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The Effect of the Viscosity of a Trehalose Solution on ATP Hydrolysis by Chloroplast F1-ATPase

  • MOLECULAR BIOPHYSICS
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Abstract

The effect of trehalose solution viscosity on the kinetics of ATP hydrolysis by CF1-ATPase (pea chloroplast F1-ATPase) was studied. Trehalose added to the reaction mixture had a double effect: the Mg-dependent enzyme activity was stimulated at trehalose levels below 20 wt % and suppressed at higher trehalose levels. The Ca-dependent activity of CF1 decreases monotonically with the increasing trehalose level. It was shown that stimulation of the Mg-dependent enzyme activity was a result of diminished Mg-ADP-dependent enzyme inactivation. In the case of negligible inactivation, the elevated trehalose content caused a decrease in the maximum rate of ATP hydrolysis and the apparent Michaelis constant increased. The changes in the values of the Michaelis constant and enzyme activity indicate that the delivery of reaction substrates and the conformational changes in CF1-ATPase accompanying the hydrolysis are impeded by the viscosity of the medium. In accordance with Cramer’s rule, the energy loss due to interaction with the medium leads to the conclusion that the efficiency of energy conversion by CF1-ATPase never reaches 100%.

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Funding

This work was conducted in the framework of the state order no. AAAA-A17-117030110135-1.

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Moscow oblast, 142290, Pushchino, Russia

    N. S. Novichkova & A. N. Malyan

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  1. N. S. Novichkova
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  2. A. N. Malyan
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Correspondence to A. N. Malyan.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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The authors declare that they have no conflict of intere-st.

Additional information

Translated by E. V. Makeeva

Abbreviations: CF1, chloroplast coupling factor.

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Novichkova, N.S., Malyan, A.N. The Effect of the Viscosity of a Trehalose Solution on ATP Hydrolysis by Chloroplast F1-ATPase. BIOPHYSICS 64, 853–857 (2019). https://doi.org/10.1134/S0006350919060174

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  • DOI: https://doi.org/10.1134/S0006350919060174

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