Fluorimetric Analysis of the Impact of Coal Sludge Pollution on Phytoplankton


The results of biomonitoring of the Olkhovaya River (Donetsk oblast) are presented. The impact of mine sludge waste on the state of surface water was shown. The assessment of physical and chemical parameters made it possible to characterize the water in the Olkhovaya River as dirty and polluted with a large number of suspended coal particles, whose content significantly exceeded the maximum permissible standards. The decrease in the content of chlorophyll in water samples taken below the sludge drains into the riverbed was determined by the fluorimetric method. The results of analysis of chlorophyll fluorescence induction curves are presented. It was found that contamination by sludge drains leads to disruption of primary photosynthetic reactions in phytoplankton cells.

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The authors are grateful to E. I. Mironenko, senior teacher of the Department of Botany and Ecology of the Faculty of Biology of Donetsk National University, for assistance in determining the species composition of phytoplankton in the studied water samples.

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Correspondence to S. V. Chufitskiy.

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Conflict of interests. The authors declare that they have no conflicts of interest.Statement on the welfare of humans or animals. This article does not contain any studies involving animals performed by any of the authors.

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Translated by E. Puchkov

Abbreviations: SCIWP—specific combinatorial index of water pollution; MPC—maximum permissible concentration; PS— photosystem.

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Bespalova, S.V., Romanchuk, S.M., Chufitskiy, S.V. et al. Fluorimetric Analysis of the Impact of Coal Sludge Pollution on Phytoplankton. BIOPHYSICS 65, 850–857 (2020). https://doi.org/10.1134/S0006350920050024

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  • Keywords: fluorimetry
  • phytoplankton
  • sludge accumulators
  • bioindication