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Levels of Circulating DNA in Blood Serum and DNA Damage in Leukocytes of Healthy Donors of Different Genders and Ages

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Abstract

We studied the levels of extracellular nuclear and mitochondrial DNA of blood serum and DNA damage in leukocytes of healthy donors of different sex and age groups. The baseline levels of DNA damage in leukocytes and serum DNA levels were shown to vary greatly among different donors. The baseline level of DNA damage in leukocytes was not associated with the presence of chronic deceases or an occupational health risk for elderly donors. It was found that extracellular DNA concentrations were generally higher in men than in women. There is a tendency towards an increase in the relative mitochondrial DNA copy number determined by ΔCt in women but not in men: the relative mtDNA copy number in elderly individuals varies significantly in both sexes, possibly due to age-related physiological changes. It is necessary to consider the gender and age of patients when using an indicator such as the level of extracellular DNA of blood serum for diagnosis and monitoring.

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Funding

The studies were carried out within the framework of the basic part of state assignment of the Ministry of Education and Science of the Russian Federation, R&D no. 1878 “The Development of Fundamental Aspects of Molecular Diagnostics and Mitochondrial Pharmacology.”

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    I. Yu. Mitroshina, N. P. Sirota & E. A. Kuznetsova

  2. Academy of Biology and Biotechnology named after D.I. Ivanovsky, Southern Federal University, 344090, Rostov-on-Don, Russia

    V. N. Prokofiev

Authors
  1. I. Yu. Mitroshina
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  2. N. P. Sirota
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  3. V. N. Prokofiev
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  4. E. A. Kuznetsova
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Corresponding author

Correspondence to E. A. Kuznetsova.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Translated by E. Makeeva

Abbreviations: %TDNA, DNA percentage in a comet tail; cfDNA, cell-free DNA; nDNA, nuclear DNA; mtDNA, mitochondrial DNA; qPCR, real-time (quantitative) polymerase chain reaction.

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Mitroshina, I.Y., Sirota, N.P., Prokofiev, V.N. et al. Levels of Circulating DNA in Blood Serum and DNA Damage in Leukocytes of Healthy Donors of Different Genders and Ages. BIOPHYSICS 66, 310–315 (2021). https://doi.org/10.1134/S0006350921020147

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