The Change of Electrical Properties of Blood Corpuscles under in vitro Mechanical Stress


Changes in the electrical properties of blood corpuscles that are experiencing mechanical stress modeled in vitro have been studied. An increase in the concentration of ATP molecules in the intercellular space in response to the mechanical effect of moving plasma layers both in the blood of healthy people and patients with acute lymphoblastic leukemia is demonstrated. The surface potential of red blood cells and platelets becomes more positive both in the blood of healthy people and in leukemia patients. In contrast, the negative charge of lymphocytes in healthy people decreases in response to mechanical stress but increases in the blood of patients with acute lymphoblastic leukemia.

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  1. 1

    D. Malec, Polish J. Pharmacol. Pharmacy 48 (5), 457 (1996).

    Google Scholar 

  2. 2

    C. Cekic and J. Linden, Nat. Rev. Immunol. 16 (3), 177 (2016).

    Article  Google Scholar 

  3. 3

    H. K. Eltzschig, M. V. Sitkovsky, and S. C. Robson, New Engl. J. Med. 367 (24), 2322 (2012).

    Article  Google Scholar 

  4. 4

    G. Burnstock and D. V. Francesco, Purinergic Signal. 9 (4), 491 (2013).

    Article  Google Scholar 

  5. 5

    M. Yang and W. J. Brackenbury, Front. Physiol. 4, 121 (2013).

    Article  Google Scholar 

  6. 6

    X. Zhang, Acta Biochim. Biophys. Sin. 41, 362 (2009).

    Article  Google Scholar 

  7. 7

    N. Montalbetti, M. F. L. Denis, O. P. Pignataro, and E. Kobatake, J. Biol. Chem. 286 (44), 38397 (2011).

    Article  Google Scholar 

  8. 8

    A. Baroja-Mazo, H. Barbero-Gremades, and P. Pelegrini, Biochem. Biophys. Acta 828, 79 (2013).

    Article  Google Scholar 

  9. 9

    F. Virgilio, Nature Rev. Cancer 18 (10), 601 (2018).

    Article  Google Scholar 

  10. 10

    T. Oonishi, K. Sakashita, and N. Uyesaka, Am. J. Physiol. Soc. 273, 1828 (1997).

    Article  Google Scholar 

  11. 11

    N. M. Titova, T. N. Zamai, and G. V. Borovkova, Biochemistry and Molecular Biology: A Laboratory Practical Course (Krasnoyarsk, 2008) [in Russian].

    Google Scholar 

  12. 12

    T. L. Aleinikova and G. V. Rubtsova, Guidelines for Practical Courses in Biological Chemistry (Vysshaya Shkola, Moscow, 1988) [in Russian].

    Google Scholar 

  13. 13

    K. I. Taborskaya, M. Yu. Frolova, and N. V. Kuleva, Tsitologiya 58 (2), 115 (2016).

    Google Scholar 

  14. 14

    E. A. Sladkova and M. Yu. Skorkina, Biophysics (Moscow) 59 (2), 254 (2014).

    Article  Google Scholar 

  15. 15

    J. Evans, W. Gratzer, N. Mohandas, et al., Biophys. J. 94 (10), 4134 (2008).

    Article  ADS  Google Scholar 

  16. 16

    D. E. Pafundo, C. I. Alvarez, C. A. Krumschnabel, and P. J. Schwarzbaum, J. Biol. Chem. 285, 6134 (2010).

    Article  Google Scholar 

  17. 17

    A. Kusumi, Annu. Rev. Biophys. Biomol. Struct. 34, 351 (2005).

    Article  Google Scholar 

  18. 18

    K. Kaczmarek-Hajek, E. Lorinczi, R. Hausmann, and A. Nicke, Purinergic Signal. 8 (3), 375 (2012).

    Article  Google Scholar 

  19. 19

    S. N. Orlov, Purinergic Signal. 3 (3), 231 (2007).

    Article  Google Scholar 

  20. 20

    X. Qian and L. Wen-jun, Cell Biochem. Biophys. 67 (3), 1473 (2013).

    Article  Google Scholar 

  21. 21

    T. Bose, A. Cieslar-Pobuda, and E. Wiechec, Cell Death Dis. 6 (2), 101 (2015).

    Google Scholar 

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This work was supported by the Russian Science Foundation, Studies Initiated by Young Scientists project, 2018–2020, progect no. 18-75-00041.

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Correspondence to E. A. Sladkova.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals: All manipulations with humans met the ethical standards of the Declaration of Helsinki, 1964, with later amendments. Informed consent was obtained from each participant of the study.

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Translated by V. Gulevich

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Sladkova, E.A. The Change of Electrical Properties of Blood Corpuscles under in vitro Mechanical Stress. BIOPHYSICS 65, 775–778 (2020).

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  • Keywords: mechanical stress
  • purinergic signaling system
  • surface potential
  • lymphocytes
  • red blood cells
  • platelets