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The Effect of Electromagnetic Radiation at Frequencies of 51.8 and 53.0 GHz on Growth, Pigment Content, Hydrogen Photoemission, and F0F1-ATPase Activity in the Purple Bacterium Rhodobacter sphaeroides

Biophysics volume 63, pages 351–356 (2018)Cite this article

Abstract

Exposure of the purple bacteria Rhodobacter sphaeroides MDC6522 isolated from Jermuk mineral springs (Armenia) to extremely high-frequency electromagnetic radiation (51.8 and 53.0 GHz) for 15 min resulted in a pronounced increase in the specific growth rate and H2 photoemission. However, a significant decrease in the specific growth rate (1.6–2.0 times) was observed when the duration of irradiation was prolonged to 1 h. The maximum effect was at a frequency of 53.0 GHz. During irradiation for 1 h, absorption maxima typical of carotenoids gradually disappeared, and the level of bacteriochlorophyll а complexes decreased. Prolonged irradiation also inhibited the H2 production during bacterial growth for 72 h, although it was restored after 96 h of growth. The activity of N,N'-dicyclohexylcarbodiimide-sensitive proton F0F1- ATPase also decreased in Rh. sphaeroides. These results indicate that the membrane-bound F0F1-ATPase may be the main target of action of extremely-high-frequency electromagnetic radiation. The data we obtained can be used in biotechnology for control of growth and hydrogen metabolism of phototrophic bacteria.

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Abbreviations

EHF EMR:

electromagnetic radiation of extremely high frequency

ORP:

oxidation-reduction potential

BCHL a :

bacteriochlorophyll a

DCCD:

N,N'-dicyclohexylcarbodiimide

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Authors and Affiliations

  1. Department of Biology, Yerevan State University, 0025, Yerevan, Armenia

    L. Gabrielyan, V. Kalantaryan & A. Trchounian

Authors
  1. L. Gabrielyan
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  2. V. Kalantaryan
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  3. A. Trchounian
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Correspondence to A. Trchounian.

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Original Russian Text © L. Gabrielyan, V. Kalantaryan, A. Trchounian, 2018, published in Biofizika, 2018, Vol. 63, No. 3, pp. 468–474.

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Gabrielyan, L., Kalantaryan, V. & Trchounian, A. The Effect of Electromagnetic Radiation at Frequencies of 51.8 and 53.0 GHz on Growth, Pigment Content, Hydrogen Photoemission, and F0F1-ATPase Activity in the Purple Bacterium Rhodobacter sphaeroides. BIOPHYSICS 63, 351–356 (2018). https://doi.org/10.1134/S0006350918030077

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  • DOI: https://doi.org/10.1134/S0006350918030077

Keywords

  • Rhodobacter sphaeroides
  • electromagnetic irradiation of extremely high frequency
  • growth of bacteria
  • oxidation-reduction potential
  • H2 production
  • F0F1-ATPase