Abstract
Electron paramagnetic resonance spectroscopy has been applied for the first time to study the bio-distribution of magnetoliposomes formed with magnetite nanoparticles (Fe3O4) in tumors and organs of Lewis carcinoma-bearing mice in the absence and presence of an external magnetic field. The animals of the experimental group were subjected to an external magnetic field (0.6 T) in the tumor area after intravenous injection of magnetoliposomes at a dose of 7.56 Fe/kg. Analysis of the electron-spin resonance spectra of mouse organs and tissue samples showed that exposure to a magnetic field resulted in a two-fold increase in Fe3O4 accumulation within the tumor (p < 0.05) compared to the control; this makes it possible to recommend the obtained magnetoliposomes for use as a magnetically controlled carriers for targeted delivery of antitumor agents. A high concentration of superparamagnetic magnetite nanoparticles was detected in the liver in the absence and presence of an external magnetic field. The differences in the accumulation of Fe3O4 in the lungs and liver in the presence of a magnetic field were statistically insignificant.
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The work was performed on the topic of State Task No. 44.2 (Registration No. 001201253311).
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The authors declare that there is no conflict of interest when writing this article.
COMPLIANCE WITH ETHICAL STANDARDS
All procedures with animals were carried out in strict accordance with the European Convention for the protection of vertebrates used for experiments or other scientific purposes (1997).
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Translated by E. Puchkov
Abbreviations: SPIONs, superparamagnetic nanoparticles of magnetite; MLP, magnetoliposomes; EPR, electron paramagnetic resonance; MF, magnetic field.
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Marnautov, N.A., Serezhenkov, V.A., Komissarova, L.K. et al. Evaluation of the Biodistribution of Magnetoliposomes in a Tumor and Organs of Mice by Electron Paramagnetic Resonance Spectroscopy. BIOPHYSICS 65, 656–659 (2020). https://doi.org/10.1134/S0006350920040090
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DOI: https://doi.org/10.1134/S0006350920040090