, Volume 64, Issue 1, pp 89–94 | Cite as

The Biological Effect of Dinitrosyl Iron Complexes with Glutathione upon Nitric Oxide Hyperproduction Induced by Endotoxin Shock

  • A. A. TimoshinEmail author
  • V. L. Lakomkin
  • A. A. Abramov
  • A. F. Vanin
  • E. K. Ruuge

Abstract—The objective of this work was to study the biological effect of dinitrosyl iron complexes (DNICs) with the glutathione ligand (GSH−DNICs) as a stabilized form of nitric oxide in a rat model of nitric oxide hyperproduction induced by inflammation. Administration of GSH−DNICs in endotoxin shock did not increase the total nitric oxide levels in rat organs, but exerted a protective effect by suppressing nitric oxide hyperproduction in the liver and led to an accumulation of the complexes with protein ligands in the kidney.

Keywords: antioxidant inflammation dinitrosyl iron complexes nitric oxide spin trap electron paramagnetic resonance 



This work was supported by the Russian Foundation for Basic Research (project no. 18-015-00027).


Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


  1. 1.
    A. F. Vanin, Nitric Oxide: Biol. Chem. 21, 1 (2009).Google Scholar
  2. 2.
    A. F. Vanin, A. P. Poltorakov, V. D. Mikoyan, et al., Nitric Oxide: Biol. Chem. 23, 136 (2010).Google Scholar
  3. 3.
    Y. Suryo Rahmanto, D. S. Kalinowski, D. J. Lane, et al., J. Biol. Chem. 287 (10), 6960 (2012).CrossRefGoogle Scholar
  4. 4.
    A. F. Vanin, Nitric Oxide: Biol. Chem. 54, 156 (2016).Google Scholar
  5. 5.
    V. D. Mikoyan, E. N. Burgova, R. R. Borodulin, et al., Nitric Oxide: Biol. Chem. 62, 1 (2017).Google Scholar
  6. 6.
    A. F. Vanin, Biophysics (Moscow) 62 (4), 509 (2017).CrossRefGoogle Scholar
  7. 7.
    S.-C. Wu, C.-Y. Lu, Y.-L. Chen, et al., Inorg. Chem. 55 (18), 9383 (2016).CrossRefGoogle Scholar
  8. 8.
    C.-H. Ke, C.-H. Chen, M.-L. Tsai, et al., J. Am. Chem. Soc. 139 (1), 67 (2017).CrossRefGoogle Scholar
  9. 9.
    T.-T. Lu, Y.-M. Wang, C.-H. Hung, et al., Inorg. Chem. (2018).
  10. 10.
    K. B. Shumaev, A. L. Dudylina, M. V. Ivanova, et al., BioFactors 44 (3), 237 (2018).CrossRefGoogle Scholar
  11. 11.
    E. I. Chazov, O. V. Rodnenkov, A. V. Zorin, et al., Nitric Oxide: Biol. Chem. 26, 148 (2012).Google Scholar
  12. 12.
    V. L. Lakomkin, A. F. Vanin, A. A. Timoshin, et al., Nitric Oxide: Biol. Chem. 16, 413 (2007).Google Scholar
  13. 13.
    A. A. Timoshin, V. L. Lakomkin, A. A. Abramov, et al., Eur. J. Pharmacol. 765, 525 (2015).CrossRefGoogle Scholar
  14. 14.
    A. A. Timoshin, V. L. Lakomkin, A. A. Abramov, et al., Bull. Exp. Biol. Med. 162 (2), 207 (2016).CrossRefGoogle Scholar
  15. 15.
    K. B. Shumaev, A. A. Gubkin, V. A. Serezhenkov, et al., Nitric Oxide: Biol. Chem. 18, 37 (2008).Google Scholar
  16. 16.
    A. A. Timoshin, D. Yu. Drobotova, O. V. Tskitishvili, et al., Dokl. Biochem. Biophys. 432, 106 (2010).CrossRefGoogle Scholar
  17. 17.
    V. I. Kapelko, V. L. Lakomkin, A. A. Abramov, et al., Oxidative Medicine and Cellular Longevity 2017, Article ID 9456163 (2017).CrossRefGoogle Scholar
  18. 18.
    M. I. Remizova, N. I. Kochetygov, K. A. Gerbout, et al., Eur. J. Pharmacol. 662, 40 (2011).CrossRefGoogle Scholar
  19. 19.
    A. F. Vanin, L. A. Ostrovskaya, D. B. Korman, et al., Biophysics (Moscow) 60 (6), 963 (2015).CrossRefGoogle Scholar
  20. 20.
    K. Lepka, K. Voldracht, E. Bill, et al., Glia 65 (9), 1521 (2017).CrossRefGoogle Scholar
  21. 21.
    A. A. Timoshin, V. L. Lakomkin, A. A. Abramov, et al., Dokl. Biochem. Biophys. 462, 166 (2015).CrossRefGoogle Scholar
  22. 22.
    A. F. Vanin and A. A. Timoshin, Methods Mol. Biol. 704, 135 (2011).CrossRefGoogle Scholar
  23. 23.
    A. A. Timoshin, O. I. Pisarenko, V. L. Lakomkin, et al., Exp. Clin. Cardiol. 5 (2), 59 (2000).Google Scholar
  24. 24.
    R. Schreck, B. Meier, D. E. Mannel, et al., J. Exp. Med. 175, 1181 (1992).CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • A. A. Timoshin
    • 1
    Email author
  • V. L. Lakomkin
    • 1
  • A. A. Abramov
    • 1
  • A. F. Vanin
    • 2
  • E. K. Ruuge
    • 1
  1. 1.National Medical Research Center for Cardiology, Ministry of Health of the Russian FederationMoscowRussia
  2. 2.Semenov Institute of Chemical Physics, Russian Academy of SciencesMoscowRussia

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