Abstract
Ligand binding to DNA duplexes in a DNA biosensor was studied in conditions where the number of ligand molecules in the solution fluctuates under the influence of a fluctuating medium (external noise). A multiplicative stochastic differential equation was obtained for the case of low occupancy to describe the time-dependent change in the number of ligands absorbed on DNA duplexes. The average value and relaxation time of the output signal of a DNA biosensor were calculated as functions of ligand absorption on DNA duplexes in a fluctuating medium. Multiplicative noise was shown to decrease the average output signal of a DNA biosensor. Signal deterioration was found to have a threshold; i.e., the signal is not deteriorated at a certain ratio between the absorption parameters and the intensity of external noise. The relaxation time of the output signal of a DNA biosensor was shown to increase with the increasing intensity of external noise.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
S. K. Metkar and K. Girigoswami, Biocatal. Agric. Biotechnol. 17, 271 (2019).
M. S. Mufamadi and P. R. Sekhejane, in Nanotechnology. An Agricultural Paradigm (Springer, Singapore, 2017), pp. 263–278.
T. G. Drummond, M. G. Hill, and J. Barton, Nat. Biotechnol. 21 (10), 1192 (2003).
J. Wang, Anal. Chim. Acta 469, 63 (2002).
E. Palecek, M. Fojta, and F. Jelen, Bioelectrochemistry 56, 85 (2002).
Yu. K. Akhapkin and S. I. Bartsev, Bioengineering: A New Direction in Computerization (Nauka, Moscow, 1990) [in Russian].
V. Kavita, J. Bioengineer. Biomed. Sci. 7 (2), 222 (2017).
A. Halperin, A. Buhot, and E. B. Zhulina, J. Phys. Condens. Matter 18, S463 (2006).
B. A. Baker and V. T. Milam, Nucleic Acids Res. 39 (15), e99 (2011).
M. V. Golovkin, Yu. D. Nechipurenko, and G. V. Gursky, Biophysics (Moscow) 54 (4), 409 (2009).
Yu. D. Nechipurenko, in Physicochemical Mechanisms and Regulation of Energy Transformation in Biological Structures, Ed. by G. Yu. Riznichenko and A. B. Rubin (Inst. Comput. Res., Izhevsk, 2017), pp. 522–530 [in Russian].
A. T. Karapetyan, G. A. Avetisyan, and V. B. Arake-lya-n, Dokl. Nats. Akad. Nauk Armenii 110 (4), 376 (2010).
V. Arakelyan, Y. Babayan, and G. Potikyan, J. Biomol. Struct. Dyn. 18, 231 (2000).
W. Horsthemke and R. Lefever, Noise-Induced Transitions: Theory and Applications in Physics, Chemistry, and Biology (Springer, Bedlin, 1984; Mir, Moscow, 1987).
S. A. Akhmanov, Yu. E. Dyakov, and A. S. Chirkin, A Primer in Statistical Radiophysics and Optics (Nauka, Moscow, 1981) [in Russian].
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interests. The authors declare that they have no conflict of interest. This work does not contain any studies involving animals or human subjects performed by any of the authors.
Additional information
Translated by T. Tkacheva
Rights and permissions
About this article
Cite this article
Arakelyan, V.B., Antonyan, A.P., Parsadanyan, M.A. et al. Ligand Absorption on DNA Duplexes under the Influence of a Fluctuating Medium Changes the Output Signal of a DNA Biosensor. BIOPHYSICS 65, 569–573 (2020). https://doi.org/10.1134/S0006350920040028
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006350920040028