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The Effect of an Alternating Magnetic Field on the Chemiluminescence of Human Peripheral Blood Mononuclear Cells and the Production of Pro-Inflammatory Cytokines

Abstract—It has been shown that in vitro exposure to an alternating magnetic field with a strength of 550 ± 30 A/m at a frequency of 8.6 and 9.0 Hz leads to a significant change in the chemiluminescence of the suspension of mononuclear cells isolated from the peripheral blood of healthy donors and patients with epidermolysis bullosa (EB). The enzyme-linked immunosorbent assay determined the content of pro-inflammatory cytokines (interleukin-1; interleukin-6 and tumor necrosis factor) in the peripheral blood serum of healthy donors and patients with bullous epidermolysis after in vitro exposure of blood samples to alternating magnetic field in the frequency range from 7.0 to 9.0 Hz. It was found that in the blood serum of patients with epidermolysis bullosa the content of interleukin-6 was significantly increased in comparison with healthy donors. The action of a magnetic field with frequencies from 7.0 to 7.8 Hz and from 8.6 to 9.0 Hz on blood samples from patients with epidermolysis bullosa reduced the concentration of this cytokine to the values of the reference interval. The initial indices of tumor necrosis factor in native samples of both groups remained within the normal range: 5.2 pg/mL in the control and 4.3 pg/mL in epidermolysis bullosa. The impact of the magnetic field did not lead to the parameters going beyond the threshold values, while there was a similarity in the response in the control and experimental groups. Thus, the treatment with magnetic field with frequencies of 7.4 and 7.8 Hz increased the concentration of tumor necrosis factor in both groups and a magnetic field with frequencies higher than 7.8 Hz led to a sharp decrease in it.

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Funding

The study was performed out with the financial support of the Kuban Science Foundation within the framework of the scientific project no. 20.1/119.

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Correspondence to E. E. Tekutskaya.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Translated by V. Mittova

Abbreviations: MNC, mononuclear cells; IL, interleukin; α-TNF, tumor necrosis factor alpha; EB, epidermolysis bullosa; MF, magnetic field; ROS, reactive oxygen species; CL, chemiluminescence.

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Tekutskaya, E.E., Gusaruk, L.R. & Ilchenko, G.P. The Effect of an Alternating Magnetic Field on the Chemiluminescence of Human Peripheral Blood Mononuclear Cells and the Production of Pro-Inflammatory Cytokines. BIOPHYSICS 67, 92–99 (2022). https://doi.org/10.1134/S0006350922010183

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  • DOI: https://doi.org/10.1134/S0006350922010183

  • Keywords: peripheral blood mononuclear cells
  • chemiluminescence
  • pro-inflammatory cytokines
  • alternating magnetic field
  • reactive oxygen species