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Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy

  • MOLECULAR BIOPHYSICS
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Abstract

A method to estimate the concentration of chemically accessible carboxylic groups on the surface of a polyethylene terephthalate (PET) film was developed using the fluorochrome cycanine Cy5 and digital fluorescence microscopy. A method was developed to activate the carboxylic groups on the PET surface and to allow covalent immobilization of amino group-containing molecular constructs.

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ACKNOWLEDGMENTS

We are grateful to R.A. Yurasov for help using the software program.

This work was supported by the Program for Basic Research at the State Academies of Sciences from 2013 to 2020 (project no. 01201363819, Development of New Methods for Molecular Diagnosis of Human and Animal Disorders).

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    R. A. Miftakhov, S. A. Lapa, V. E. Shershov, O. A. Zasedateleva, T. O. Guseinov, M. A. Spitsyn, V. E. Kuznetsova, D. D. Mamaev, Yu. P. Lysov, V. E. Barsky, E. N. Timofeev, A. S. Zasedatelev & A. V. Chudinov

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  1. R. A. Miftakhov
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  2. S. A. Lapa
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  3. V. E. Shershov
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  5. T. O. Guseinov
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  7. V. E. Kuznetsova
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  8. D. D. Mamaev
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  9. Yu. P. Lysov
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  12. A. S. Zasedatelev
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  13. A. V. Chudinov
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Corresponding author

Correspondence to A. V. Chudinov.

Additional information

Translated by T. Tkacheva

Abbreviations: PCR, polymerase chain reaction; PET, polyethylene terephthalate.

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Miftakhov, R.A., Lapa, S.A., Shershov, V.E. et al. Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy. BIOPHYSICS 63, 512–518 (2018). https://doi.org/10.1134/S0006350918040127

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  • DOI: https://doi.org/10.1134/S0006350918040127

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