic resonance imaging; NAA, N-acetylaspartate; tNAA, the sum of N-acetylaspartate and N-acetylaspartylglutamate; Cr, the sum of creatine and osphocreatine; tCho, choline-containing compounds.

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Yakovlev, A., Manzhurtsev, A., Menshchikov, P. et al. Functional Magnetic Resonance Spectroscopy Study of Total Glutamate and Glutamine in the Human Visual Cortex Activated by a Short Stimulus. BIOPHYSICS 67, 265–273 (2022). https://doi.org/10.1134/S0006350922020245

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  • DOI: https://doi.org/10.1134/S0006350922020245

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